Mechanisms involved in ATP-evoked Ca2+ oscillations in isolated human granulosa-luteal cells

Squires, Paul E.; Lee, P.S.N.; Yuen, Basil Ho; Leung, Peter C.K.; Buchan, Alison

doi:10.1016/s0143-4160(97)90030-0

Cited by 25 publications

(14 citation statements)

References 36 publications

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“…The [Ca 2ϩ ]i response to progesterone was not affected by nifedipine, but was reduced by Ni 2ϩ , which blocks mostly T-type Ca 2ϩ channels [Fox et al, 1987], but also Ca 2ϩ -release-activated channels (CRAC) [Dolmetsh and Lewis, 1994]. These channels are activated by depletion of intracellular stores in many cell types including granulosa cells [Squires et al, 1997], and are responsible for capacitative influx [Putney and Bird, 1993]. However, the response to progesterone was not altered by KCl [Machelon et al, 1996], although KCl induces cell depolarization and CRAC currents blocking [Dolmetsh and Lewis, 1994].…”

Section: Discussionmentioning

confidence: 99%

Phospholipase C-? and ovarian sex steroids in pig granulosa cells

Lieberherr

Grosse²,

Machelon³

1999

J. Cell. Biochem.

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We compared the membrane effects of estradiol, progesterone, and androstenedione in a single experimental model, the ovarian granulosa cells collected from immature Large White sows. We measured changes in cytosolic free calcium concentration ([Ca2+]i) in confluent Fura-2 loaded cells. We used pharmacological tools and polyclonal phospholipase C-beta (PLC-beta) antibodies. Each steroid (0.1 pM to 1 nM) transiently increased intracellular calcium concentration ([Ca2+]i) within 5 sec. They mobilized Ca2+ from the endoplasmic reticulum as shown by using two phospholipase C inhibitors, neomycin and U-73122. Ca2+ mobilization involved PLC-beta1 for progesterone, PLC-beta2 for estradiol and PLC-beta4 for androstenedione. A pertussis toxin-insensitive G protein was involved in the effects of progesterone on Ca2+ mobilization whereas estradiol and androstenedione effects were mediated via a pertussis toxin-sensitive G-protein. Ca2+ influx from the extracellular milieu was involved in the increase in [Ca2+]i induced by progesterone and estradiol, but not by androstenedione. Influx of Ca2+ was independent of Ca2+ mobilization from calcium stores, and it was suggested that L-type Ca2+ channels for estradiol and T-type Ca2+ channels for progesterone were involved. The three steroids had no effect on cAMP. Rapid effects of progesterone, estradiol, and androstenedione involved a direct action on cell membrane elements such as PLC-beta, G-proteins, and calcium channels, and these mechanisms were hormone-specific.

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Section: Discussionmentioning

confidence: 99%

Phospholipase C-? and ovarian sex steroids in pig granulosa cells

Lieberherr

Grosse²,

Machelon³

1999

J. Cell. Biochem.

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“…P2, but not P1, receptors were shown to be expressed on chicken granulosa cells [292]. Calcium oscillations were mediated by P2Y 2 and/or P2Y 4 receptors in human granulosa-luteal cells [228,385].…”

Section: Female Reproductive Organsmentioning

confidence: 99%

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

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There are multiple roles for purinergic signalling in both male and female reproductive organs. ATP, released as a cotransmitter with noradrenaline from sympathetic nerves, contracts smooth muscle via P2X1 receptors in vas deferens, seminal vesicles, prostate and uterus, as well as in blood vessels. Male infertility occurs in P2X1 receptor knockout mice. Both short-and long-term trophic purinergic signalling occurs in reproductive organs. Purinergic signalling is involved in hormone secretion, penile erection, sperm motility and capacitation, and mucous production. Changes in purinoceptor expression occur in pathophysiological conditions, including pre-eclampsia, cancer and pain.

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“…Ovarian sympathetic activity increases during the ovulatory process, but the neuronal content of NA and ATP decreases after ovulation. ATP evokes Ca 2+ oscillations in isolated human granulosa-luteal cells [504]. Granulosa cells secrete oestradiol and luteal cells secrete both oestradiol and progesterone.…”

Section: Ovarymentioning

confidence: 99%

“…P2, but not P1, receptors were also identified on chicken granulosa cells [361]. P2Y 2 and/or P2Y 4 receptors in human granulosa-luteal cells mediate calcium oscillations [294,504]. Granulosa cells in contact with the oocyte, respond to ATP via a mechanism that involves P2Y 2 receptor stimulation and the participation of ryanodine receptors [357].…”

Section: Ovarymentioning

confidence: 99%

Purinergic signalling in endocrine organs

Burnstock

2013

Purinergic Signalling

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There is widespread involvement of purinergic signalling in endocrine biology. Pituitary cells express P1, P2X and P2Y receptor subtypes to mediate hormone release. Adenosine 5′-triphosphate (ATP) regulates insulin release in the pancreas and is involved in the secretion of thyroid hormones. ATP plays a major role in the synthesis, storage and release of catecholamines from the adrenal gland. In the ovary purinoceptors mediate gonadotrophin-induced progesterone secretion, while in the testes, both Sertoli and Leydig cells express purinoceptors that mediate secretion of oestradiol and testosterone, respectively. ATP released as a cotransmitter with noradrenaline is involved in activities of the pineal gland and in the neuroendocrine control of the thymus. In the hypothalamus, ATP and adenosine stimulate or modulate the release of luteinising hormone-releasing hormone, as well as arginine-vasopressin and oxytocin. Functionally active P2X and P2Y receptors have been identified on human placental syncytiotrophoblast cells and on neuroendocrine cells in the lung, skin, prostate and intestine. Adipocytes have been recognised recently to have endocrine function involving purinoceptors.

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Mechanisms involved in ATP-evoked Ca2+ oscillations in isolated human granulosa-luteal cells

Cited by 25 publications

References 36 publications

Phospholipase C-? and ovarian sex steroids in pig granulosa cells

Phospholipase C-? and ovarian sex steroids in pig granulosa cells

Purinergic signalling in the reproductive system in health and disease

Purinergic signalling in endocrine organs

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