Mechanisms by which benzo[a]pyrene, an environmental carcinogen, suppresses B cell lymphopoiesis

Hardin, Janet A.; Hinoshita, Fumihiko; Sherr, David H.

doi:10.1016/0041-008x(92)90232-h

Cited by 71 publications

(35 citation statements)

References 39 publications

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“…For the fluorescence assay for apoptosis, quantitation of apoptotic cells was performed as previously described (26,30). Briefly, cells were washed in cold PBS, pelleted, and resuspended in 1.5 ml of hypotonic fluorochrome solution containing 5.0 g of propidium iodide (PI) (Sigma Chemical Co.) per ml, 1% sodium citrate, and 0.1% Triton X-100 (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of c-myc Expression Induces Apoptosis of WEHI 231 Murine B Cells

Arsura

Bellas

et al. 1996

Molecular and Cellular Biology

133

110

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Section: Methodsmentioning

confidence: 99%

Inhibition of c-myc Expression Induces Apoptosis of WEHI 231 Murine B Cells

Arsura

Bellas

et al. 1996

Molecular and Cellular Biology

133

110

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“…When we transfected pEGFP-53BP2 (full) plasmid into HeLa and COS-1 cells, we observed that a signi®cant number of cells exhibited features characteristic for apoptosis such as rounded cell morphology with shrinking cytoplasm, blebbing and fragmented nuclei and condensed chromatin (Bellamy et al, 1995;Hardin et al, 1992) (Figure 6). These cells were also stained positively with Annexin V-PE, another characteristic feature of apoptosis (Martin et al, 1995) (Figure 6d and e).…”

Section: Bp2 Induced Cell Death In the Transfected Cellsmentioning

confidence: 99%

“…On the other hand, nuclear factor kappa B (NF-kB) encompasses a family of inducible transcriptional factors and is a critical regulator of the cellular responses to various environmental challenges including infectious agents, oxidative stress and chemical agents (for reviews, see Baeuerle and Baichwal, 1997;Hardin et al, 1992;Okamoto et al, 1997). The NF-kB/Rel family is composed of p50, p65 (RelA), p52, RelB and c-Rel.…”

Section: Introductionmentioning

confidence: 99%

NF-κB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2

et al. 1999

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Nuclear factor kB (NF-kB) is a transcription factor that controls the expression of many cellular and viral genes. The p65 (RelA) subunit plays a critical role as a transcriptional activator and recent observations have highlighted its role in the control of apoptosis. Here we report that 53BP2, a protein previously identi®ed by interaction with wild type p53 and Bcl-2, also binds to p65 in a yeast two-hybrid system. This speci®c interaction was con®rmed by pull-down assay in vitro and by a mammalian two-hybrid assay in vivo. We observed that full-length 53BP2 fused to GFP had a punctate distribution in cytoplasm, predominantly in perinuclear region whereas the N-terminal 53BP2 localized in cytoplasm and C-terminal 53BP2 localized in the nucleus. Furthermore, we found that overexpression of GFP-53BP2 induced apoptosis in transiently transfected cells. Neither the N-terminal nor the Cterminal of 53BP2 fused to GFP induced cell death. Interestingly, co-transfection with a p65 expression plasmid signi®cantly inhibited 53BP2-induced cell death. The previous ®ndings that 53BP2 bound to p53 and Bcl-2 together with our present observations suggest that 53BP2 may play a central role in the regulation of apoptosis and cell growth.

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“…Furthermore, it represent one of the most significant classes of organic pollution due to their carcinogenic and mutagenic potential (Barra et al, 2001). Benzo-a-Pyrene (BaP) is a Polycyclic Aromatic Hydrocarbon (PAHs) which widespread in world environment due to their occurrence in petroleum, coal, soot, air pollutants and cutting oils (Hardin et al, 1992;Mudzinski, 1993). It releases to the environment through the incomplete combustion of gasoline, garbage or any organic material and finds its way to the aquatic environment through runoffs, oil spill, industrial effluent and atmospheric disposition (Lee et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

Effect of Black Mustard (Brassica nigra) on the Interaction between Immune and Biotransformation Systems of Nile Tilapia (Oreochromis niloticus) Exposed to Benzo-a-Pyrene

Abbas¹,

Awad²,

Abdel-Rahm³

2015

J. of Fisheries and Aquatic Science

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Aquatic pollutants are responsible form any alterations in fish health. Our study was designed to examine the efficacy of dietary supplement of black mustard seed, Brassica nigra (30% crude and 1% alcoholic extract) on enhancement the immunity and detoxification response of Nile tilapia, Oreochromis niloticus after exposed to a hydrocarbon pollutant; Benzo-a-Pyrene (BaP). Fish were divided into 3 groups before being fed for 28 days with crude black mustard seeds,1% alcoholic extract and un-supplemented commercial diet as the control. At the end of experiment, fish were exposed to 1 mg LG 1 of BaP for 24 h. Humoral immune parameters (lysozyme activity, antiprotease activity and total protein),ethoxyresorufin-o-deethylase (EROD) and hepatic cytochrome P450 1A1 content (CYP1A1) were investigated. Results recorded high significant difference (p<0.05) of antiprotease and total protein values in group fed with 1% extract followed by crude seeds as compared with control. The highest significant lysozyme activity (p<0.05) was recorded also in group fed with 1% extract as compared with other groups. On the other hand the extract group revealed a significant decrease in EROD (p<0.05) and CYP1A1 content compared to other groups. Which lowering the possible toxic effect of bio-transformed metabolic intermediate compounds.While the EROD activity and CYP1A1 content in group fed with the crude seeds showed increase over the control one. Therefore, the results suggest using of black mustard extract as a dietary supplement to increase the immune function and enhance the detoxification process of pollutants and hence, reduce the harmful effect of aquatic pollutants on Nile tilapia health.

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Mechanisms by which benzo[a]pyrene, an environmental carcinogen, suppresses B cell lymphopoiesis

Cited by 71 publications

References 39 publications

Inhibition of c-myc Expression Induces Apoptosis of WEHI 231 Murine B Cells

Inhibition of c-myc Expression Induces Apoptosis of WEHI 231 Murine B Cells

NF-κB subunit p65 binds to 53BP2 and inhibits cell death induced by 53BP2

Effect of Black Mustard (Brassica nigra) on the Interaction between Immune and Biotransformation Systems of Nile Tilapia (Oreochromis niloticus) Exposed to Benzo-a-Pyrene

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