The mechanism by which circulating ox-LDL and inflammatory indicators such as high sensitivity C-reactive protein may exert their impact on the development of CHD is still poorly understood. The study aims to measure the levels of circulating oxidized LDL and high sensitive C - reactive protein in CHD subjects. This cross-sectional study was conducted in the Department of Cardiology, General Medicine, and Master health check-up OP of SRM Medical college hospital and research centre, Tamil Nadu, India. A total of 182 subjects in which 91 CHD subjects and 91 healthy control in the age group of 30 to 55. ox-LDL and hs-CRP were measured by ELISA method and Lipid Profile is measured using Auto Analyzer AU480. Statistical analysis was done using the student ‘t’ test and Pearson correlation analysis was done for the comparison between two groups. The mean level ox-LDL and hs-CRP in CHD were elevated and statistically significant (p-value <0.001) compared to healthy controls. Ox-LDL was positively correlated with and hs-CRP. This study brings new insights that Ox-LDL and CRP may play a direct role in promoting the inflammatory component of atherosclerosis. Furthermore, more emphasis should be placed on these oxidative stress indicators in the prevention and treatment of CHD.

Under oxidative stress hardening of arteries is linked to oxidative variations in low density lipoproteins and imaginably more than one mechanism is involved in the atherosclerosis, where LDL is oxidized in all the cells of intimal wall during the progression of CHD. Ox-LDL act as a prognosticator of dysfunction in endothelium along with pro-thrombotic, pro-apoptotic, pro-inflammatory properties in subjects suffering from oxidative stress. Circulating ox-LDL is associated with the development of atherosclerosis but also numerous degenerative and age related disease. The objective of our study is to assess the levels of circulating oxidized LDL and its ratio in Diabetic and Non-Diabetic Subjects with CHD. This cross-sectional study was conducted in Department of General Medicine and Master Health check-up OP of SRM Medical College and Research Centre, Tamil Nadu, India. Totally 273 subjects in that 91 CHD patient without Diabetes, 91 CHD patient with diabetes and 91 healthy control in age group of 30 to 55 years and were age and sex matched. After overnight fasting blood samples were collected for analysis. ox-LDL were measured by ELISA method and Lipid Profile is measured using Auto Analyser AU480. Statistical analysis was done using student ‘t’ test and Pearson correlation analysis for the comparison between two groups. When compared to controls the mean level Low Density Lipoprotein and Plasma Oxidized LDL was significantly elevated in CHD group. Significantly positive correlation was observed between plasma oxidized LDL an LDL. The study concludes that increased circulating ox-LDL and its ratio are early risk marker and useful predictor of mortality in patients with CHD.For the diagnosis and treatment of coronary heart disease an appropriate method that reveal the mechanisms which increase circulating LDL and ox-LDL is needed.

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NO is a free radical gas, identified as Endothelial Derived Releasing Factor (EDRF) and synthesized from L-arginine which plays a dynamic role in defence against onset and development of coronary heart disease. Decreased availability of nitric oxide is one of the important cause for the pathogenesis of atherosclerosis. An accumulation of Low Density Lipoprotein cholesterol (LDL) in the intimal wall is the early step in atherosclerosis. Reduced NO level in the endothelium makes it vulnerable and increase the passage of leukocyte and undergo LDL oxidation in the sub endothelial space which ultimately lead to coronary heart disease. The aim of the study is to assess role of Nitric Oxide and oxidized LDL in CHD subjects. This cross-sectional study was conducted in SRM Medical college Hospital and Research centre on subjects attending the Department of Cardiology and Medicine OP. The study was conducted on 194 subjects in age group <40 years and were age and sex matched. After overnight fasting blood samples were collected for analysis for Lipid Profile and Nitric oxide and ox-LDL. Nitric Oxide and ox-LDL measured by ELISA method. Statistical analysis was done using Student‘t’ test and Pearson correlation analysis for the comparison between two groups. The mean level of Nitric Oxide were decreased (12.97± 1.20) significantly in CHD group compared to controls (19.08±4.74) (p <0.001). And Oxidized LDL showed significant increase (41.53± 8.72) in CHD group when compared to controls (16.73± 3.55) (p <0.001). Reduction in the levels of NO in the arterial wall impairs endothelial function. The study concludes that there is a strong association between NO and ox-LDL in progression of CHD. Interestingly, these analyses can help as diagnostic and monitoring markers in young CHD patients.

Nitric Oxide (NO) where produced by endothelial nitric oxide synthase (eNOS) enzyme which are inhibited by C-reactive protein (CRP) which causes endothelial dysfunction and cardiovascular events. In the current study, we evaluated the association of NO with hs-CRP in subjects with coronary heart disease. This Case-Control study was conducted 60 CHD patients and 60 healthy controls in age group of 30 to 55 years at SRM Medical College Hospital and Research Centre on subjects attending the Cardiology and medicine OP. Blood samples were collected after overnight fasting for analysis of Lipid Profile, High sensitive C-reactive protein. Nitric Oxide and High sensitive C-reactive protein is measured by ELISA method and Lipid Profile is measured using Auto Analyzer AU480. Statistical analysis was done using Student ‘t’ test and Pearson correlation analysis used to the variable between two groups. The mean level of LDL-C (161.9±27.46) and hs-CRP (6.80±1.35) were significantly elevated in CHD subjects when compared to the normal healthy controls. And the mean level of Nitric Oxide (12.97±1.20) were decreased significantly in CHD group when compared to controls. Increased oxidative stress associated with low grade inflammation lead to diminished bioavailability of nitric oxide.