Mechanism of YLTZ on glycolipid metabolism based on UPLC/TOF/MS metabolomics

Zhuo, Juncheng; Cai, Dake; Xie, Kaifeng; Gan, Haining; Li, Shasha; Huang, Xuejun; Huang, Dane; Zhang, Chengzhe; Li, Ruyue; Chen, Yu-xing; Zeng, Xiaohui

doi:10.1016/j.jchromb.2018.08.023

Cited by 14 publications

(5 citation statements)

References 45 publications

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“…Bile acids are steroid acids mainly found in mammalian bile. Bile acid can regulate the digestion and absorption of intestinal and liver lipids, and its transformation is closely related to intestinal flora [ 48 ]. Bile acid involves key enzymes that balance cholesterol in vivo.…”

Section: Discussionmentioning

confidence: 99%

Metabonomic Study on the Plasma of High-Fat Diet-Induced Dyslipidemia Rats Treated with Ge Gen Qin Lian Decoction by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry

Sheng

Zeng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Gegen Qinlian decoction (GGQLD) has a definite effect on T2DM in clinic, and it has the effect of lowering blood sugar, improving insulin resistance, and improving the blood lipid level of rats with dyslipidemia, but the intervention mechanism of GGQLD on dyslipidemia has not been clarified. The changes in endogenous metabolites in the plasma of high-fat diet-induced dyslipidemia rats treated with Ge Gen Qin Lian Decoction (GGQLD) were studied to elucidate the therapeutic effects and mechanism of action of GGQLD in dyslipidemia. Based on ultrahigh-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS), the metabolic profiles of rat serum samples were collected. The rat model of dyslipidemia was induced by a 60% fat-fed high-fat diet. After feeding the rats with a high-fat diet for 4 weeks, dyslipidemia appeared. After 5 weeks of GGQLD (14.85 g kg−1) administration, the metabonomics of rats’ plasma samples in the normal group, model group, and administration group were analyzed. Mass profiler professional (MPP), SIMCA-P 14.1, and Graphpad prism 6.0 software were used combined with METLIN biological database and human metabolite database HMDB to screen and identify endogenous biomarkers. Metaboanalyst 4.0 software was used by combining with HMDB and KEGG databases; the enrichment and metabolic pathway of biomarkers were analyzed to explore the metabolic mechanism of dyslipidemia rats induced by high-fat diet and the intervention mechanism of Gegen Qinlian decoction. After 5 weeks of administration of GGQLD, the levels of serum TC and TG were significantly decreased ( P < 0.05 , P < 0.01 ), while HDL-C and LDL-C were not significantly affected. After administration, the food intake of rats in the administration group decreased gradually, and the change trend of body weight gradually slowed down. The metabonomics of rat plasma samples results showed that 23 potential biomarkers including α-linolenic acid, arachidonic acid, and lysophosphatidylcholine were significantly changed in positive ion mode. Studies have shown that GGQLD has a significant lipid-lowering effect on dyslipidemia rats induced by a high-fat diet, and its preventive mechanism is related to tryptophan metabolism, fatty acid biosynthesis, α-linolenic acid metabolism, arachidonic acid, and glycerophosphatidyl metabolism pathway.

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Section: Discussionmentioning

confidence: 99%

Metabonomic Study on the Plasma of High-Fat Diet-Induced Dyslipidemia Rats Treated with Ge Gen Qin Lian Decoction by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry

Sheng

Zeng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Bile acid involves key enzymes that balance cholesterol in vivo. Bile acid participates in glycolipid and energy metabolism [47] through different pathways. In this experiment, compared with the normal group, the increase of sphingolipid content in plasma and the decrease of bile acid content in the model group suggest that the obstacle of energy consumption, the accumulation of energy accelerate fat synthesis, and promote abnormal blood lipid and weight gain.…”

Section: Sphingolipid and Bile Acid Metabolismmentioning

confidence: 99%

Metabonomic Study on the Plasma of High-Fat Diet-Induced Dyslipidemia Rats Treated With Ge Gen Qin Lian Decoction By Ultra High Performance Liquid Chromatography–Mass Spectrometry

