Mechanisms of histone H3 lysine 27 trimethylation remodeling during early mammalian development

Bogliotti, Y. S.; Ross, Pablo J.

doi:10.4161/epi.21615

Cited by 52 publications

(36 citation statements)

References 68 publications

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“…Next‐generation sequencing technologies in concert with motif analysis provide a unique avenue to improve the atlas of potential CP‐regulated genes aiding in the determination of the combinatorial code by which oocytes regulate translation. It is conceivable that many unknown targets of CP are associated with functions other than cell cycle regulation, as early embryos require epigenetic reprogramming driven by stored transcripts to foster activation of transcription (embryonic genome activation) . In addition, some of the activated factors in MII oocytes are known contributors in embryos, suggesting the oocyte not only uses stored transcripts to drive oocyte maturation but also in preparation for fertilization and early embryo development.…”

Section: Resultsmentioning

confidence: 99%

Cytoplasmic polyadenylation in mammalian oocyte maturation

Reyes

Ross

2015

WIREs RNA

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Oocyte developmental competence is the ability of the mature oocyte to be fertilized and subsequently drive early embryo development. Developmental competence is acquired by completion of oocyte maturation, a process that includes nuclear (meiotic) and cytoplasmic (molecular) changes. Given that maturing oocytes are transcriptionally quiescent (as are early embryos), they depend on post-transcriptional regulation of stored transcripts for protein synthesis, which is largely mediated by translational repression and deadenylation of transcripts within the cytoplasm, followed by recruitment of specific transcripts in a spatiotemporal manner for translation during oocyte maturation and early development. Motifs within the 3' untranslated region (UTR) of messenger RNA (mRNA) are thought to mediate repression and downstream activation by their association with binding partners that form dynamic protein complexes that elicit differing effects on translation depending on cell stage and interacting proteins. The cytoplasmic polyadenylation (CP) element, Pumilio binding element, and hexanucleotide polyadenylation signal are among the best understood motifs involved in CP, and translational regulation of stored transcripts as their binding partners have been relatively well-characterized. Knowledge of CP in mammalian oocytes is discussed as well as novel approaches that can be used to enhance our understanding of the functional and contributing features to transcript CP and translational regulation during mammalian oocyte maturation. WIREs RNA 2016, 7:71-89. doi: 10.1002/wrna.1316 For further resources related to this article, please visit the WIREs website.

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Section: Resultsmentioning

confidence: 99%

Cytoplasmic polyadenylation in mammalian oocyte maturation

Reyes

Ross

2015

WIREs RNA

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“…It is catalyzed by enhancer of zeste homolog 2 (EZH2), and can be removed by H3K27me3-specific demethylase of Jumonji domain containing protein 3 (JMJD3) and ubiquitously transcribed tetratricopeptide repeat X (UTX) (Bogliotti & Ross 2012). And EZH2 and JMJD3/UTX can be specifically suppressed by two small molecule inhibitors of GSK126 and GSK-J4 respectively (Kruidenier et al 2012, McCabe et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Histone H3 lysine 27 trimethylation acts as an epigenetic barrier in porcine nuclear reprogramming

et al. 2016

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Aberrant epigenetic reprogramming is the main obstacle to the development of somatic cell nuclear transfer (SCNT) embryos and the generation of induced pluripotent stem (iPS) cells, which results in the low reprogramming efficiencies of SCNT and iPS. Histone H3 lysine 27 trimethylation (H3K27me3), as a repressive epigenetic mark, plays important roles in mammalian development and iPS induction. However, the reprogramming of H3K27me3 in pig remains elusive. In this study, we showed that H3K27me3 levels in porcine early cloned embryos were higher than that in IVF embryos. Then GSK126 and GSK-J4, two small molecule inhibitors of H3K27me3 methylase (EZH2) and demethylases (UTX/JMJD3), were used to regulate the H3K27me3 level. The results showed that H3K27me3 level was reduced in cloned embryos after treatment of PEF with 0.75 mM GSK126 for 48 h, incubation of one-cell reconstructed oocytes with 0.1 mM GSK126 and injection of antibody for EZH2 into oocyte. Meanwhile, the development of the cloned embryos was significantly improved after these treatments. On the contrary, GSK-J4 treatment increased the H3K27me3 level in cloned embryos and decreased the cloned embryonic development. Furthermore, iPS efficiency was both increased after reducing the H3K27me3 level in donor cells and in early reprogramming phase. In summary, our results suggest that H3K27me3 acts as an epigenetic barrier in SCNT and iPS reprogramming, and reduction of H3K27me3 level in donor cells and in early reprogramming phase can enhance both porcine SCNT and iPS efficiency.

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“…Incomplete ZGA is largely caused by certain key epigenetic barriers [8]. Histone modifications play crucial roles in regulating the expression of zygotic genes [9, 10] and are highly dynamic, with extensive changes during mammalian preimplantation embryo development [11, 12]. The down-regulation of H3K9me3 and H3K4me3 could improve transcriptional reprogramming of mouse SCNT preimplantation embryos and the production of cloned descendants [13].…”

Section: Introductionmentioning

confidence: 99%

Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos

Zhang¹,

Zhai²,

Ma³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Aberrantly high levels of H3K4me3, caused by incomplete epigenetic reprogramming, likely cause a low efficiency of somatic cell nuclear transfer (SCNT). Smal molecule inhibitors aimed at epigenetic modification can be used to improve porcine SCNT embryo development. In this study, we examined the effects of MM-102, an H3K4 histone methyltransferase inhibitor, on porcine SCNT preimplantation embryos to investigate the mechanism by which H3K4 methylation regulated global epigenetic reprograming during SCNT. Methods: MM-102 was added to the SCNT embryos culture system and the global levels of various epigenetic modifications were measured by immunofluorescence (IF) staining and were quantified by Image J software. Relative genes expression levels were detected by quantitative real-time PCR. Results: MM-102 (75 μM) treatment reduced global H3K4, H3K9 methylation and 5mC levels especially at the zygotic gene activation (ZGA) and blastocyst stages. MM-102 treatment mainly down-regulated a series of DNA and histone methyltransferases, and up-regulated a number of hitone acetyltransferases and transcriptional activators. Furthermore, MM-102 treatment positively regulated the mRNA expression of genes related to pluripotency (OCT4, NANOG, CDX2) and apoptosis (BCL2). Conclusion: Down-regulation of H3K4me3 with MM-102 rescued aberrant gene expression patterns of a series of epigenetic chromatin modification enzymes, pluripotent and apoptotic genes at the ZGA and blastocyst stages, thereby greatly improving porcine SCNT efficiency and blastocyst quality, making them more similar to in vivo embryos (IVV).

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Mechanisms of histone H3 lysine 27 trimethylation remodeling during early mammalian development

Cited by 52 publications

References 68 publications

Cytoplasmic polyadenylation in mammalian oocyte maturation

Cytoplasmic polyadenylation in mammalian oocyte maturation

Histone H3 lysine 27 trimethylation acts as an epigenetic barrier in porcine nuclear reprogramming

Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos

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