Mechanisms Involved in the Enhancement of Allergic Airways Neutrophil Influx by Permanent C-Fiber Degeneration in Rats

Abstract

This study was undertaken to clarify the mechanisms by which C-fiber degeneration at neonatal stages exacerbates the inflammatory responses of rat airways. Rats were treated with capsaicin at neonatal stages and immunized with ovalbumin (OVA) at adult ages. Challenge of capsaicin-pretreated rats with OVA promoted a higher influx of neutrophils in bronchoalveolar lavage (BAL) fluid compared with the vehicle group. No significant differences were found for the other cell types. The increased adhesion of N-formyl-methionyl-leucyl-phenylalanine (fMLP; 0.1 M)-and phorbol myristate acetate (PMA; 1 M)-treated neutrophils to fibronectin-coated wells did not differ among vehicle-and capsaicin-pretreated rats. Additionally, fMLP (10 M), platelet-activating factor (0

Metalloelastase in lungs and alveolar macrophages is modulated by extracellular substance P in mice

“…Many studies have demonstrated that CS vigorously stimulates PCFs (9, 29 -31), leading to a significant increase of pulmonary SP in guinea pigs (26), mice (61), and COPD patients (53). NCAP treatment has been reported to reduce pulmonary SP protein by 75-90% and efficiently cause PCF degeneration in rats (12,23,55). Consistent with these results, we found that SP and PPT-A mRNA in the lungs were diminished by 75 and 68%, respectively.…”

Pre-exposure to Staphylococcal enterotoxin A exacerbates the pulmonary allergic eosinophil recruitment in rats

“…We actively sensitized the animals by subcutaneous injections of 200 µg OVA dissolved in 0.15 ml of aluminum hydroxide (200 mg/ml in 0.9% NaCl), according to our previous experience [20]. Non-sensitized animals were injected with aluminum hydroxide alone.…”

Low level laser therapy partially restores trachea muscle relaxation response in rats with tumor necrosis factor α‐mediated smooth airway muscle dysfunction

“…In addition, bronchial epithelial cells are upregulated in their synthesis and release of granulocyte macrophage-colony-stimulating factor (GM-CSF). Collectively, these agents have been found to exert a diversity of actions including induction of specific IgE production by mononuclear cells [9][10][11], antigen presentation by macrophages, activation of airway structural cells [12][13][14], altered expression of adhesion molecules [15][16][17], induction of secretion of various growth factors [16], and recruitment of inflammatory cells from the circulation [18][19][20].…”