Mechanism of resistance to spinosyn in the tobacco budworm, Heliothis virescens

Roe, R. Michael; Young, Hugh P.; Iwasa, Takao; Wyss, C. F.; Stumpf, Christof; Sparks, Thomas C.; Watson, Gerald B.; Sheets, Joel J.; Thompson, Gary D.

doi:10.1016/j.pestbp.2009.08.009

Cited by 25 publications

(14 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…133 A study of cross-resistance with spinosyn in tobacco budworm has been recently published. 134 Efficacy of spinosad against cat fleas on dogs was also reported. 135 …”

Section: Resultsmentioning

confidence: 98%

The spinosyn family of insecticides: realizing the potential of natural products research

Kirst¹

2010

J Antibiot

288

163

View full text Add to dashboard Cite

The spinosyns are a large family of unprecedented compounds produced from fermentation of two species of Saccharopolyspora. Their core structure is a polyketide-derived tetracyclic macrolide appended with two saccharides. They show potent insecticidal activities against many commercially significant species that cause extensive damage to crops and other plants. They also show activity against important external parasites of livestock, companion animals and humans. Spinosad is a defined combination of the two principal fermentation factors, spinosyns A and D. Structure-activity relationships (SARs) have been extensively studied, leading to development of a semisynthetic second-generation derivative, spinetoram. The spinosyns have a unique mechanism of action (MOA) involving disruption of nicotinic acetylcholine receptors. When compared with many other insecticides, the spinosyns generally show greater selectivity toward target insects and lesser activity against many beneficial predators as well as mammals and other aquatic and avian animals. Their insecticidal spectrum, unique MOA and lower environmental effect make them useful new agents for modern integrated pest management programs. As a result, this work has received U S Presidential Green Chemistry Challenge Awards.

show abstract

“…133 A study of cross-resistance with spinosyn in tobacco budworm has been recently published. 134 Efficacy of spinosad against cat fleas on dogs was also reported. 135 …”

Section: Resultsmentioning

confidence: 98%

The spinosyn family of insecticides: realizing the potential of natural products research

Kirst¹

2010

J Antibiot

288

163

View full text Add to dashboard Cite

show abstract

“…In such species, there is evidence of resistance being a result of either enhanced metabolism (Markussen and Kristensen ) or a consequence of changes in the target site (Roe et al . ). Studies conducted with the model insect species Drosophila melanogaster have implicated the nAChR Dα6 subunit in determining target‐site resistance to spinosad (Perry et al .…”

mentioning

confidence: 97%

A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis

Puinean

Lansdell

Collins

et al. 2013

Journal of Neurochemistry

111

103

View full text Add to dashboard Cite

High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis, an economically important insect pest of vegetables, fruit and ornamental crops. We have cloned the nicotinic acetylcholine receptor (nAChR) α6 subunit from F. occidentalis (Foα6) and compared the nucleotide sequence of Foα6 from susceptible and spinosad-resistant insect populations (MLFOM and R1S respectively). A single nucleotide change has been identified in Foα6, resulting in the replacement of a glycine (G) residue in susceptible insects with a glutamic acid (E) in resistant insects. The resistance-associated mutation (G275E) is predicted to lie at the top of the third α-helical transmembrane domain of Foα6. Although there is no direct evidence identifying the location of the spinosad binding site, the analogous amino acid in the C. elegans glutamate-gated chloride channel lies in close proximity (4.4 Å) to the known binding site of ivermectin, another macrocyclic lactone pesticide. The functional consequences of the resistance-associated mutation have been examined in the human nAChR α7 subunit. Introduction of an analogous (A272E) mutation in α7 abolishes the modulatory effects of spinosad whilst having no significant effect upon activation by acetylcholine, consistent with spinosad having an allosteric mechanism of action.

show abstract

“…A study of cross resistance with spinosad in tobacco bud worm has been recently reported by Roe (2010). The estimated decrease in resistance was 11.36, 11.11, 16.67 and 9.61 for imidacloprid, spinosad, emamectin benzoate and profenofos, respectively and suggested that spinosad could be included in the control program of S. litura, due to its lower stability and higher reversion rate with insecticides bearing novel modes of action (Adeel et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Negative Cross Resistance of Leucinodes orbonalis Population of Brinjal to Newer Molecule Spinetoram 12 SC W/V (11.7 W/W)

Visnupriya¹,

Muthukrishnan²

2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Mechanism of resistance to spinosyn in the tobacco budworm, Heliothis virescens

Cited by 25 publications

References 18 publications

The spinosyn family of insecticides: realizing the potential of natural products research

The spinosyn family of insecticides: realizing the potential of natural products research

A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis

Negative Cross Resistance of Leucinodes orbonalis Population of Brinjal to Newer Molecule Spinetoram 12 SC W/V (11.7 W/W)

Contact Info

Product

Resources

About