Mechanism of resistance and antibacterial susceptibility in extended-spectrum β-lactamase phenotype Klebsiella pneumoniae and Klebsiella oxytoca isolated between 2000 and 2010 in Japan

Abstract

Clinical isolates of Klebsiella pneumoniae and Klebsiella oxytoca collected from 20 Japanese medical facilities between 2000 and 2010 were analysed to evaluate the mechanisms of resistance and antibacterial susceptibilities to 14 antimicrobials. Overall, eight of 484 (1.6 %) K. pneumoniae and 19 of 359 (5.3 %) K. oxytoca were determined to be extendedspectrum b-lactamase (ESBL) phenotype isolates, and the identified ESBLs amongst the K. pneumoniae isolates were CTX-M-2, -3, -14 and -15, and SHV-12. In contrast, overproduction of chromosomal b-lactamase OXY-2, which was due to a distinct mutation at the 210 promoter region of this gene, conferred the ESBL phenotype to all the K. oxytoca isolates except one. Based on the Clinical and Laboratory Standards Institute breakpoints, all the ESBL phenotype K. pneumoniae were susceptible to doripenem, flomoxef, moxalactam (latamoxef), cefmetazole and tazobactam/piperacillin, whereas the ESBL phenotype K. oxytoca were susceptible to ceftazidime and ceftibuten in addition to the above, with the exception of tazobactam/piperacillin. Amongst the oral antimicrobials, ceftibuten was relatively effective against both ESBL phenotype Klebsiella species compared with levofloxacin and amoxicillin/ clavulanic acid.

Phenotypic and Molecular Characterization of Antimicrobial Resistance in Klebsiella spp. Isolates from Companion Animals in Japan: Clonal Dissemination of Multidrug-Resistant Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae

“…We found higher prevalence of ESBLs in KP clinical isolates (31/89, 34.8%), compared with those from companion animals in Italy (15/70, 21.4%, Donati et al, 2014 ), and in Germany and other European countries (84/1112, 7.6%, Ewers et al, 2014 ). In addition, this carriage rate of ESBLs was extremely higher than that in human isolates in Japan (8/484, 1.6%, Sato et al, 2015 ). These data suggest that the risk of ESBL carriage is relatively high in KP clinical isolates from companion animals in Japan.…”

Update of incidence and antimicrobial susceptibility trends of Escherichia coli and Klebsiella pneumoniae isolates from Chinese intra-abdominal infection patients

“…Although a significant increase of the proportion of ESBL-positive Enterobacteriaceae hospital infections in Germany over the period 2007–2012 [ 9 ] and in Japan from 2000 to 2010 have been reported [ 10 , 11 ] the situation in China is not clear. A limited number of ESBL-screen positive Escherichia coli and Klebsiella pneumoniae IAI isolates from 2012 and 2013 have been documented, but there is a wide diversity in ESBL-related molecular characteristics [ 12 ].…”

bla SHV-12 gene detection from Klebsiella pneumoniae producing Extended-Spectrum β-Lactamase using amplification-refractory mutation system method

“…Directions correlation is negative (-) indicates that the more samples showed positive bla SHV-12 gene, the less it resistant to these antibiotics. Based on one research, all the ESBL phenotype K. pneumoniae were susceptible to some antibiotics including cefmetazole [24]. Also in other research, all ESBL producers were susceptible to imipenem [25].…”