Mechanism of Antiactivation at the Pseudomonas sp. Strain ADP σ^N-Dependent PatzTPromoter

Abstract

PatzT is an internal promoter of the atzRSTUVW operon that directs the synthesis of AtzT, AtzU, AtzV, and AtzW, components of an ABC-type cyanuric acid transport system. PatzT is IMPORTANCEHere, we describe a unique mechanism by which the regulatory protein AtzR prevents the activation of the N -dependent promoter PatzT. Promoters of this family are always positively regulated, but there are a few examples of overlapping negative regulation. The mechanism described here is highly unconventional and involves an interaction between the repressor and activator proteins to prevent the action of the repressor protein on the RNA polymerase-promoter complex.

Identification of a complete dibenzothiophene biodesulfurization operon and its regulator by functional metagenomics

“…Then, increasing concentrations of DszR were added to the mixture, and incubated at 30ºC for 10 min. E. coli IHF was a gift from Fernando Govantes and Ana Platero (Platero et al, 2016). P. putida N was a gift from Victoria Shingler.…”