Mechanism of the melanogenesis stimulation activity of (−)-cubebin in murine B16 melanoma cells

Hirata, Noriko; Naruto, Shunsuke; Ohguchi, Ken-ichi; Akao, Yukihiro; Nozawa, Yoshinori; Iinuma, Munekazu; Matsuda, Hideaki

doi:10.1016/j.bmc.2007.04.046

Cited by 72 publications

(63 citation statements)

References 13 publications

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“…MAPKs signaling pathway is reported to be responsible for cell proliferation and differentiation of melanoma (Wong, et al, 1974;Landa, et al, 1998;Hirata, et al, 2007). To determine whether MAPKs pathway was involved in CMSEE-induced differentiation of B16 F1 melanoma cells.…”

Section: Effects Of Cmsee On the Activation Of Mapks Pathwaymentioning

confidence: 99%

An Ester Extract of Cochinchina Momordica Seeds Induces Differentiation of Melanoma B16 F1 Cells via MAPKs Signaling

Zhao

Han²,

Ren³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Section: Effects Of Cmsee On the Activation Of Mapks Pathwaymentioning

confidence: 99%

An Ester Extract of Cochinchina Momordica Seeds Induces Differentiation of Melanoma B16 F1 Cells via MAPKs Signaling

Zhao

Han²,

Ren³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…Furthermore, it has been shown that extracts from some herbs, including kava rhizome extract (43), glycyrrhizin (44), lotus flower (45), cubebin (46), quercetin (47) and naringenin (17) increase melanogenesis in B16 melanoma cells and human melanocytes, thereby indicating that natural resources should be extensively screened for the development of gray hair prevention agents or therapeutic drugs to induce repigmentation in the skin of vitiligo patients.…”

Section: Discussionmentioning

confidence: 99%

Mangosteen leaf extract increases melanogenesis in B16F1 melanoma cells by stimulating tyrosinase activity in vitro and by up-regulating tyrosinase gene expression

2011

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Abstract. Melanin synthesis is stimulated by various effectors, including α-melanocyte stimulating hormone (α-MSH), cyclic AMP (cAMP)-elevating agents (forskolin, isobutylmethylxantine, glycyrrhizin) and ultraviolet light. Our investigation focused on the identification of the melanogenic efficacy of mangosteen (Garcinia mangostana) leaf extract with regard to its effects on melanogenesis in B16F1 melanoma cells, since it has been known to possess strong anti-oxidant activities. The mangosteen leaf extract was found to stimulate melanin synthesis and tyrosinase activity in a dose-dependent manner without any significant effects on cell proliferation. Cytotoxicity of the extract was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; the highest concentration of the extract that did not affect cell viability was 32 µg/ml. Formation of melanin from cultured B16F1 melanoma induced by extract treatment was estimated using spectrophotometry. In order to clarify the subsequent mechanism of tyrosinase activation by the extract, the levels of tyrosinase expression in B16F1 melanoma were examined using an intracellular tyrosinase assay and tyrosinase zymography. Up-regulation of intracellular tyrosinase expression seemed to correlate with an increase in microphtalmia-associated transcription factor (MITF) protein levels since MITF is the key factor for genes involved in melanogenesis. Both of the results showed that tyrosinase activity was markedly enhanced from extract-treated cells. The overall results suggest that mangosteen leaf extract may be a promising candidate for the treatment of hypopigmentation disorder and useful for selftanning cosmetic products.

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“…Especially, (-)-cubebin showed the most potent melanogenesis stimulation activity in B16 melanoma cells without any significant effects on cell proliferation. Since (-)-cubebin is a new melanogenesis stimulating substance, we tried to elucidate its melanogenesis stimulation mechanism by using B16 melanoma cells (Hirata et al, 2007). Tyrosinase activity was increased at 24 to 72 h after addition of (-)-cubebin to B16 melanoma cells, and then the intracellular melanin amount was increased at 48 to 96 h after the treatment.…”

Section: Melanin Content (µG/well)mentioning

confidence: 99%

Melanin Hyperpigmentation Inhibitors from Natural Resources

Matsuda¹,

Murata²,

Itoh³

et al. 2011

Advances in Malignant Melanoma - Clinical and Research Perspectives

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Mechanism of the melanogenesis stimulation activity of (−)-cubebin in murine B16 melanoma cells

Cited by 72 publications

References 13 publications

An Ester Extract of Cochinchina Momordica Seeds Induces Differentiation of Melanoma B16 F1 Cells via MAPKs Signaling

An Ester Extract of Cochinchina Momordica Seeds Induces Differentiation of Melanoma B16 F1 Cells via MAPKs Signaling

Mangosteen leaf extract increases melanogenesis in B16F1 melanoma cells by stimulating tyrosinase activity in vitro and by up-regulating tyrosinase gene expression

Melanin Hyperpigmentation Inhibitors from Natural Resources

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