MECHANISM OF PROSTAGLANDIN BIOSYNTHESIS ¹⁸O₂ STUDIES ON PGF_1α

Inhibition of Prostaglandin Biosynthesis by Corticosteroids

“…The skin was weighed and cut into small fragments with scissors before immersion in phosphate buffer (pH 7A4) containing hydroquinone (0-275 mM) and glutathione (0-325 mM) (Foss et al, 1971). After homogenization with a Polytron tissue homogenizer bovine serum albumin was added (final concentration 5 mg/ml).…”

Formation of an Intermediate in Prostaglandin Biosynthesis and Its Association with the Platelet Release Reaction

“…In crude extracts from biological sources, this method would also measure 11-dehydro-PGF2a, which is almost exclusively converted to PGF2< by sodium borohydride reduction (1). However, 11-dehydro-PGF2, (PGD2) elutes before PGE2 from silicic acid columns (16) and therefore should not interfere in our estimations of PGE2 formation. This assumption was confirmed by the finding that radioimmunoassay and bioassay gave essentially the same values for PGE2 formation (cf.…”

Anti-Inflammatory Activity of Piperonyl-4-Acrylic Isobutyl Amide, an Extractive from Zanthoxylum zanthoxyloides

“…Guinea pig lung homogenate preparation: Lungs from 5 adult guinea pigs (of either sex, 350 g and above) were pooled together in each experiment. The lungs were homogenized in an MSE blade homogenizer [5] in cold phosphate buffer (pH = 7.4) containing 0.27 nM hydroquinone and 0.325 mM reduced glutathione [6] in a tissue: medium ratio of 1:4. The resultant suspension was further homogenized in a Potter-Elvehjem homogenizer by six up and down strokes of the teflon pestle.…”