Mechanisms and functions of long noncoding RNAs in intervertebral disc degeneration

Ran, Ruiqiong; Liao, Hai-Yang; Wang, Zhiqiang; Gong, Chang; Zhou, Kaisheng; Zhang, Haihong

doi:10.1016/j.prp.2022.153959

Cited by 5 publications

(3 citation statements)

References 139 publications

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“…EVs, exosomes, and ncRNAs are involved in the regulation of gene expression related to ferroptosis, which has emerged as a potential therapeutic strategy for IVDD ( 116 , 145 , 146 ). The upregulation of miR-10a-5p inhibited IL-6R expression, thereby partially reducing IL-6-induced ferroptosis in chondrocytes ( 45 ).…”

Section: Ferroptosis and Ivdd Treatmentmentioning

confidence: 99%

Ferroptosis: A potential target for the intervention of intervertebral disc degeneration

et al. 2022

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Ferroptosis, an iron-dependent form of programmed cell death marked by phospholipid peroxidation, is regulated by complex cellular metabolic pathways including lipid metabolism, iron balance, redox homeostasis, and mitochondrial activity. Initial research regarding the mechanism of ferroptosis mainly focused on the solute carrier family 7 member 11/glutathione/glutathione peroxidase 4 (GPX4) signal pathway. Recently, novel mechanisms of ferroptosis, independent of GPX4, have been discovered. Numerous pathologies associated with extensive lipid peroxidation, such as drug-resistant cancers, ischemic organ injuries, and neurodegenerative diseases, are driven by ferroptosis. Ferroptosis is a new therapeutic target for the intervention of IVDD. The role of ferroptosis in the modulation of intervertebral disc degeneration (IVDD) is a significant topic of interest. This is a novel research topic, and research on the mechanisms of IVDD and ferroptosis is ongoing. Herein, we aim to review and discuss the literature to explore the mechanisms of ferroptosis, the relationship between IVDD and ferroptosis, and the regulatory networks in the cells of the nucleus pulposus, annulus fibrosus, and cartilage endplate to provide references for future basic research and clinical translation for IVDD treatment.

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Section: Ferroptosis and Ivdd Treatmentmentioning

confidence: 99%

Ferroptosis: A potential target for the intervention of intervertebral disc degeneration

et al. 2022

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“…To date, changes in molecular expression are also considered to be a crucial contributor to the development of IDD, and several coding genes associated with IDD have been identified [ 3 ]. In addition to the coding genes, there has recently been increasing attention to the effect of non-coding RNAs in the pathogenesis of IDD [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Verification of Key MiRNAs Associated with Intervertebral Disc Degeneration

Liu

2023

CCHTS

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Background: Intervertebral disc degeneration (IDD) is a heterogeneous spinal disease, and its underlying molecular mechanism is unclear. Objectives: This study was aimed to identify, profile, and analyze microRNAs (miRNAs) that are related to IDD. Method: Microarray gene expression IDD data (GSE63492) were downloaded from Gene Expression Omnibus datasets. We employed weighted gene co-expression network analysis (WGCNA) to construct a miRNA co-expression network and the miRNAs related to IDD stage were detected. The number of differentially expressed miRNAs between normal and degenerated nucleus pulposus tissues was calculated. Twenty-three clinical specimens were used to validate the expression of miRNAs using qRT-PCR. Results: WGCNA identified 48 miRNAs that were significantly related to IDD stage, and 94 miRNAs were significantly different between normal and degenerated nucleus pulposus tissues. We selected 32 overlapping miRNAs and identified 347 corresponding target genes. The integrative analysis revealed the biological function and pathways of these targeted genes. Analysis of clinical specimens validated that hsa-miR-4534 was upregulated in IDD, whereas hsa-miR-1827 and hsa-miR-185-5p were downregulated in IDD.

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“…Additionally, many gene polymorphisms are linked to LDH, such as VDR [ 10 ], STOX1 [ 11 ], and MIR31HG [ 12 ]. Recently, research reveals that long noncoding RNAs (lncRNAs) play a critical role in biological processes, with abnormal expression linked to human diseases such as intervertebral disc degeneration, and osteoarthritis [ 13 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Association of LINC-PINT polymorphisms with lumbar disc herniation risk among Chinese Han population: a case control study

Bai

et al. 2023

J Orthop Surg Res

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Background Lumbar disc herniation (LDH) is a complex spinal disease, with multiple genetic polymorphisms being related to its risk. Nevertheless, the role of LINC-PINT polymorphisms in LDH risk has remained unknown. Therefore, this study aimed to investigate the association between LINC-PINT polymorphisms and LDH risk. Methods DNA was extracted from 504 LDH patients and 500 healthy controls. Three single nucleotide polymorphisms (SNPs) in LINC-PINT were selected and genotyped using Agena MassARRAY. We used logistic regression analysis to calculate odds ratios (ORs) and 95% confidence intervals (95% CIs) under multiple genetic models to evaluate the association between LINC-PINT polymorphisms and LDH risk. Haploview 4.2 and SNPStats software were used to evaluate the linkage strength of SNPs and the correlation between haplotypes and LDH risk. The impact of SNP-SNP interactions on LDH risk was analyzed using multi-factor dimensionality reduction (MDR). Results Results showed that rs157916 (G vs. A: OR = 1.23, FDR-p = 0.029) and rs7801029 (G vs. C: OR = 1.39, FDR-p = 0.006; GG vs. CC: OR = 2.34, FDR-p = 0.038; recessive: OR = 2.13, FDR-p = 0.045; additive: OR = 1.39, FDR-p = 0.030) were associated with an increased risk of LDH. Furthermore, LINC-PINT rs157916 and rs780129 were found to be significantly associated with LDH risk in males. The “GGG” haplotype was associated with increased LDH risk (OR = 1.41, FDR-p = 0.006). MDR analysis indicated that the interaction between rs7801029 and rs16873842 was associated with an increased risk of LDH (OR = 1.47, p = 0.004). Additionally, there were significant differences in C-reactive protein levels among different genotypes of rs157916 and rs780129 (p < 0.05). Conclusion This study suggests that LINC-PINT gene polymorphisms (rs157916 and rs7801029) are considered risk factors for LDH in the Chinese Han population and provide a scientific basis for early screening, prevention, and diagnosis of LDH.

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Mechanisms and functions of long noncoding RNAs in intervertebral disc degeneration

Cited by 5 publications

References 139 publications

Ferroptosis: A potential target for the intervention of intervertebral disc degeneration

Ferroptosis: A potential target for the intervention of intervertebral disc degeneration

Identification and Verification of Key MiRNAs Associated with Intervertebral Disc Degeneration

Association of LINC-PINT polymorphisms with lumbar disc herniation risk among Chinese Han population: a case control study

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