Mechanism of gastrodin in cell apoptosis in rat hippocampus tissue induced by desflurane

Abstract

This study investigated the protective effect ofgastrodin on cell apoptosis in rats hippocampus tissues induced by desflurane to explore its mechanism. A total of 36 rats were randomly divided into three groups: Blank control group (C group, n=12), desflurane anesthesia group (DF group, n=12) and gastrodin treatment group (GT group, n=12). Rats in DF group were treated with anesthesia using desflurane. Rats in GT group were treated with gavage using gastrodin and the same treatment as DF group. After the experiment, novel object recognition test and water maze test were performed. The hippocampus tissues were taken from the rat after the behavioral experiment; then the number of apoptotic cells was detected using the terminal deoxynucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) kit, and the mRNA and protein expression levels of p38 and interleukin-1 (IL-1) were detected via semi-quantitative polymerase chain reaction (PCR) and western blot analysis. After the desflurane anesthesia, novel object recognition showed that compared with that in DF group, the exploration capacity of novel objects in GT group was increased (P<0.01). The water maze test showed that the escape latencies in DF group, T1 in GT group was significantly shortened, but T2 was significantly prolonged (P<0.01). TUNEL assay showed that the number of apoptotic cells in hippocampus tissues in GT group was significantly fewer than that in group DF (P<0.01). Semi-quantitative PCR and western blot analysis showed that the expression levels of p38 and IL-1β in GT group were lower than those in DF group (P<0.01). The results show that gastrodin has a protective effect on the apoptosis of hippocampus cells of rats induced by desflurane. Its protection mechanism may be realized through decreasing the increased p38 and IL-1β expression levels induced by desflurane, thus blocking the p38 mitogen-activated protein kinase (p38 MAPK) pathway.

Gastrodin improves preeclampsia‐induced cell apoptosis by regulation of TLR4/NF‐κB in rats

“…Gastrodia elata, a precious treasure in traditional Chinese medicine, has been proven to be effective in the treatment of various diseases with a favorable safety profile. Gastrodin is the bioactive component of Gastrodia elata and shows high activities in sedation, blood supply improvement, anti‐inflammation, blood vessel function regulation, and neuroprotection (Liu et al, ; Wang, Wang, & Duan, ). Studies have demonstrated that placental trophoblasts in patients with preeclampsia undergo excessive apoptosis and the degree of apoptosis is related to the severity of the disease (Heazell, Buttle, & Baker, ).…”

“…By cellular immunofluorescence, the p-NF-κB (p65) was expressed in cytoplasm, the p-NF-κB (p65) protein expressions of Model group were significantly increased compared with those of NC group (p < .001, respectively, Figure 6); with gastrodin supplement, the p-NF-κB (p65) protein expressions of gastrodin-treated groups were significantly depressed compared with those of model group (p < .05, respectively, Figure 6). neuroprotection (Liu et al, 2018;Wang, Wang, & Duan, 2018). Studies have demonstrated that placental trophoblasts in patients with preeclampsia undergo excessive apoptosis and the degree of apoptosis is related to the severity of the disease (Heazell, Buttle, & Baker, 2008).…”

The negative effect of desflurane on reproductive capacity in female rats

“…In addition, desfl urane has been shown to induce the apoptosis mechanism, especially in neuron cells, and causes accelerated cell death. Cysteine-containing aspartate-specifi c proteases 3 (Caspase 3) and the nuclear factor-kappaB (NF-kappaB)-dependent apoptosis pathway has a very important role in the death of neurons (12,21,22). NF-kappaB is an important regulator of genes that control apoptosis.…”

“…No: YHB0616Ra; Shanghai; China). The serum IL-1β levels were duly measured according to literature (12). At the end of the procedure, the absorbance was measured with a spectrophotometer at a wavelength of 450 nm via a microplate reader.…”

Rapid Eye Movement Sleep Deprivation Combined With Fluoxetine Protects Against Depression-Induced Damage and Apoptosis in Rat Hippocampi via A1 Adenosine Receptor

“…Terminal transferase-mediated biotin 2 ′ -deoxyuridine, 5 ′triphosphate nick end labeling (TUNEL) assay was performed to evaluate apoptosis of cells with a TUNEL assay kit (ab206386, Abcam, Cambridge, UK) as previously described (27). The hippocampus tissue was first deparaffinized and treated with 20 µg/ml deoxyribonuclease-free proteinase K (AM2548, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 20 min and was then washed with PBS three times (5 min for each).…”