Phospholipase C-? and ovarian sex steroids in pig granulosa cells

Lieberherr, Michèle; Grosse, Brigitte; Machelon, Véronique

doi:10.1002/(sici)1097-4644(19990701)74:1<50::aid-jcb6>3.0.co;2-i

Cited by 27 publications

(14 citation statements)

References 45 publications

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“…In normal healthy porcine [35], human [36], and rat GCs [37] as well as SIGCs [25] [39][40][41]. These increases in [Ca 2ϩ ] i are sufficient to allow the transition from G 0 /G 1 to the S phase of the cell cycle [39].…”

Section: Discussionmentioning

confidence: 99%

Membrane-Initiated Events Account for Progesterone's Ability to Regulate Intracellular Free Calcium Levels and Inhibit Rat Granulosa Cell Mitosis1

Peluso

Fernández²,

Pappalardo³

et al. 2002

Biology of Reproduction

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It has been proposed that the antimitogenic action of progesterone (P(4)) is mediated through a membrane receptor that has GABA(A) receptor-like characteristics. To test this hypothesis, studies were designed to compare the antimitogenic effects of P(4) with its gamma amino butyric acid(A) (GABA(A)) receptor-activating metabolite, 5alpha-pregnane-3alpha-21-diol-20-one (5alpha3alpha). These studies revealed that P(4) was more effective than 5alpha3alpha in blocking mitogen-dependent mitosis of both small granulosa cells (GCs) and spontaneously immortalized granulosa cells (SIGCs). Ligand binding studies illustrated that P(4) bound to SIGCs with an apparent dissociation constant (K(d)) of 0.32 +/- 0.09 microM, whereas 5alpha3alpha bound with an apparent K(d) of 40 +/- 19 microM. Further, the GABA(A) antagonist, bicuculline, did not attenuate P(4)'s antimitotic action in SIGCs. Finally, reverse transcriptase-polymerase chain reaction (RT-PCR) studies demonstrated that none of the 6 known alpha chains of the GABA(A) receptors to which bicuculline binds were detected in SIGCs. Taken together, these studies suggest that P(4) does not mediate its action via a GABA(A)-like receptor. Additional studies revealed that P(4) regulated intracellular free calcium levels ([Ca(2+)](i)) as part of its antimitotic action. Specifically, P(4) maintained a basal [Ca(2+)](i) level that was slightly lower than normal. Increasing extracellular calcium not only increased basal [Ca(2+)](i) but also attenuated P(4)'s antimitogenic effect. P(4)'s actions appeared to be initiated at the membrane, since horseradish peroxidase conjugated-P(4) (HP-P(4)), which is cell impermeable, was as effective in blocking mitosis as P(4). Progesterone receptor (PR) mRNA was not detected in SIGCs by RT-PCR analysis, which is consistent with the findings in GCs. However, a 60-kDa protein was detected within crude membrane fractions of both GCs and SIGCs using an antibody directed against the ligand binding domain of the PR (C-262). This antibody was also used in immunocytochemical studies to detect a protein that was associated with the plasma membrane of SIGCs. It is proposed that this 60-kDa protein mediates P(4)'s membrane-initiated actions.

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Section: Discussionmentioning

confidence: 99%

Membrane-Initiated Events Account for Progesterone's Ability to Regulate Intracellular Free Calcium Levels and Inhibit Rat Granulosa Cell Mitosis1

Peluso

Fernández²,

Pappalardo³

et al. 2002

Biology of Reproduction

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“…Our results suggest that estradiol, p,p 0 -DDE, o,p-DDE and methoxychlor act through a pertussis toxin-sensitive G-protein, since pretreatment with the toxin inhibited the [Ca 2þ ] i response to stimulation by these agents. Estradiol and androstenedione act via a pertussis toxin-sensitive G-protein in porcine granulosa cells (Lieberherr et al 1999). On the other hand, the [Ca 2þ ] i response to progesterone and Kepone was not inhibited, suggesting that these two compounds acted through a pertussis toxin-insensitive G-protein.…”

Section: Nongenomic Effects Of Pesticides On Granulosa-lutein Cells 303mentioning

confidence: 90%

Rapid effects of pesticides on human granulosa-lutein cells

Y¹,

Foster²,

Younglai³

2006

Reproduction

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“…In addition, a rapid progesteroneinduced increase in the formation of inositol 1,4,5-trisphosphate (IP 3 ) and 1,2-diacylglycerol (DAG) was observed in these cells (191,251). Similarly, progesterone triggers an immediate and transient peak in [Ca 2ϩ ] i and an induction of IP 3 in porcine granulosa cells (257,272). The mentioned effects in osteoblasts and granulosa cells have been shown to be insensitive to RU 486 and were also triggered by progesterone bound to BSA, although at 100-fold higher concentrations.…”

Section: B Progesteronementioning

confidence: 93%

“…Furthermore, androstenedione, the main intrafollicular androgen, rapidly increased [Ca 2ϩ ] i in human granulosa luteinizing cells (273) and porcine ovarian granulosa cells (257). This increase was dose dependent, with maximum activity at 1 nM androstenedione in pig and 10 nM in human cells.…”

Section: Androgensmentioning

confidence: 93%

Nongenomic Steroid Action: Controversies, Questions, and Answers

Lösel

Falkenstein

Feuring

et al. 2003

Physiological Reviews

504

321

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Lösel, Ralf M., Elisabeth Falkenstein, Martin Feuring, Armin Schultz, Hanns-Christian Tillmann, Karin Rossol-Haseroth, and Martin Wehling. Nongenomic Steroid Action: Controversies, Questions, and Answers. Physiol Rev 83: 965–1016, 2003; 10.1152/physrev.00003.2003.—Steroids may exert their action in living cells by several ways: 1) the well-known genomic pathway, involving hormone binding to cytosolic (classic) receptors and subsequent modulation of gene expression followed by protein synthesis. 2) Alternatively, pathways are operating that do not act on the genome, therefore indicating nongenomic action. Although it is comparatively easy to confirm the nongenomic nature of a particular phenomenon observed, e.g., by using inhibitors of transcription or translation, considerable controversy exists about the identity of receptors that mediate these responses. Many different approaches have been employed to answer this question, including pharmacology, knock-out animals, and numerous biochemical studies. Evidence is presented for and against both the participation of classic receptors, or proteins closely related to them, as well as for the involvement of yet poorly understood, novel membrane steroid receptors. In addition, clinical implications for a wide array of nongenomic steroid actions are outlined.

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Phospholipase C-? and ovarian sex steroids in pig granulosa cells

Cited by 27 publications

References 45 publications

Membrane-Initiated Events Account for Progesterone's Ability to Regulate Intracellular Free Calcium Levels and Inhibit Rat Granulosa Cell Mitosis1

Membrane-Initiated Events Account for Progesterone's Ability to Regulate Intracellular Free Calcium Levels and Inhibit Rat Granulosa Cell Mitosis1

Rapid effects of pesticides on human granulosa-lutein cells

Nongenomic Steroid Action: Controversies, Questions, and Answers

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