Mechanisms for Binding between Methylene Blue and DNA

Vardevanyan, P. O.; Antonyan, Ara P.; Parsadanyan, Marine A.; Shahinyan, Mariam A.; Hambardzumyan, L. A.

doi:10.1007/s10812-013-9811-7

Cited by 81 publications

(50 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The catalytic activity of NH 2 -rGO/TNCC/SPE towards methylene blue (MB) was investigated by the cyclic voltammetric in three different conditions: (a) without ssPNA, (b) in the presence of ssPNA (5 μM), and (c) in the presence of PNA together with the MTB DNA target concentration introduced (1 × 10 -8 M), respectively. As shown in Figure 4(a), anodic peak potential at -0.26 V (curve (A), black line) has shown a reversible electron transfer reaction even without ssPNA on surface of the modified electrode, which can be attributed to the electrochemical reaction of the phenothiazine group [52]. Interestingly, CV peak displayed the negatively shifted peak, and increases of peak current after ssPNA were immobilized on the modified electrode (curve (B), blue line), which indicated that MB can be transported through ssPNA.…”

Section: Electrochemical Behavior Of Mb On the Modifiedmentioning

confidence: 95%

Reduced Graphene Oxide/TEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination ofMycobacterium tuberculosis

et al. 2020

View full text Add to dashboard Cite

A novel peptide nuclide acid (PNA) electrochemical biosensor based on reduced graphene oxide (NH2-rGO)/2,2,6,6-tetramethylpiperidin-1-yl)oxyl nanocrystalline cellulose (TEMPO-NCC) for the detection of Mycobacterium tuberculosis (M. Tuberculosis) is described. In this study, the nanohybrid films NH2-rGO/TEMPO-NCC were immobilized onto screen-printed carbon electrode (SPE) via a simple drop-coating method. The electrochemical characterization of the designed electrode was investigated using cyclic voltammetry (CV) and impedance spectroscopy (EIS). Meanwhile, the sensitivity and selectivity of the designed biosensor against M. tuberculosis were measured by the differential pulse voltammetry (DPV). The response of the PNA probe-modified (NH2-rGO)/TEMPO-NCC demonstrated that the fabricated biosensor was able to distinguish between complementary, noncomplementary, and one-base mismatch DNA sequences using methylene blue (MB) as the electrochemical indicator. The developed electrochemical biosensor exhibited a linear calibration curve in the concentration range of 1×10−8 M to 1×10−13 M with the limit of detection of 3.14×10−14 M. The developed electrochemical biosensor has also been tested with a polymerase chain reaction (PCR) product of M. tuberculosis DNA which has shown successful results in distinguishing between negative and positive samples of M. tuberculosis.

show abstract

Section: Electrochemical Behavior Of Mb On the Modifiedmentioning

confidence: 95%

Reduced Graphene Oxide/TEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination ofMycobacterium tuberculosis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…s. no.compoundmode of interactionbinding constant K (M −1 )change in λ max (nm)detection limit (ng µl −1 )references1 UA1 electrostatic7.2 × 10 3 241.8CW2 UA6 electrostatic2.4 × 10 3 425.8CW3 UA7 electrostatic0.2 × 10 3 224.0CW4AGelectrostatic10 4 1015[3]5EtBintercalation>10 5 441.0[29,30]6SYBR G-Igroove binding>10 6 270.06[4,31]7TOTO-Iintercalation10 9a —0.02[32]8YOYO-Iintercalation>10 10 —0.5[33–35]9methylene blueintercalation/groove binding>10 4 35.0[36,37]…”

Section: Resultsmentioning

confidence: 99%

Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

et al. 2016

View full text Add to dashboard Cite

DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1, UA6 and UA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 × 103, 2.4 × 103 and 0.2 × 103 M−1, for UA1, UA6 and UA7, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the λmax value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ng µl−1 for UA1, UA6 and UA7, respectively.

show abstract

“…Bell-like dependence of Δ on is inherent for EtBr [7] which is conditioned by several simultaneous binding modes of this ligand with DNA [6]. In the case of MB at 0.02 M ionic strength of solution two binding modes are displayed [25,28]. Consequently, at enhancement of EtBr and MB concentrations, redistribution of EtBr and MB bound molecules (displacement of MB bound molecules by EtBr molecules) occurs during melting.…”

Section: Resultsmentioning

confidence: 99%

Behavior of Ethidium Bromide-Hoechst 33258-DNA and Ethidium Bromide-Methylene Blue-DNA Triple Systems by means of UV Melting

Vardevanyan

Antonyan

Parsadanyan

et al. 2015

International Journal of Spectroscopy

View full text Add to dashboard Cite

The study of EtBr and H33258 interaction as well as EtBr and MB interaction with DNA has been carried out. It was revealed that, at joint interaction, the effect of two ligands on the change of melting thermodynamic parameters of EtBr-DNA-H33258 or EtBr-DNA-MB complexes is not an addition of separate interaction influences. It was shown that, at joint, binding of EtBr and MB with DNA competition occurs, while in the case of EtBr and H33258, the mutual strengthening of stabilizing effect of each of them on DNA double-stranded structure mainly takes place.

show abstract

Mechanisms for Binding between Methylene Blue and DNA

Cited by 81 publications

References 20 publications

Reduced Graphene Oxide/TEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination ofMycobacterium tuberculosis

Reduced Graphene Oxide/TEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination ofMycobacterium tuberculosis

Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

Behavior of Ethidium Bromide-Hoechst 33258-DNA and Ethidium Bromide-Methylene Blue-DNA Triple Systems by means of UV Melting

Contact Info

Product

Resources

About