Mechanism of inhibition and decoupling of oxygen evolution from electron transfer in photosystem II by fluoride, ammonia and acetate

Lovyagina, E.R.; Semin, B.K.

doi:10.1016/j.jphotobiol.2016.02.031

Cited by 16 publications

(10 citation statements)

References 68 publications

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“…It is important to especially note that the decrease in the F M value caused by both [CuL 2 ]Br 2 concentrations is at list in ten times more significant than the increase in the F 0 value induced by [CuL 2 ]Br 2 without DCMU. An increase in the F 0 level in the presence of DCMU has been repeatedly noted earlier on leaves [40], thylakoids [41], and PSII-containing membranes [42][43][44]. Both types of these changes (F M and F 0 levels) lead to a decrease in F V /F M ratio.…”

Section: Solutions Of Inhibitory Agentssupporting

confidence: 68%

“…Kinetics measured in the absence of additions (control) are completely identical to those recorded on PSII-containing membranes [42][43][44]49]. There is no peak I in the kinetic (plateau J-I), the main feature characterizing the kinetics of fast chlorophyll fluorescence induction measured on PSII-containing membranes [42][43][44]49] and therefore the kinetics will be designated below as OJP kinetics [42]. The absence of peak I (plateau J-I) in the OJP kinetics of PSII-containing membranes has been substantiated previously [42].…”

Section: Original Ojip Kinetics Normalized Relative To F 0 (F 002ms )mentioning

confidence: 53%

“…The analysis of the presented kinetics shows the following main properties of the obtained kinetics and their changes caused by the studied agents and their combinations. Kinetics measured in the absence of additions (control) are completely identical to those recorded on PSII-containing membranes [42][43][44]49]. There is no peak I in the kinetic (plateau J-I), the main feature characterizing the kinetics of fast chlorophyll fluorescence induction measured on PSII-containing membranes [42][43][44]49] and therefore the kinetics will be designated below as OJP kinetics [42].…”

Section: Original Ojip Kinetics Normalized Relative To F 0 (F 002ms )mentioning

confidence: 73%

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Probing the Influence of Novel Organometallic Copper(II) Complexes on Spinach PSII Photochemistry Using OJIP Fluorescence Transient Measurements

Zharmukhamedov,

Shabanova,

Huseynova

et al. 2023

Biomolecules

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Modern agricultural cultivation relies heavily on genetically modified plants that survive after exposure to herbicides that kill weeds. Despite this biotechnology, there is a growing need for new sustainable, environmentally friendly, and biodegradable herbicides. We developed a novel [CuL2]Br2 complex (L = bis{4H-1,3,5-triazino[2,1-b]benzothiazole-2-amine,4-(2-imidazole) that is active on PSII by inhibiting photosynthetic oxygen evolution on the micromolar level. [CuL2]Br2 reduces the FV of PSII fluorescence. Artificial electron donors do not rescind the effect of [CuL2]Br2. The inhibitory mechanism of [CuL2]Br2 remains unclear. To explore this mechanism, we investigated the effect of [CuL2]Br2 in the presence/absence of the well-studied inhibitor DCMU on PSII-containing membranes by OJIP Chl fluorescence transient measurements. [CuL2]Br2 has two effects on Chl fluorescence transients: (1) a substantial decrease of the Chl fluorescence intensity throughout the entire kinetics, and (2) an auxiliary “diuron-like” effect. The initial decrease dominates and is observed both with and without DCMU. In contrast, the “diuron-like” effect is small and is observed only without DCMU. We propose that [CuL2]Br2 has two binding sites for PSII with different affinities. At the high-affinity site, [CuL2]Br2 produces effects similar to PSII reaction center inhibition, while at the low-affinity site, [CuL2]Br2 produces effects identical to those of DCMU. These results are compared with other PSII-specific classes of herbicides.

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Section: Solutions Of Inhibitory Agentssupporting

confidence: 68%

Section: Original Ojip Kinetics Normalized Relative To F 0 (F 002ms )mentioning

confidence: 53%

Section: Original Ojip Kinetics Normalized Relative To F 0 (F 002ms )mentioning

confidence: 73%

See 1 more Smart Citation

Probing the Influence of Novel Organometallic Copper(II) Complexes on Spinach PSII Photochemistry Using OJIP Fluorescence Transient Measurements

Zharmukhamedov,

Shabanova,

Huseynova

et al. 2023

Biomolecules

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show abstract

“…The variability of photosynthetic pigments' contents visually reflected the photosynthetic activity of C. sorokiniana. Because of its sensitivity to growth conditions, nutrient availability and various abiotic stresses, PSII efficiency is employed to evaluate the stress response of microalgae ( Lovyagina and Semin, 2016 ). As a rapid and non-invasive technology, chlorophyll fluorescence test is widely used to assess the performance of PSII under different conditions ( Kumar et al., 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal molecular mechanisms for antibiotic removal

et al. 2022

iScience

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“…The NH 4 Cl addition not only inhibited the level of PtLhcx3 transcript, but also reduced the increase of NPQ in HL. NH 4 Cl can remove the ΔpH of both sides of thylakoid membrane directly (Lovyagina and Semin 2016 ) while the induction of NPQ depends on the ΔpH (Eisenstadt et al 2010 ). For now, it is still unclear how ΔpH induces NPQ.…”

Section: Discussionmentioning

confidence: 99%

Light-harvesting protein Lhcx3 is essential for high light acclimation of Phaeodactylum tricornutum

et al. 2018

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The light-harvesting protein complexes (Lhc) play key roles in the processes of light absorption and protection in diatoms. However, different Lhc protein carries out distinct function in photosynthesis. For now, roles of many Lhc proteins in light acclimation are largely unknown. Here, function of Lhcx3 in marine diatom Phaeodactylum tricornutum was examined by using reverse genetic technologies. The overexpression of Lhcx3 led to increased diadinoxanthin + diatoxanthin content and elevated non-photochemical fluorescence quenching (NPQ) while knockdown of Lhcx3 reduced NPQ level. In addition, the expression of Lhcx3 could be induced by blue light but not by red light. After addition of the photosynthetic inhibitor, upregulation of Lhcx3 transcript in high light could be inhibited by NH4Cl, but not by DCMU (3-(3,4-dichlorophenyl)-l,l-dim ethylurea). In contrast, DCMU addition increased expression of Lhcx3 in high light. In combination with changes of NPQ after addition of inhibitor, we concluded that the Lhcx3 played key roles in high light acclimation of diatoms. This finding will provide new clues for genetic improvement of P. tricornutum with an aim to cultivate new strains with high growth rate.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0703-3) contains supplementary material, which is available to authorized users.

show abstract

Mechanism of inhibition and decoupling of oxygen evolution from electron transfer in photosystem II by fluoride, ammonia and acetate

Cited by 16 publications

References 68 publications

Probing the Influence of Novel Organometallic Copper(II) Complexes on Spinach PSII Photochemistry Using OJIP Fluorescence Transient Measurements

Probing the Influence of Novel Organometallic Copper(II) Complexes on Spinach PSII Photochemistry Using OJIP Fluorescence Transient Measurements

Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal molecular mechanisms for antibiotic removal

Light-harvesting protein Lhcx3 is essential for high light acclimation of Phaeodactylum tricornutum

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