The mechanism by which circulating ox-LDL and inflammatory indicators such as high sensitivity C-reactive protein may exert their impact on the development of CHD is still poorly understood. The study aims to measure the levels of circulating oxidized LDL and high sensitive C - reactive protein in CHD subjects. This cross-sectional study was conducted in the Department of Cardiology, General Medicine, and Master health check-up OP of SRM Medical college hospital and research centre, Tamil Nadu, India. A total of 182 subjects in which 91 CHD subjects and 91 healthy control in the age group of 30 to 55. ox-LDL and hs-CRP were measured by ELISA method and Lipid Profile is measured using Auto Analyzer AU480. Statistical analysis was done using the student ‘t’ test and Pearson correlation analysis was done for the comparison between two groups. The mean level ox-LDL and hs-CRP in CHD were elevated and statistically significant (p-value <0.001) compared to healthy controls. Ox-LDL was positively correlated with and hs-CRP. This study brings new insights that Ox-LDL and CRP may play a direct role in promoting the inflammatory component of atherosclerosis. Furthermore, more emphasis should be placed on these oxidative stress indicators in the prevention and treatment of CHD.

Introduction Diabetes mellitus and dyslipidemia are non-communicable diseases that can be prevented and controlled by maintaining a healthy lifestyle. Knowledge, attitude, and practice (KAP) in patients will provide information of much importance in Advocacy (to set guidelines by the Healthcare providers), Communication (increase awareness in subpopulation), and Social mobilization (improve services and expand community support). The study aimed to assess the effectiveness of an educative session on Knowledge, attitude, and practice (KAP) in patients attending a private medical college hospital on diabetes and dyslipidemia in the South-Indian population. Methodology The study was cross-sectional which included 100 patients (41 female and 59 male) of 18-50 years of age attending the hospital and the Central clinical laboratory for various disease conditions. A self-administered questionnaire on personal aspects and diabetes and dyslipidemia was collected. Result Among the study participants, 18% completed school education, 74% completed under-graduation, and 8% completed post-graduation. The frequency of livelihood showed 84% from rural and 16% from an urban background. Nearly 71% were active and 29% were sedentary performing self-activity. The source of information regarding the treatment of patients was also obtained from the patients to implement the same in advocacy. The knowledge of the study group on various aspects including the causes, symptoms, tests, complications of diabetes mellitus, lipid profile, dietary fiber, and exercises was analyzed. We found clarity of explanation about diabetes improved well after the educative session. Around 80% of responders believed that both fiber and routine exercises would contribute to preventing diabetes. Analysis of the attitude and practice revealed a significant knowledge of practicing proper diet, exercises, and routine health care after the educative session. Conclusion We conclude that healthcare providers shall focus on educating the patients according to their needs with the knowledge to have positive attitudes on healthy lifestyle practices in addition to the appropriate treatment.

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The use of HbA1c in diagnosis of diabetes mellitus by WHO and ADA has come under scrutiny. Aim: To study the erythrocyte index alterations and their relationship with HbA1c for proper interpretation of HbA1c values in diabetic subjects. Materials and methods: Data from 301 diabetic patients was collected. The parameters included were fasting plasma glucose, red blood cell count, hemoglobin level, hematocrit, glycated Hemoglobin (HbA1c), MCV, MCH, MCHC and RDW. Results: A negative correlation was found between HbA1c and MCV, MCH and MCHC and positive correlation with RDW. Conclusion: Hematological parameters like MCH, MCV and MCHC should be taken into account in interpreting HbA1c levels in diagnosis and management of pre diabetes and diabetes.

Glucose is the most common parameter to be estimated in a clinical laboratory. However, being highly unstable, the reported values often lead to under diagnosis of diabetes mellitus. This exerts a greater pressure on the laboratories as this reflects their quality. The aim of the study was to analyse the stability of the parameter in serum and plasma samples in a time delay of 1 hour and 2 hours in a tertiary care laboratory in South-India. The study included 125 samples of serum and plasma. Glucose was estimated by Hexokinase method. The serum and plasma samples were separated following the standard operating procedures of the laboratory. They were grouped as A and B. The samples of Group A and B were estimated for glucose concentration at 0, 1 and 2 hours and the values were noted against the time. The serum samples were stored at 2-4 degrees Celsius in between the estimations at 1 and 2 hours. The glucose concentration at 0, 1 and 2 hours were compared to each other with the help of Analysis of variance (ANOVA). The significance p of the ANOVA test performed to compare serum glucose concentrations analyzed at 0, 1 and 2 hours was found to be 1.000, with a p-value of 0.999 for the plasma sample glucose concentrations. It was evident that glucose concentration was stable with the p value closer to 1.000 in plasma samples estimated at 0, 1 and 2 hours. Plasma can be replaced with serum during the first hour without much alterations in the glucose concentration when stored at 2-4 degrees Celsius.