Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria

Durall, Claudia; Lindblad, Peter

doi:10.1016/j.algal.2015.07.002

Cited by 54 publications

(26 citation statements)

References 85 publications

(160 reference statements)

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“…Two identified orders of the phylum Chloroflexi, Chloroflexales and Herpetosiphonales, as well as the class Alphaproteobacteria of the phylum Proteobacteria, contain members capable of carbon assimilation (Campbell et al, 2014;Imam et al, 2015). The photoautotrophic phylum Cyanobacteria, which was also consistently present throughout the three treatment groups at a low mean abundance (Figures 3,4), is capable of carbon acquisition and therefore potentially plays a role in energy metabolism (Pointing et al, 2009;Durall and Lindblad, 2015).…”

Section: Microbial Community Potential Functionality Analysismentioning

confidence: 99%

Antarctic microbial communities are functionally redundant, adapted and resistant to short term temperature perturbations

Scally

Makhalanyane

Frossard

et al. 2016

Soil Biology and Biochemistry

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Climate change has the potential to induce dramatic shifts in the biodiversity and functionality of soil microorganisms in polar hyperarid ecosystems. In these depauperate soil ecosystems, microbial communities are vital as they represent the dominant input sources of essential nutrients. However, the effects of changing climate on extreme edaphic environments, such as the McMurdo Dry Valleys of Antarctica, remain poorly understood. To better understand these effects, we constructed soil microcosms and simulated temperature shifts over a 40-day period. Soil physicochemical analysis revealed low levels of key nutrients, with mean organic carbon and nitrogen contents of <0.1% and 11.55 ppm, respectively. We also applied 16S rRNA gene amplicon sequencing to determine taxonomic composition and enzyme assays to measure in situ activity. Our data showed a prevalence of ubiquitous soil taxa (Actinobacteria, Chloroflexi and Deinococcus-Thermus), with a smaller proportion of autotrophic phyla (i.e. Cyanobacteria). None of the major phyla showed relative abundance changes in response to temperature. We found very low extracellular enzyme activity levels across all samples and observed no significant differences among temperature treatments. Functional predictions (using PICRUSt) revealed the putative presence of key genes implicated in the cycling of carbon (ppc, rbcl) and nitrogen (nifH, nirK), in stress response and in DNA repair throughout all treatments. Overall, our results suggest that short-term temperature fluctuations do not alter microbial biodiversity and functionality in Antarctic soils. This study provides the first evidence that microbial communities within this edaphic extreme environment may be functionally redundant, adapted and resistant to short term climatic perturbations.

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Section: Microbial Community Potential Functionality Analysismentioning

confidence: 99%

Antarctic microbial communities are functionally redundant, adapted and resistant to short term temperature perturbations

Scally

Makhalanyane

Frossard

et al. 2016

Soil Biology and Biochemistry

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“…The oxygenation reaction of RuBisCO produces toxic 2-phosphoglycolate, which is converted into 3-phosphoglycerate (3PGA) by the photorespiratory pathway at the expense of releasing previously fixed carbon. Significant efforts have been made with the aim to improve the carboxylation rate or the RuBisCO discrimination between CO 2 and O 2 but with very limited success (Durall and Lindblad, 2015;Greene et al, 2007;Whitney et al, 2011). Indeed, these biotechnological attempts to improve RuBisCO suggest that the natural RuBisCOs may be nearly perfectly optimized despite its natural flaws (Tcherkez et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Liang

Lindblad

2016

Metabolic Engineering

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“…They appeared more than 2.5 billion years ago and were responsible for raising the oxygen levels in the atmosphere from being anoxic to about 20% [1][2][3]. Cyanobacteria fix inorganic carbon mainly through the Calvin-Benson-Bassham cycle using the inefficient ribulose 1,5-bisphosphate carboxylase/ oxygenase (RuBisCO), a similar system as C3 plants [4,5].…”

Section: Introductionmentioning

confidence: 99%

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Durall

Lindberg

et al. 2020

Biotechnol Biofuels

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Background: Cyanobacteria can be metabolically engineered to convert CO 2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. Results: The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed significantly increased ethylene production (10.5 ± 3.1 µg mL −1 OD −1 day −1) compared to the control strain (6.4 ± 1.4 µg mL −1 OD −1 day −1). Interestingly, extra copies of the native pepc or the heterologous expression of PEPc from the cyanobacterium Synechococcus PCC 7002 (Synechococcus) in the CD-P, increased ethylene production (19.2 ± 1.3 and 18.3 ± 3.3 µg mL −1 OD −1 day −1 , respectively) when the cells were treated with the acetyl-CoA carboxylase inhibitor, cycloxydim. A heterologous expression of phosphoenolpyruvate synthase (PPSA) from Synechococcus in the CD-P also increased ethylene production (16.77 ± 4.48 µg mL −1 OD −1 day −1) showing differences in the regulation of the native and the PPSA from Synechococcus in Synechocystis. Conclusions: This work demonstrates that genetic rewiring of cyanobacterial central carbon metabolism can enhance carbon supply to the TCA cycle and thereby further increase ethylene production.

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Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria

Cited by 54 publications

References 85 publications

Antarctic microbial communities are functionally redundant, adapted and resistant to short term temperature perturbations

Antarctic microbial communities are functionally redundant, adapted and resistant to short term temperature perturbations

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

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