Mechanism of drug resistance, characterization of plasmid-borne determinants and transformation study in P. aeruginosa from burn and ICU units-its susceptibility pattern

Shanthi, J.; Pazhanimurugan, Raasaiyah; Gopikrishnan, V.; Balagurunathan, Ramasamy

doi:10.1016/j.burns.2012.08.012

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…P. aeruginosa possesses a considerable degree of natural resistance to many antibiotics. This intrinsic resistance is due to low permeability of its outer membrane, antibiotics such as aminoglycosides, cephalosporins, fluoroquinolones and penicillins are recently non-sensitive to P. aeruginosa [17] . The growing threat of antimicrobial resistance in P. aeruginosa results from the extraordinary capacity of this microorganism for developing resistance to almost any available antibiotic by the selection of mutations in chromosomal genes and from the increasing prevalence of transferrable resistance determinants, particularly those encoding class B carbapenemases (or metallo-β-lactamases [MBLs]) or extended-spectrum β-lactamases (ESBLs), frequently cotransferred with genes encoding aminoglycoside-modifying enzymes [18] , [19] .…”

Section: Discussionmentioning

confidence: 99%

Surveillance and Correlation of Antimicrobial Usage and Resistance of Pseudomonas aeruginosa: A Hospital Population-Based Study

Duan

et al. 2013

PLoS ONE

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This retrospective study evaluated trends and association between resistance of Pseudomonas aeruginosa isolated from patients with hospital-acquired infections (HAIs) and hospital antimicrobial usage from 2003 through 2011 in a tertiary care hospital in northeast China. HAI was defined as occurrence of infection after hospital admission, without evidence that infection was present or incubating (≦48 h) on admission. In vitro susceptibilities were determined by disk diffusion test and susceptibility profiles were determined using zone diameter interpretive criteria, as recommended by Clinical and Laboratory Standards Institute (CLSI). Data on usage of various antimicrobial agents, expressed as defined daily dose (DDD) per 1,000 patients-days developed by WHO Anatomical Therapeutical Chemical (ATC)/DDD index 2011, were collected from hospital pharmacy computer database. Most of 747 strains of P. aeruginosa were collected from respiratory samples (201 isolates, 26.9%), blood (179, 24.0%), secretions and pus (145, 19.4%) over the years. Time series analysis demonstrated a significant increase in resistance rates of P. aeruginosa to ticarcillin/clavulanic acid, piperacillin/tazobactam, cefoperazone/sulbactam, piperacillin, imipenem, meropenem, ceftazidime, cefepime, ciprofloxacin, and levofloxacin except aminoglycosides over time in the hospital (P<0.001). The rates of carbapenem-resistant P. aeruginosa (CRPA) isolated from patients with HAIs were 14.3%, 17.1%, 21.1%, 24.6%, 37.0%, 48.8%, 56.4%, 51.2%, and 54.1% over time. A significant increase in usage of anti-pseudomonal carbapenems (P<0.001) was seen. ARIMA models demonstrated that anti-pseudomonal carbapenems usage was strongly correlated with the prevalence of imipenem and meropenem-resistant P. aeruginosa (P<0.001). Increasing of quarterly CRPA was strongly correlated at one time lag with quarterly use of anti-pseudomonal carbapenems (P<0.001). Our data demonstrated positive correlation between anti-pseudomonal antimicrobial usage and P. aeruginosa resistance to several classes of antibiotics, but not all antimicrobial agents in the hospital.

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Section: Discussionmentioning

confidence: 99%

Surveillance and Correlation of Antimicrobial Usage and Resistance of Pseudomonas aeruginosa: A Hospital Population-Based Study

Duan

et al. 2013

PLoS ONE

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“…This microorganism is known for its ability to adhere to both biological cell membranes and inert surfaces, mediated through the pili and by the production of large amounts of exopolysaccharides (Pier et al, 1987). In addition, this microorganism has strong capability to develop multidrug resistance to many drugs (Ackerman et al, 2013;Shanthi et al, 2013). Additionally, Tredget et al (2004) demonstrated that P. aeruginosa clinical isolates, belonging to the same clone, were present in both the patients and the sink trap.…”

Section: Discussionmentioning

confidence: 99%

Balneotherapy is a potential risk factor for Pseudomonas aeruginosa colonization

Deutsch

Bokehi

Silva

et al. 2016

Braz. J. Pharm. Sci.

