2000
DOI: 10.1046/j.1432-1033.2000.01769.x
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Mechanisms of differential transferrin receptor expression in normal hematopoiesis

Abstract: We have investigated the expression of transferrin receptor (TfR) iron regulatory protein-1 (IRP-1) and iron regulatory protein-2 (IRP-2) in liquid suspension culture of purified hematopoietic progenitor cells (HPCs) induced by a growth factor stimulus to proliferation and unilineage differentiation/maturation through the erythroid, granulocytic, monocytic and megakaryocytic lineages.In initial HPC differentiation, TfR expression is induced in both erythroid and granulopoietic cultures. In late HPC differentia… Show more

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Cited by 42 publications
(38 citation statements)
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“…This hyper-up-regulation of CD71/ TfR in early precursors (R1 to R2) is completely Epo-dependent, 16 and mediated via TfR gene transcriptional and posttranscriptional mechanisms. 17 In agreement with Epo-dependent CD71/ TfR up-regulation, overexpression of constitutive active Stat5 in primary erythroblasts, one of the major signaling molecules induced by Epo, led to Epo-independent CD71/TfR upregulation (data not shown).…”
Section: Lnk Inhibits Epo-dependent Erythroblast Differentiationmentioning
confidence: 60%
“…This hyper-up-regulation of CD71/ TfR in early precursors (R1 to R2) is completely Epo-dependent, 16 and mediated via TfR gene transcriptional and posttranscriptional mechanisms. 17 In agreement with Epo-dependent CD71/ TfR up-regulation, overexpression of constitutive active Stat5 in primary erythroblasts, one of the major signaling molecules induced by Epo, led to Epo-independent CD71/TfR upregulation (data not shown).…”
Section: Lnk Inhibits Epo-dependent Erythroblast Differentiationmentioning
confidence: 60%
“…Among the five remaining CGs with medium expression on ABI plate, three were discarded due to minor reasons: HPRT and PGK (X-chromosomal location) and TFRC (variable expression in hematopoietic cells). 33,34 Among the six additional primer and probe sets, covering four CGs, in-house B2M RQ-PCR set appeared to be suitable since no statistically significant difference between PB and BM was observed. Comparison between normal and leukemic samples was not possible since only four normal samples were tested (see Material and methods).…”
Section: Control Genes For Diagnosis and Mrd Detection By Rq-pcr E Bementioning
confidence: 99%
“…This regulation explains the increase observed in the expression of TfR1 in erythroid cells in conditions of iron deficiency. 34 However, the respective roles of IRP1 and IRP2 in erythroid cells is not clear since IRP1 knock-out mice have no erythroid phenotype 35 whereas IRP2 knock-out mice develop microcytic hypochromic anemia probably resulting from decreased TfR1 expression in bone marrow cells. 36,37 This standard regulation of iron metabolism seems to have an alternative model during terminal erythroid differentiation.…”
Section: The Iron Sensing Pathwaymentioning
confidence: 99%