Mechanism and biological relevance of blue-light (420–453 nm)-induced nonenzymatic nitric oxide generation from photolabile nitric oxide derivates in human skin in vitro and in vivo

Opländer, Christian; Deck, Annika; Volkmar, Christine M.; Kirsch, Michael; Liebmann, J.; Born, Matthias; Abeelen, Frank van; Faassen, Ernst E. van; Kröncke, Klaus‐Dietrich; Windolf, Joachim; Suschek, Christoph V.

doi:10.1016/j.freeradbiomed.2013.09.022

Cited by 83 publications

(71 citation statements)

References 48 publications

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“…Sodium nitrite potentiated vasodilation through direct photolysis of nitrite to NO [28]. In addition, blue light (420–450 nm) can release NO from the skin [29]. However, the practical use of light in these wavelengths is limited due to the minimal depth of penetration (0.1–0.2 mm) and the deleterious health effects of UV exposure to the skin [30, 31].…”

Section: Discussionmentioning

confidence: 99%

Red/near infrared light stimulates release of an endothelium dependent vasodilator and rescues vascular dysfunction in a diabetes model

Keszler

Lindemer

Weihrauch

et al. 2017

Free Radical Biology and Medicine

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Peripheral artery disease (PAD) is a morbid condition whereby ischemic peripheral muscle causes pain and tissue breakdown. Interestingly, PAD risk factors, e.g. diabetes mellitus, cause endothelial dysfunction secondary to decreased nitric oxide (NO) levels, which could explain treatment failures. Previously, we demonstrated 670nm light (R/NIR) increased NO from nitrosyl-heme stores, therefore we hypothesized R/NIR can stimulate vasodilation in healthy and diabetic blood vessels. Vasodilation was tested by ex vivo pressure myography in wild type C57Bl/6, endothelial nitric oxide synthase (eNOS) knockout, and db/db mice (10 mW/cm2 for 5min with 10 min dark period). NOS inhibition with N-Nitroarginine methyl ester (L-NAME) or the NO scavenger Carboxy-PTIO (c-PTIO) tested the specificity of NO production. 4,5-Diaminofluorescein diacetate (DAF-2) measured NO in human dermal microvascular endothelial cells (HMVEC-d). R/NIR significantly increased vasodilation in wild type and NOS inhibited groups, however R/NIR dilation was totally abolished with c-PTIO and blood vessel denudation. Interestingly, the bath solution from intact R/NIR stimulated vessels could dilate light naïve vessels in a NO dependent manner. Characterization of the bath identified a NO generating substance suggestive of S-nitrosothiols or non heme iron nitrosyl complexes. Consistent with the finding of an endothelial source of NO, intracellular NO increased with R/NIR in HMVEC-d treated with and without L-NAME (1mM), yet c-PTIO (100μm) reduced NO production. R/NIR significantly dilated db/db blood vessels. In conclusion, R/NIR stimulates vasodilation by release of NO bound substances from the endothelium. In a diabetes model of endothelial dysfunction, R/NIR restores vasodilation, which lends the potential for new treatments for diabetic vascular disease.

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Section: Discussionmentioning

confidence: 99%

Red/near infrared light stimulates release of an endothelium dependent vasodilator and rescues vascular dysfunction in a diabetes model

Keszler

Lindemer

Weihrauch

et al. 2017

Free Radical Biology and Medicine

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“…Short visible wavelengths are often reported to photolytically generate NO and ROS from nitrosated proteins and NADPH oxidase, respectively. These can then modulate cell metabolic activity, vasodilatation and improve wound healing . IR light has been shown to activate epidermal keratinocytes in vitro and to induce ROS production in mouse skin .…”

Section: Multiple Downstream Biomolecular Reactions Explaining Physiomentioning

confidence: 99%

Photobiomodulation devices for hair regrowth and wound healing: a therapy full of promise but a literature full of confusion

Mignon

Botchkareva

Uzunbajakava

et al. 2016

Experimental Dermatology

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Photobiomodulation is reported to positively influence hair regrowth, wound healing, skin rejuvenation and psoriasis. Despite rapid translation of this science to commercial therapeutic solutions, significant gaps in our understanding of the underlying processes remain. The aim of this review was to seek greater clarity and rationality specifically for the selection of optical parameters for studies on hair regrowth and wound healing. Our investigation of 90 reports published between 1985 and 2015 revealed major inconsistencies in optical parameters selected for clinical applications. Moreover, poorly understood photoreceptors expressed in skin such as cytochrome c oxidase, cryptochromes, opsins etc. may trigger different molecular mechanisms. All this could explain the plethora of reported physiological effects of light. To derive parameters for optimal clinical efficacy of photobiomodulation, we recommend a more rational approach to underpin clinical studies, with research on molecular targets and pathways using well-defined biological model systems to enable translation of optical parameters from in vitro to in vivo. Furthermore, special attention needs to be paid when conducting studies for hair regrowth, aiming for double-blind, placebocontrolled randomized clinical trials as the gold standard for quantifying hair growth.

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“…Photobiomodulation (PBM) as a clinical application for wound healing is impaired by a lack of complete understanding of all involved molecular targets , which include cytochrome c oxidase (CCO) , nitrozated proteins , flavoproteins generating reactive oxygen species (ROS) and light‐activated calcium channels . Previously it was reported that interaction of CCO with red light induces a shift in the redox potential of the cell, inducing transcriptional changes modulating proliferation, metabolism and motility, all important in wound healing .…”

Section: Introductionmentioning

confidence: 99%

Does blue light restore human epidermal barrier function via activation of Opsin during cutaneous wound healing?

et al. 2018

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Opsins are expressed in the epidermis and dermis of human skin and in the newly regenerating epidermis following wounding. An increase in OPN3 expression in the epithelial tongue may be a potential mechanism for the stimulation of wound closure by blue light. Since keratinocytes and fibroblasts retain their expression of Opsins in culture, they provide a good model to investigate the mechanism of blue light in wound healing responses. Knockdown of OPN3 led to a reduction in early differentiation of keratinocytes following irradiation with blue light, suggesting OPN3 is required for restoration of the barrier function. Understanding the function and relationship of different photoreceptors and their response to specific light parameters will lead to the development of reliable light-based therapies for cutaneous wound healing. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.

show abstract

Mechanism and biological relevance of blue-light (420–453 nm)-induced nonenzymatic nitric oxide generation from photolabile nitric oxide derivates in human skin in vitro and in vivo

Cited by 83 publications

References 48 publications

Red/near infrared light stimulates release of an endothelium dependent vasodilator and rescues vascular dysfunction in a diabetes model

Red/near infrared light stimulates release of an endothelium dependent vasodilator and rescues vascular dysfunction in a diabetes model

Photobiomodulation devices for hair regrowth and wound healing: a therapy full of promise but a literature full of confusion

Does blue light restore human epidermal barrier function via activation of Opsin during cutaneous wound healing?

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