Mechanisms and efficacy of traditional Chinese herb monomers in diabetic kidney disease

Chen, Xuexun; Li, Xuan; Chen, Bo; Chen, Xinping; Zhang, Kexin; Han, Fang; Kan, Chengxia; Zhang, Jingwen; Sun, Xiaodong; Guo, Zhentao

doi:10.1007/s11255-023-03703-0

Cited by 3 publications

(1 citation statement)

References 119 publications

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“…In recent years, the medicinal properties of TCM have revealed its ability to regulate blood glucose and lipid metabolism, mitigate kidney damage, delay kidney disease progression, and prevent glomerulosclerosis and fibrosis. [ 5 ] Given the complex pathogenesis and diverse factors implicated in these diseases, the development paradigm of new drugs has shifted from a “single target, single drug” approach to a “multiple targets, multiple components” strategy. TCM satisfies the requirements for multi-target, multi-component, and individualized treatment while ensuring safety and efficacy.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Huaiqihuang in treatment of diabetic kidney disease based on network pharmacology, molecular docking and in vitro experiment

Wang,

Ma,

Zhang

et al. 2023

Medicine

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Background: This study aimed to investigate the active components, key targets, and potential molecular mechanisms Huaiqihuang (HQH) in the treatment of diabetic kidney disease (DKD) through network pharmacology, molecular docking, and in vitro experiments. Methods: The active components and potential targets of HQH were obtained from the TCMSP and HERB databases. The potential targets of DKD were obtained from the GeneCards, OMIM, DrugBank, and TTD databases. Protein interaction relationships were obtained from the STRING database, and a protein interaction network was constructed using Cytoscape software. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed using the Metascape database. Molecular docking was performed using AutoDock software to verify the binding between key compounds and core target genes. In vitro experiments were conducted using human renal proximal tubular epithelial cells and various methods, such as CCK8, RT-PCR, immunofluorescence, and western blot, to evaluate the effects of HQH on inflammatory factors, key targets, and pathways. Results: A total of 48 active ingredients, 168 potential targets of HQH, and 1073 potential targets of DKD were obtained. A total of 118 potential targets, 438 biological processes, and 187 signal pathways were identified for the treatment of DKD. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that HQH may exert its therapeutic effects on DKD by regulating the expression of inflammatory factors through the nuclear factor kappa B (NF-κB) signaling pathway. The molecular docking results showed that β-sitosterol and baicalein had the highest binding affinity with key targets such as AKT1, IL6, TNF, PTGS2, IL1B, and CASP3, suggesting that they may be the most effective active ingredients of HQH in the treatment of DKD. In vitro experimental results demonstrated that HQH could enhance the viability of human renal proximal tubular epithelial cells inhibited by high glucose, decrease the levels of AKT1, TNF, IL6, PTGS2, IL1B, and CASP3, reduce the expression of NF-κB-P65 (P < .01), inhibit NF-κB-p65 nuclear translocation, and decrease chemokine expression (P < .01). Conclusion: HQH may exert its therapeutic effects on DKD by inhibiting the NF-κB signaling pathway, reducing the level of pro-inflammatory cytokines, and alleviating the high glucose-induced injury of renal tubular epithelial cells.

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Section: Introductionmentioning

confidence: 99%

Mechanism of Huaiqihuang in treatment of diabetic kidney disease based on network pharmacology, molecular docking and in vitro experiment

Wang,

Ma,

Zhang

et al. 2023

Medicine

View full text Add to dashboard Cite

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Chinese herbal medicine for the treatment of diabetic nephropathy: From clinical evidence to potential mechanisms

Gong,

Cao,

et al. 2024

Journal of Ethnopharmacology

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Carrier-Free Small Molecule-Assembled Nanoparticles for Treatment of Sepsis

Xing,

Sheng,

et al. 2024

ACS Appl. Nano Mater.

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Sepsis is a complex disorder with high morality. Overabundant inflammation can result in organ dysfunction, leading to a series of diseases. Drug delivery systems have improved drug efficacy and brought hope for sepsis treatment. However, the usage of various inorganic and organic carriers might increase the potential toxicity of nanoparticles (NPs) and make their clinical transformation more difficult. Rhubarb and epimedium show good anti-inflammation and metabolic disorder treatment potentials. A series of ingredients that can form nanoscale particles without any additives were found in rhubarb and epimedium decoction. Based on pharmacological activity and cost-effectiveness consideration, rhein and icariin were selected to self-assemble to construct NPs. The prepared NPs show good anti-inflammatory properties and alleviate ferroptosis in vitro and in vivo. Further research has found that the prepared rhein–icariin NPs (R–I NPs) mainly prolong the survival time of mice by maintaining the kidneys’ function in sepsis, which is consistent with the function of their formulation Compound rhubarb granule. The prepared carrier-free, safe, and traditional Chinese medicine (TCM)-derived NPs show great potential in drug delivery. This study also provides a safe and simple nanoplatform to treat sepsis, which may pave a way to design TCM-derived NPs for clinical translation.

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Mechanisms and efficacy of traditional Chinese herb monomers in diabetic kidney disease

Cited by 3 publications

References 119 publications

Mechanism of Huaiqihuang in treatment of diabetic kidney disease based on network pharmacology, molecular docking and in vitro experiment

Mechanism of Huaiqihuang in treatment of diabetic kidney disease based on network pharmacology, molecular docking and in vitro experiment

Chinese herbal medicine for the treatment of diabetic nephropathy: From clinical evidence to potential mechanisms

Carrier-Free Small Molecule-Assembled Nanoparticles for Treatment of Sepsis

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