Mechanism of bacteriophage conversion of lipase activity in Staphylococcus aureus

Lee, Chia-Yen; Iandolo, John J.

doi:10.1128/jb.164.1.288-293.1985

Cited by 51 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The temperate phages whose recombination functions have been studied to date can be divided into two groups based on the organization of these functions. In the majority of these phages, including k. its close relatives and the S. aureus serotype B phages ilii 1 (Novick, 1967) and L54a (Duval-iflah, 1972;Lee and landolo, 1985), the site-specific recombination functions are clustered tightly in the phage DNA. in the order attP, int anti xis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Novel organization of the site‐specific integration and excision recombination functions of the Staphylococcus aureus serotype F virulence‐converting phages φ13 and φ42

Carroll

Kehoe

Cavanagh

et al. 1995

Molecular Microbiology

View full text Add to dashboard Cite

Functions required for site-specific integration and excision of the Staphylococcus aureus serotype F virulence-converting phages phi 13 and phi 42 were localized and characterized. Like other temperate phages, integration of phi 13 and phi 42 sequences was found to require the product of an int gene located close to the phage attP site. Both int genes are almost identical, express proteins possessing characteristic features of the Int (integrase) family of recombinases, but share very little homology with previously described int genes, including those of the serotype B S. aureus phages L54a and phi 11. Nevertheless, all four S. aureus phages share an almost identical short sequence located immediately 5' to these distinct int genes, suggesting a common mechanism of int gene regulation. Upstream from these common sequences, the sequences of phi 13 and phi 42 are quite distinct from each other, and from the corresponding regions of phi 11 and L54a which encode the Xis proteins that are required with Int to mediate site-specific excision of the latter phages. Surprisingly, phi 13 and phi 42 sequences encompassing the attP sites and int genes, but lacking either an adjacent or more distant phage excision protein gene, were sufficient to mediate site-specific excision of integrated phage DNA sequences.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Bacteriophage catTiage is common in S. aureus, in which lysogenic conversion controls the expression of a variety of established and potential virulence factors (Betley and Mekalanos, 1985;Lee and landolo. 1985;Coieman etai.…”

Section: Introductionmentioning

confidence: 99%

Novel organization of the site‐specific integration and excision recombination functions of the Staphylococcus aureus serotype F virulence‐converting phages φ13 and φ42

Carroll

Kehoe

Cavanagh

et al. 1995

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…Certain traits, such as the genetic determinants for fibronectin binding protein A (fnbA), clumping factor (clfA), and alpha-hemolysin (hla), were present in all strains tested regardless of SAL, suggesting an important role for these conserved elements in the survival of S. aureus. Other traits, such as tst, cna, and hlb, are known to be associated with mobile genetic elements and were found in this study to be associated with certain lineages and not with others, suggesting limited horizontal transfer among lineages (9,10,14,25,26). For example, cna was present in almost all SAL 1, SAL 5, and SAL 6 isolates but was completely absent from SAL 2 and SAL 4 isolates.…”

Section: Discussionmentioning

confidence: 49%

Clonal Associations among Staphylococcus aureus Isolates from Various Sites of Infection

et al. 2001

View full text Add to dashboard Cite

A molecular epidemiological analysis was undertaken to identify lineages of Staphylococcus aureus that may be disproportionately associated with infection. Pulsed-field gel electrophoresis analysis of 405 S. aureus clinical isolates collected from various infection types and geographic locations was performed. Five distinct S. aureus lineages (SALs 1, 2, 4, 5, and 6) were identified, which accounted for 19.01, 9.14, 22.72, 10.12, and 4.69% of isolates, respectively. In addition, 85 lineages which occurred with frequencies of <2.5% were identified and were termed "sporadic." The most prevalent lineage was methicillin-resistant S. aureus (SAL 4). The second most prevalent lineage, SAL 1, was also isolated at a high frequency from the anterior nares of healthy volunteers, suggesting that its prevalence among clinical isolates may be a consequence of high carriage rates in humans. Gene-specific PCR was carried out to detect genes for a number of staphylococcal virulence traits. tst and cna were found to be significantly associated with prevalent lineages compared to sporadic lineages. When specific infection sites were examined, SAL 4 was significantly associated with respiratory tract infection, while SAL 2 was enriched among blood isolates. SAL 1 and SAL 5 were clonally related to SALs shown by others to be widespread in the clinical isolate population. We conclude from this study that at least five phylogenetic lineages of S. aureus are highly prevalent and widely distributed among clinical isolates. The traits that confer on these lineages a propensity to infect may suggest novel approaches to antistaphylococcal therapy.

show abstract

“…Some Staphylococcus species, such as SAL, are pathogenic and their lipases are involved in the pathogenic processes (Fouace & Ferre, 1970; Fritsche, 1971; Bowden et al , 2002). Up to now, lipase genes have been identified and sequenced for S. hyicus (Götz et al , 1985), S. aureus NCTC8530 (Nikoleit et al , 1995), S. aureus PS54 (Lee & Iandolo, 1985, 1986), S. epidermidis 9 (two lipase genes), S. epidermidis RP62A (Farrell et al , 1993; Simons et al , 1998b; Longshaw et al , 2000; Rosenstein & Götz, 2000), S. haemolyticus (Oh et al , 1999), S. simulans (Sayari et al , 2001a) and S. xylosus (Mosbah et al , 2005). The deduced amino acid sequences of the mature lipases are highly homologous, with amino acid identities ranging from 48% to 99%.…”

Section: Introductionmentioning

confidence: 99%

3-D structure modelling of theStaphylococcus simulanslipase: conformational changes, substrate specificity and novel structural features

Frikha¹,

Ladjimi²,

Gargouri³

et al. 2008

FEMS Microbiology Letters

View full text Add to dashboard Cite

We have modelled, using the CHARMM27 energy force field, the structures of closed and open forms of Staphylococcus simulans lipase (SSL) on the basis of the crystal structures of Bacillus stearothermophilus and Staphylococcus hyicus lipases, respectively. The models suggested the presence of a main lid and a second lid that may act with the former as a double door to control the access to the active site. Superimposition of both closed and open forms of SSL allowed us to determine the hinge regions allowing the movements of the main and the second lid upon lipase activation. The flexibility of these hinge regions was checked by molecular dynamics simulations. The SSL models also allowed us to identify key residues involved in binding substrates, calcium or zinc ions.

show abstract

Mechanism of bacteriophage conversion of lipase activity in Staphylococcus aureus

Cited by 51 publications

References 19 publications

Novel organization of the site‐specific integration and excision recombination functions of the Staphylococcus aureus serotype F virulence‐converting phages φ13 and φ42

Novel organization of the site‐specific integration and excision recombination functions of the Staphylococcus aureus serotype F virulence‐converting phages φ13 and φ42

Clonal Associations among Staphylococcus aureus Isolates from Various Sites of Infection

3-D structure modelling of theStaphylococcus simulanslipase: conformational changes, substrate specificity and novel structural features

Contact Info

Product

Resources

About