Mechanism for the Endocytosis of Spherical Nucleic Acid Nanoparticle Conjugates*

Choi, Chung Hang Jonathan; Hao, Liang; Narayan, Suguna P.; Auyeung, Evelyn; Mirkin, Chad A.

doi:10.1201/9781003056676-21

Cited by 39 publications

(59 citation statements)

References 10 publications

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“…TWIST1 SmartFlare detects TWIST1 mRNA after 6 h using a concentration of 50 pM in human BMSCs SmartFlare probes enter the cell via endocytosis and this process can vary between different cell types (Choi et al 2013). The probe incubation time and concentration which is suggested by the manufacturer is 16 h and 100 pM.…”

Section: Resultsmentioning

confidence: 99%

Sorting living mesenchymal stem cells using a TWIST1 RNA-based probe depends on incubation time and uptake capacity

et al. 2019

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Bone marrow derived mesenchymal stromal cells (BMSCs) are multipotent progenitors of particular interest for cell-based tissue engineering therapies. However, one disadvantage that limit their clinical use is their heterogeneity. In the last decades a great effort was made to select BMSC subpopulations based on cell surface markers, however there is still no general consensus on which markers to use to obtain the best BMSCs for tissue regeneration. Looking for alternatives we decided to focus on a probe-based method to detect intracellular mRNA in living cells, the SmartFlare technology. This technology does not require fixation of the cells and allows us to sort living cells based on gene expression into functionally different populations. However, since the technology is available it is debated whether the probes specifically recognize their target mRNAs. We validated the TWIST1 probe and demonstrated that it specifically recognizes TWIST1 in BMSCs. However, differences in probe concentration, incubation time and cellular uptake can strongly influence signal specificity. In addition we found that TWIST1 high expressing cells have an increased expansion rate compared to TWIST1 low expressing cells derivedfrom the same initial population of BMSCs. The SmartFlare probes recognize their target gene, however for each probe and cell type validation of the protocol is necessary. 123Cytotechnology (2020) 72:37-45 https://doi.org/10.1007/s10616-019-00355-w( 0123456789().,-volV) (0123456789().,-volV)

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Section: Resultsmentioning

confidence: 99%

Sorting living mesenchymal stem cells using a TWIST1 RNA-based probe depends on incubation time and uptake capacity

et al. 2019

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“…Subsequently, 3% HCl (0.3 ml) in concentrated HNO3 was used to digest the cells at room temperature overnight, followed by the addition of indium at 5 ll of 5-ppm (as the internal standard), and 5 ml of matrix solution (2% HCl and 2% HNO3). CuO NPs contents in individual samples were determined using spectrophotometry at 280 nm with an atomic absorption device (Model 603, Perkins Elmer, Waltham, MA), and comparisons were made with a standard curve [24].…”

Section: Measurement Of Cuo Np Uptakementioning

confidence: 99%

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

Ghaleh

Zarei

Motlagh

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The aim of this paper was examining the combined impacts of CuO nanoparticles (CuO NPs), hyperthermia (H), and irradiation (R) on an increment of MCF-7 cells. The MTT assay was employed to assess the antiproliferative effects of CuO NPs (25, 50, and 100 lg/ml), hyperthermia (41 C for 1 h), and irradiation (200 cGy). Moreover, the perniciousness was estimated through the survival capability of cells, and apoptosis, ROS production, and levels of caspase-3,-8 and-9 proteins were determined. A significant (p < .01) decrease in proliferation index (0.124 ± 0.021), a significant (p < .01) increase in apoptosis (42% ± 1.54) of MCF7 cells, a significant (p < .03) increase in ROS formation (32.16 ± 1.9) and a significant (p < .01) increase in LDH release (33.28 ± 1.56) were recorded in the adjacency of MCF-7 cells by a combination of CuO NPs (100 mg/ml) and R þ H compared to control and other treatments. The activities of caspase-3 (0.33 ± 0.014) and caspase-9 (0.389 ± 0.019) also increased significantly (p < .05). However, caspase-8 showed no significant changes in its activity (p ¼ .065). Based on these observations, a combination of CuO NPs, hyperthermia, and irradiation could suppress the growth of MCF-7 cells and evoke cell apoptosis via mitochondrial membrane potential.

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“…Although Chad A. Mirkin et al reported that high density oligonucleotides on the surface of nanoparticles could bind scavenger receptors on the surface of macrophages and promote nanoparticles uptake into macrophages [10], in an attempt to further explore the endocytic pathways and investigate the mechanism of nanoparticles uptake in DCs which is important for initiating immune responses for vaccines, we pretreated BMDCs with methyl-β-cyclodextrin (MBCD), chlorpromazine (CPM) and fucoidan (FCD), respectively. MBCD can remove cholesterol from the cell membrane effectively inhibiting caveolindependent endocytosis, CPM can block the formation of clathrin-coated pits, while FCD is a pharmacological inhibitor of class A scavenger receptor (SR-A) [15][16][17][18].…”

Section: Endocytosis Mechanism Of Navsmentioning

confidence: 99%

“…Generally, nanovaccine carriers are required to encapsulate and codelivery antigens and adjuvants to overcome intrinsic di culties of functional cargos and trigger delightful immune responses [4][5][6]. Recently, it has been reported that nucleic acids arranging in a dense spherical spatial form (SNAs) on a core made of gold or other polymers, can endow them unique properties, such as rapidly uptake through receptor-mediated mechanism and e cient protection against nuclease degradation [7][8][9][10]. However, the preparation of popular polymeric nanocarriers is complicated and the traditional core polymers have potential physiological toxicity [6,[11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

A simple self-adjuvanting biomimetic nanovaccine self-assembled with the conjugate of phospholipids and nucleotides can induce a strong cancer immunotherapeutic effect

Liú

Liu

et al. 2021

Biomater. Sci.

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Mechanism for the Endocytosis of Spherical Nucleic Acid Nanoparticle Conjugates*

Cited by 39 publications

References 10 publications

Sorting living mesenchymal stem cells using a TWIST1 RNA-based probe depends on incubation time and uptake capacity

Sorting living mesenchymal stem cells using a TWIST1 RNA-based probe depends on incubation time and uptake capacity

Using CuO nanoparticles and hyperthermia in radiotherapy of MCF-7 cell line: synergistic effect in cancer therapy

A simple self-adjuvanting biomimetic nanovaccine self-assembled with the conjugate of phospholipids and nucleotides can induce a strong cancer immunotherapeutic effect

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