Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

Xu, Zengping; Gao, Yuxiang; Meng, Sheng; Yang, Baochen; Pang, Liyun; Wang, Chen; Liu, Tianjun

doi:10.3389/fmicb.2016.00242

Cited by 47 publications

(55 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The images stained by PI verified the antibacterial activity by carbazole derivatives BMEPC and BMEMC. Compared with the previously reported photosensitizers, 33 , 34 the MIC value of BMEPC is relatively lower under irradiation, indicating a more prominent effect on bacterial inhibition. These results suggest that photoinduced active species are the dominant factors to kill bacteria.…”

Section: Resultscontrasting

confidence: 72%

Laser-Induced Antibacterial Activity of Novel Symmetric Carbazole-Based Ethynylpyridine Photosensitizers

Chen

Zheng

et al. 2018

ACS Omega

View full text Add to dashboard Cite

In this study, two kinds of novel carbazole-based ethynylpyridine salts: 3,6-bis[2-(1-methylpyridinium)ethynyl]-9-pentyl-carbazole diiodide (BMEPC) and 3,6-bis[2-(1-methylpyridinium)ethynyl]-9-methyl-carbazole diiodide (BMEMC) have been employed as photosensitizers owing to their excellent antibacterial activity. These molecules possess symmetric A−π–D−π–A-type structures, which would bring in the unique optical properties. The inhibition zone measurement of a gradient concentration from 0 to 100 μM showed BMEPC and BMEMC photoinduced antibacterial activity against Escherichia coli. Diameters of zone of inhibition were up to 15 and 14 mm under laser irradiations. Under the exposure of the laser of 442 nm with a power density of 20 mW/cm2, the minimum inhibitory concentrations (MICs) of BMEPC on E. coli were between 3.5 and 6.9 μM and that of BMEMC were between 9.4 and 18.8 μM, respectively. In the dark experiments as a control, the MIC value is between 6.9 and 13.8 μM for BMEPC, whereas it is between 187.5 and 225.0 μM for BMEMC. By the comparison of the MIC values of BMEPC and BMEMC with laser irradiation and in dark, the laser-induced toxicity on bacteria is more evident, though both of the derivatives have dark toxicity. With the laser irradiation duration of 30 s and 10 min for BMEPC and BMEMC, respectively, the survival rate of E. coli approximates zero. An antibacterial mechanism has been proposed based on the electron paramagnetic resonance characterization, which indicates that a nitride radical is generated under laser irradiation. The carbazole-based ethynylpyridine photosensitizers would provide high potential for further applications in photodynamic therapy.

show abstract

Section: Resultscontrasting

confidence: 72%

Laser-Induced Antibacterial Activity of Novel Symmetric Carbazole-Based Ethynylpyridine Photosensitizers

Chen

Zheng

et al. 2018

ACS Omega

View full text Add to dashboard Cite

show abstract

“…The wound healing rate decreased in the order of 100 μM > 200 μM > 50 μM, which parallels the order of the bactericidal effect. This suggests that PPIX-ED-PACT accelerates wound healing via bactericidal effects against P. aeruginosa [22,27,33]. The CFUs of P. aeruginosa in the wound and the wound healing rate in vivo revealed that PPIX-ED-PACT is efficient in the treatment of bacterial infection, and the therapeutic effect of PPIX-ED was better at 100 μM than at 50 or 200 μM.…”

Section: Plos Onementioning

confidence: 91%

“…The photodynamic effects of different photosensitizers on P. aeruginosa have been demonstrated [20][21][22][23]. In vivo, PACT has been primarily used to improve wound healing.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds

Zhao

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

This study examined the antibacterial effect of protoporphyrin IX-ethylenediamine derivative (PPIX-ED)-mediated photodynamic antimicrobial chemotherapy (PPIX-ED-PACT) against Pseudomonas aeruginosa in vitro and in vivo. PPIX-ED potently inhibited the growth of Pseudomonas aeruginosa by inducing reactive oxygen species production via photoactivation. Atomic force microscopy revealed that PPIX-ED-PACT induced the leakage of bacterial content by degrading the bacterial membrane and wall. As revealed using acridine orange/ethidium bromide staining, PPIX-ED-PACT altered the permeability of the bacterial membrane. In addition, the antibacterial effect of PPIX-ED-PACT was demonstrated in an in vivo model of P. aeruginosa-infected wounds. PPIX-ED (100 μM) decreased the number of P. aeruginosa colony-forming units by 4.2 log 10. Moreover, histological analysis illustrated that the wound healing rate was 98% on day 14 after treatment, which was 10% higher than that in the control group. According to the present findings, PPIX-ED-PACT can effectively inhibit the growth of P. aeruginosa in vitro and in vivo.

show abstract

“…The mechanism is based on the photochemical reactions of photosensitizers (PSs). Upon irradiation with light whose wavelength matches the absorption of the PSs, hydroxyl radicals, superoxides, or singlet oxygen ( 1 O 2 ) can be generated, causing damage to cell membranes and DNA molecules …”

Section: Nanomaterials For Antibiotic‐free Antibacterial Applicationsmentioning

confidence: 99%

Antibiotic‐Free Antibacterial Strategies Enabled by Nanomaterials: Progress and Perspectives

et al. 2019

View full text Add to dashboard Cite

The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adma.201904106.Bacterial infection is one of the top ten leading causes of death globally and the worst killer in low-income countries. The overuse of antibiotics leads to ever-increasing antibiotic resistance, posing a severe threat to human health. Recent advances in nanotechnology provide new opportunities to address the challenges in bacterial infection by killing germs without using antibiotics. Antibiotic-free antibacterial strategies enabled by advanced nanomaterials are presented. Nanomaterials are classified on the basis of their mode of action: nanomaterials with intrinsic or light-mediated bactericidal properties and others that serve as vehicles for the delivery of natural antibacterial compounds. Specific attention is given to antibacterial mechanisms and the structureperformance relationship. Practical antibacterial applications employing these antibiotic-free strategies are also introduced. Current challenges in this field and future perspectives are presented to stimulate new technologies and their translation to fight against bacterial infection.

show abstract

Mechanism and In Vivo Evaluation: Photodynamic Antibacterial Chemotherapy of Lysine-Porphyrin Conjugate

Cited by 47 publications

References 42 publications

Laser-Induced Antibacterial Activity of Novel Symmetric Carbazole-Based Ethynylpyridine Photosensitizers

Laser-Induced Antibacterial Activity of Novel Symmetric Carbazole-Based Ethynylpyridine Photosensitizers

Photodynamic antimicrobial chemotherapy in mice with Pseudomonas aeruginosa-infected wounds

Antibiotic‐Free Antibacterial Strategies Enabled by Nanomaterials: Progress and Perspectives

Contact Info

Product

Resources

About