Mechanisms of damage to the lung surfactant system

Balis, John U.; Shelley, Sue A.; McCue, Miles J.; Rappaport, Edward S.

doi:10.1016/0014-4800(71)90069-4

Cited by 34 publications

(3 citation statements)

References 27 publications

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“…Tubular myelin has been observed in alveolar washings (1,2) as well as in situ (3-7, 11, 12). Gil and Reiss (13) have reported the separation of tubular myelin from other membrane forms in lung homogenates.…”

Section: Discussionmentioning

confidence: 99%

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Linear arrays of intramembranous particles in pulmonary tubular myelin.

Emil

Lagunoff

1978

Proc. Natl. Acad. Sci. U.S.A.

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Freeze-fracture preparations of tubular myelin in edematous rat lungs reveal the presence of linear arrays of intramembranous particles. The lines of particles are approximately 50 nm apart and appear to correspond to the intersections of sheets of bilayer membranes. Particles are not seen in lamellar bodies but become evident first in membranes transitional between lamellar bodies and tubular myelin. It is proposed that the particles represent a hydrophobic protein that plays a significant role in the organization of tubular myelin. Tubular myelin isa highly ordered array of phospholipids and proteins found in pulmonary alveoli. Interest in tubular myelin arises from its presence as a major alveolar form of surfaceactive material (1, 2). Details of its unique structure have been described by a number of investigators (3-7).Tubular myelin occurs in normal adult lung but is particularly prominent in unaerated fetal lung, in which its derivation from lamellar bodies has been reported by Williams (8). Increased amounts of tubular myelin have been described in association with lung injury (6, 9), and we have observed considerable tubular myelin in the edema fluid induced by epinephrine in adult rats. We report here the results of our studies on the ultrastructure features of such tubular myelin. The presence of orderly, linear arrays of intramembranous particles in freeze-fracture preparations adds to the morphologic basis for the detailed analysis of the molecular structure and function of tubular myelin.MATERIALS AND METHODS Pulmonary edema was induced in 2-to 3-mo-old male Sprague-Dawley rats (Tyler Laboratories, Bellevue, WA) by the intravenous administration of epinephrine. Rats were anesthetized lightly with ether, their abdomens were opened, and epinephrine was injected into the inferior vena cava. Doses ranged from 0.1 to 0.2 ml of saline containing 0.1 mg of epinephrine per ml. Pulmonary edema developed rapidly; the thorax was opened between 3 and 4 min after epinephrine administration. The lungs were removed and fixed by immersion in 2% (vol/vol) glutaraldehyde/1% (vol/vol) formaldehyde (freshly prepared from paraformaldehyde)/0

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Section: Discussionmentioning

confidence: 99%

“…Tubular myelin isa highly ordered array of phospholipids and proteins found in pulmonary alveoli. Interest in tubular myelin arises from its presence as a major alveolar form of surfaceactive material (1,2). Details of its unique structure have been described by a number of investigators (3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

Linear arrays of intramembranous particles in pulmonary tubular myelin.

Emil

Lagunoff

1978

Proc. Natl. Acad. Sci. U.S.A.

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“…In ARDS, substantial leakage of plasma proteins, such as albumin, C-reactive protein, hemoglobin and fibrinogen, and of inflammatory mediators occurs in the alveolar spaces [30,31]. The lack of efficacy of RLS therapy in ARDS results from the fact that plasma proteins and mediators exasperate NLS function deterioration [32,33]. Numerous in vitro studies have demonstrated the surfactant inhibition properties of plasma proteins, in particular albumin [34,35].…”

Section: Introductionmentioning

confidence: 99%

Impact of perfluorooctylethane on the formation of a semi-crystalline liquid-condensed phase in a phospholipid monolayer and of perfluorooctyl bromide on the adsorption of albumin on such a monolayer

Gerber¹,

Vandamme²,

Krafft³

2008

Comptes Rendus. Chimie

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A comparison of freeze‐substitution with other methods for preservation of the pulmonary alveolar lining layer

Kuhn

1972

Am. J. Anat.

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Rapid freezing of expanded lungs and freeze-substitution in acetone containing OsOa partially preserved the pulmonary alveolar lining layer for electron microscopy. The lining layer consisted of granular or flocculent material with a surface film at its interface with the air. It filled the spaces between protruding capillaries and other depressions in the alveolar wall. It is unlikely that this lining material is an artifact resulting from transfer of fluid during freezing because similar results were obtained after fixation by vascular perfusion and by immersion of the intact, air-filled lung in aqueous fixatives containing glutaraldehyde.

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Mechanisms of damage to the lung surfactant system

Cited by 34 publications

References 27 publications

Linear arrays of intramembranous particles in pulmonary tubular myelin.

Linear arrays of intramembranous particles in pulmonary tubular myelin.

Impact of perfluorooctylethane on the formation of a semi-crystalline liquid-condensed phase in a phospholipid monolayer and of perfluorooctyl bromide on the adsorption of albumin on such a monolayer

A comparison of freeze‐substitution with other methods for preservation of the pulmonary alveolar lining layer

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