The cobalt, copper, and iron contents of a vertical series of water samples from Linsley Pond are given. Cobalt and iron are higher in the hypolimnion than in the epilimnion, but copper is not, The cobalt and iron content of the seston is estimated to be about equal to the content in normal soils, but the copper content is much higher. Vitamin B12 only exists in the water at concentrations lower than the optimum for some algae in culture. The proportion of the total cobalt tied up in vitamin B12 in the epilimnion is about 10% and about 4% or less in the hypolimnion.