Sheng

Zeng

et al. 2020

Preprint

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Background: Gegen Qinlian decoction(GGQLD) has a definite effect on T2DM in clinic, and it has the effect of lowering blood sugar, improving insulin resistance, and improve the blood lipid level of rats with dyslipidemia, but the intervention mechanism of GGQLD on dyslipidemia has not been clarified. Methods: Based on ultra high performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS), the metabolic profiles of rat serum samples were collected. The rat model of dyslipidemia was induced by 60% fat-fed high-fat diet. After feeding the rats with high-fat diet for 4 weeks, the dyslipidemia appeared. After 5 weeks of GGQLD(14.85g•kg-1) administration, the metabonomics of rats plasma samples in normal group, model group and administration group were analyzed. Mass profiler professional (MPP), SIMCA-P 14.1 and Graphpad prism 6.0 software were used combined with METLIN biological database and human metabolite database HMDB to screen and identify endogenous biomarkers. Metaboanalyst 4.0 software was used by combining with HMDB and KEGG databases, the enrichment and metabolic pathway of biomarkers were analyzed to explore the metabolic mechanism of dyslipidemia rats induced by high-fat diet and the intervention mechanism of Gegen Qinlian decoction.Results: After 5 weeks of administration of GGQLD, the levels of serum TC and TG were significantly decreased (P < 0.05, P < 0.01), while HDL-C and LDL-C were not significantly affected. After administration, the food intake of rats in administration group decreased gradually, and the change trend of body weight gradually slowed down. The metabonomics of rat plasma samples results showed that 23 potential biomarkers including α - linolenic acid, arachidonic acid and lysophosphatidylcholine were significantly changed in positive ion mode.Conclusion: Studies have shown that GGQLD has significant lipid-lowering effect on dyslipidemia rats induced by high-fat diet, and its preventive mechanism is related to tryptophan metabolism, fatty acid biosynthesis, α - linolenic acid metabolism, arachidonic acid, glycerophosphatidyl metabolism pathway.

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“…The experimental results showed that YLTZC remarkably lowered the levels of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-c) in HLP rabbits, mice, and rats, while increasing the levels of high-density lipoprotein cholesterol (HDL-c), and the anti-HLP effect of YLTZC might be related to inhibiting PXR expression, promoting bile acid excretion and RCT processes, and enhancing TG hydrolysis 4 – 7 . In addition, in the treatment of type 2 diabetes mellitus, YLTZC was performed to prohibit the FA β-oxidation, synthesis of cholesterol and phospholipids, gluconeogenesis, and inflammation level, as well as promote TG hydrolysis, glycolysis, and blood circulation 8 . However, there are still some problems in the related research of YLTZC.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the mechanisms of Yinlan Tiaozhi capsule in treating hyperlipidemia by combining network pharmacology, molecular docking and experimental verification

Xiao¹,

Hu²,

Jia

et al. 2023

Sci Rep

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Yinlan Tiaozhi capsule (YLTZC) has been widely used to treat hyperlipidemia (HLP). However, its material basis and underlying pharmacological effects remain unclean. The current study aimed to explore the mechanisms involved in the treatment of YLTZC on HLP based on network pharmacology, molecular docking, and experimental verification. Firstly, UPLC-Q-TOF–MS/MS was used to comprehensively analyze and identify the chemical constituents in YLTZC. A total of 66 compounds, mainly including flavonoids, saponins, coumarins, lactones, organic acids, and limonin were characterized and classified. Simultaneously, the mass fragmentation pattern of different types of representative compounds was further explored. By network pharmacology analysis, naringenin and ferulic acid may be the core constituents. The 52 potential targets of YLTZC, including ALB, IL-6, TNF, and VEGFA, were considered potential therapeutic targets. Molecular docking results showed that the core active constituents of YLTZC (naringenin and ferulic acid) have a strong affinity with the core targets of HLP. Lastly, animal experiments confirmed that naringenin and ferulic acid significantly upregulated the mRNA expression of ALB and downregulated the mRNA expression of IL-6, TNF, and VEGFA. In sum, the constituents of YLTZC, such as naringenin and ferulic acid, might treat HLP by regulating the mechanism of angiogenesis and inhibiting inflammatory responses. Furthermore, our data fills the gap in the material basis of YLTZC.

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Mechanism of YLTZ on glycolipid metabolism based on UPLC/TOF/MS metabolomics

Cited by 14 publications

References 45 publications

Metabonomic Study on the Plasma of High-Fat Diet-Induced Dyslipidemia Rats Treated with Ge Gen Qin Lian Decoction by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry

Metabonomic Study on the Plasma of High-Fat Diet-Induced Dyslipidemia Rats Treated with Ge Gen Qin Lian Decoction by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry

Metabonomic Study on the Plasma of High-Fat Diet-Induced Dyslipidemia Rats Treated With Ge Gen Qin Lian Decoction By Ultra High Performance Liquid Chromatography–Mass Spectrometry

Deciphering the mechanisms of Yinlan Tiaozhi capsule in treating hyperlipidemia by combining network pharmacology, molecular docking and experimental verification

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