Mechanism involved in the Circulation of oxidized LDL and its Ratio; An Early Risk Marker in Diabetic and Non-Diabetic Subjects with Coronary Heart Disease

Abstract

Under oxidative stress hardening of arteries is linked to oxidative variations in low density lipoproteins and imaginably more than one mechanism is involved in the atherosclerosis, where LDL is oxidized in all the cells of intimal wall during the progression of CHD. Ox-LDL act as a prognosticator of dysfunction in endothelium along with pro-thrombotic, pro-apoptotic, pro-inflammatory properties in subjects suffering from oxidative stress. Circulating ox-LDL is associated with the development of atherosclerosis but also numerous degenerative and age related disease. The objective of our study is to assess the levels of circulating oxidized LDL and its ratio in Diabetic and Non-Diabetic Subjects with CHD. This cross-sectional study was conducted in Department of General Medicine and Master Health check-up OP of SRM Medical College and Research Centre, Tamil Nadu, India. Totally 273 subjects in that 91 CHD patient without Diabetes, 91 CHD patient with diabetes and 91 healthy control in age group of 30 to 55 years and were age and sex matched. After overnight fasting blood samples were collected for analysis. ox-LDL were measured by ELISA method and Lipid Profile is measured using Auto Analyser AU480. Statistical analysis was done using student ‘t’ test and Pearson correlation analysis for the comparison between two groups. When compared to controls the mean level Low Density Lipoprotein and Plasma Oxidized LDL was significantly elevated in CHD group. Significantly positive correlation was observed between plasma oxidized LDL an LDL. The study concludes that increased circulating ox-LDL and its ratio are early risk marker and useful predictor of mortality in patients with CHD.For the diagnosis and treatment of coronary heart disease an appropriate method that reveal the mechanisms which increase circulating LDL and ox-LDL is needed.