Mechanisms of intracellular ice formation

Muldrew, Ken; McGann, Locksley E.

doi:10.1016/s0006-3495(90)82568-6

Cited by 209 publications

(63 citation statements)

References 17 publications

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“…Freeze–thaw injuries to yeast cells may depend on many factors, including the genetic background of the yeast strains, the physiological condition of the yeast cells and the freezing conditions, such as the length of the freezing period and the speed of freezing. During frozen‐dough baking, yeast cells are frozen in a process that subjects them to low temperature, ice‐ crystal formation in the cells and dehydration [10,11]. Freezing can cause not only deleterious damage to the cell wall and membrane but also denaturation of functional proteins and DNA, thus decreasing cell viability.…”

Section: Baking‐associated Environmental Stressesmentioning

confidence: 99%

Stress‐tolerance of baker's‐yeast (Saccharomyces cerevisiae) cells: stress‐protective molecules and genes involved in stress tolerance

Shima

Takagi

2009

Biotech and App Biochem

100

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During the fermentation of dough and the production of baker's yeast (Saccharomyces cerevisiae), cells are exposed to numerous environmental stresses (baking-associated stresses) such as freeze-thaw, high sugar concentrations, air-drying and oxidative stresses. Cellular macromolecules, including proteins, nucleic acids and membranes, are seriously damaged under stress conditions, leading to the inhibition of cell growth, cell viability and fermentation. To avoid lethal damage, yeast cells need to acquire a variety of stress-tolerant mechanisms, for example the induction of stress proteins, the accumulation of stress protectants, changes in membrane composition and repression of translation, and by regulating the corresponding gene expression via stress-triggered signal-transduction pathways. Trehalose and proline are considered to be critical stress protectants, as is glycerol. It is known that these molecules are effective for providing protection against various types of environmental stresses. Modifications of the metabolic pathways of trehalose and proline by self-cloning methods have significantly increased tolerance to baking-associated stresses. To clarify which genes are required for stress tolerance, both a comprehensive phenomics analysis and a functional genomics analysis were carried out under stress conditions that simulated those occurring during the commercial baking process. These analyses indicated that many genes are involved in stress tolerance in yeast. In particular, it was suggested that vacuolar H+-ATPase plays important roles in yeast cells under stress conditions.

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Section: Baking‐associated Environmental Stressesmentioning

confidence: 99%

Stress‐tolerance of baker's‐yeast (Saccharomyces cerevisiae) cells: stress‐protective molecules and genes involved in stress tolerance

Shima

Takagi

2009

Biotech and App Biochem

100

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“…If the ice nuclei are small enough to be innocuous during cooling, they may still pose a danger to cell survival as they can become sites of recrystallization of large ice crystals later during the thawing process. Intracellular ice formation is typically lethal to cells [22–24]. An optimized cooling rate, as defined by Mazur, is expected to minimize the cell injury caused by both of these phenomena [5, 6].…”

Section: Cell Preservation Fundamentalsmentioning

confidence: 99%

Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

Wang

Elliott

2017

Curr Stem Cell Rep

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Purpose of the review Over the past several decades, cryopreservation has been widely used to preserve cells during long term storage, but advances in stem cell therapies, regenerative medicine, and miniaturized cell-based diagnostics and sensors are providing new targets of opportunity for advancing preservation methodologies. The advent of microfluidics-based devices is an interesting case in which the technology has been used to improve preservation processing, but as the devices have evolved to also include cells, tissues, and simulated organs as part of the architecture, the biochip itself is a desirable target for preservation. In this review, we will focus on the synergistic co-development of preservation methods and biochip technologies, while identifying where the challenges and opportunities lie in developing methods to place on-chip biologics on the shelf, ready for use. Recent findings Emerging studies are demonstrating that the cost of some biochips have been reduced to the extent that they will have high utility in point-of-care settings, especially in low resource environments where diagnostic capabilities are limited. Ice-free low temperature vitrification and anhydrous vitrification technologies will likely emerge as the preferred strategy for long-term preservation of bio-chips. Summary The development of preservation methodologies for partially or fully assembled biochips would enable the widespread distribution of these technologies and enhance their application.

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“…This has brought numerous researches on the freeze-thaw process and cell injury, either experimentally or numerically. [4][5][6][7][8][9][10][11] Two urgent needs for surgical applications are to explore mechanisms of cell injury that can maximize cell death within the tumor and minimize the damage to boundary cells which might be dead or live; or develop mature tools to control the cooling-thawing procedures that weaken the dependence of surgeons' experiences. Also deep investigations of cell destruction near the ice periphery can provide more information to evaluate the efficiency of cryotherapy and design feasible clinical tools.…”

Section: Introductionmentioning

confidence: 99%

Cell death along single microfluidic channel after freeze-thaw treatments

Wang

2010

Biomicrofluidics

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Cryotherapy is a prospective green method for malignant tumor treatment. At low temperature, the cell viability relates with the cooling rate, temperature threshold, freezing interface, as well as ice formation. In clinical applications, the growth of ice ball must reach a suitable size as cells could not be all killed at the ice periphery. The cell death ratio at the ice periphery is important for the control of the freezing destruction. The mechanisms of cryoinjury around the ice periphery need thorough understanding. In this paper, a primary freeze-thaw control was carried out in a cell culture microchip. A series of directional freezing processes and cell responses was tested and discussed. The temperature in the microchip was manipulated by a thermoelectric cooler. The necrotic and apoptotic cells under different cryotreatment ͑duration of the freezing process, freeze-thaw cycle, postculture, etc.͒ were stained and distinguished by propidium iodide and fluorescein isothiocyanate ͑FITC͒-Annexin V. The location of the ice front was recorded and a cell death boundary which was different from the ice front was observed. By controlling the cooling process in a microfluidic channel, it is possible to recreate a sketch of biological effect during the process of simulated cryosurgery.

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Mechanisms of intracellular ice formation

Cited by 209 publications

References 17 publications

Stress‐tolerance of baker's‐yeast (Saccharomyces cerevisiae) cells: stress‐protective molecules and genes involved in stress tolerance

Stress‐tolerance of baker's‐yeast (Saccharomyces cerevisiae) cells: stress‐protective molecules and genes involved in stress tolerance

Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

Cell death along single microfluidic channel after freeze-thaw treatments

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