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The practice of immersion in burn patient has been abandoned in many parts of the world but in Brazil it is still common. The aim of this study was to ascertain if balneotherapy is a risk factor for Pseudomonas aeruginosa colonization in thermally injured patients. Eighteen patients from a Burn Center were studied for 14 weeks for Pseudomonas aeruginosa. Samples were collected by swabbing the exudate of wounds, before and after giving bath to the patients and from balneotherapy table. Pulsed-field gel electrophoresis was used to determine bacterial genetic relatedness. Thirty-seven P. aeruginosa isolates were detected from 292 swabs collected from patients' burn surface area and from the balneotherapy table. Profile analysis of P. aeruginosa DNA fragmentation showed 10 clones among the 37 strains analyzed. Type A is the most prevalent clone, with 23 strains distributed into eight subtypes. These were present in the swabs collected, before and after the patients' bath, from the surface of the bath table, suggesting that there was cross-contamination between the patients in different ways. This work demonstrates that balneotherapy is a risk factor in the Burn Center studied, because the same clone was found among P. aeruginosa isolates collected at various points and times.Uniterms: Pseudomonas aeruginosa/colonization. Burn patients. Balneotherapy. PFGE.A prática de balneotarapia em paciente queimado foi abandonada em muitas partes do mundo, mas no Brasil ainda é comum. O objetivo deste estudo foi verificar se a balneoterapia é um fator de risco para a colonização por Pseudomonas aeruginosa em pacientes queimados. Dezoito pacientes internados em um Centro de Queimadura (CQ) foram acompanhados por 14 semanas. Amostras foram coletadas do exsudato de feridas, antes e depois do banho dos pacientes e também da mesa onde a balneoterapia foi realizada. A relação genética entre as cepas de P. aeruginosa foi determinada pela electroforese em gel de campo pulsado. Trinta e sete cepas foram detectadas a partir de 292 swabs coletados de área de superfície das feridas dos pacientes e da mesa de balneoterapia. Análise de fragmentação do DNA das 37 P. aeruginosa mostrou a existência de 10 clones. O tipo A foi o clone mais prevalente, com 23 cepas distribuídas em oito subtipos. Estas estavam presentes nas lesões dos pacientes antes e após o banho e na mesa onde o banho foi realizado, sugerindo contaminação cruzada inter e intra-pacientes e pacientes e mesa de banho. Este trabalho mostra que a balneoterapia é um fator de risco para colonização por P. aeruginosa, no CQ estudado, pois um mesmo clone da bactéria foi encontrado nos isolados coletados em vários pontos e épocas diferentes. Unitermos:Pseudomonas aeruginosa/colonização. Pacientes queimados/tratamento. Balneoterapia. Eletroforese em campo pulsado.

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“…Makrolidler, tetrasiklin, rifampin ve sefiksime karşı ise yüksek oranda direnç gösterir. Hızlı direnç gelişiminde rol oynayan mekanizmalar arasında beta-laktamaz üretimi, dış membran geçirgenliğinde azalma ve aktif pompalama sistemleri sayılabilir [3][4][5]. P. aeruginosa çoklu antibiyotik direnci gösterebilmesi ve kullanılan antimikrobiyal ajanlara karşı hızlı direnç geliştirebilmesi nedeniyle özelikle yoğun bakımda izlenen hastaların tedavilerinde sorunlara neden olmaktadır [6,7].…”

Section: Introductionunclassified

“…Çalışmamızda hastanemizde son beş yıl içerisinde çeşitli yoğun bakım ünitelerinden gönderilen örneklerde üretilen P. aeruginosa suşlarının antibiyotik direnç profilinin belirlenmesi amaçlanmıştır. [3,5]. Kritik hastaların hastanede yatış süresinin uzaması, aynı grup antibiyotiğin sürekli kullanımı ve dirençli kolonilerin seçilmesi ise, tedavi başarısızlığına neden olan diğer faktörler olarak göze çarpmaktadır [7,9].…”

Section: Introductionunclassified

Assessment of <i>Pseudomonas aeruginosa</i> resistance profiles in intensive care units: Five-year outcomes

et al. 2017

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Pseudomonas cinsi bakteriler doğada yaygın olarak bulunan, fırsatçı enfeksiyonlar ve hastane enfeksiyonlarına yol açan non-fermantatif Gram negatif basillerdir. Hastanelerde özellikle yoğun bakımlarda izole edilen bu etken mekanik ventilatöre bağlı, kemoterapi ve antibiyotik tedavisi alan bağışıklık sistemi baskılanmış hastalarda önemli morbidite ve mortalite nedenidir. Bu çalışmanın amacı yoğun bakım ünitelerinden izole edilen Pseudomonas aeruginosa (P.aeruginosa) suşlarında antibiyotik duyarlılık profilinin belirlenmesidir.

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Mechanism of drug resistance, characterization of plasmid-borne determinants and transformation study in P. aeruginosa from burn and ICU units-its susceptibility pattern

Cited by 4 publications

References 25 publications

Surveillance and Correlation of Antimicrobial Usage and Resistance of Pseudomonas aeruginosa: A Hospital Population-Based Study

Surveillance and Correlation of Antimicrobial Usage and Resistance of Pseudomonas aeruginosa: A Hospital Population-Based Study

Balneotherapy is a potential risk factor for Pseudomonas aeruginosa colonization

Assessment of <i>Pseudomonas aeruginosa</i> resistance profiles in intensive care units: Five-year outcomes

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