Mechanisms of Ischemic Preconditioning in Rat Myocardium

Vuorinen, Klaus; Ylitalo, Kari; Peuhkurinen, Keijo; Raatikainen, Pekka; Ala-Rämi, Antti; Hassinen, Ilmo E.

doi:10.1161/01.cir.91.11.2810

Cited by 119 publications

(44 citation statements)

References 44 publications

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“…Similarly, oligomycin, which inhibits F 1 F o -ATPase through its F o domain, preserves ATP and protects against or postpones injury during ischemia (72). However, it is difficult to envisage how inhibition of mitochondrial F 1 F o -ATPase by dietary resveratrol and related polyphenols could have a similar effect and so contribute to the cardiovascular protective effects associated with dietary polyphenols.…”

Section: Mechanism Of Inhibitionmentioning

confidence: 99%

Mechanism of inhibition of bovine F ₁ -ATPase by resveratrol and related polyphenols

Gledhill

Montgomery

Leslie

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

357

330

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The structures of F1-ATPase from bovine heart mitochondria inhibited with the dietary phytopolyphenol, resveratrol, and with the related polyphenols quercetin and piceatannol have been determined at 2.3-, 2.4-and 2.7-Å resolution, respectively. The inhibitors bind to a common site in the inside surface of an annulus made from loops in the three ␣-and three ␤-subunits beneath the ''crown'' of ␤-strands in their N-terminal domains. This region of F 1-ATPase forms a bearing to allow the rotation of the tip of the ␥-subunit inside the annulus during catalysis. The binding site is a hydrophobic pocket between the C-terminal tip of the ␥-subunit and the ␤TP subunit, and the inhibitors are bound via H-bonds mostly to their hydroxyl moieties mediated by bound water molecules and by hydrophobic interactions. There are no equivalent sites between the ␥-subunit and either the ␤DP or the ␤E subunit. The inhibitors probably prevent both the synthetic and hydrolytic activities of the enzyme by blocking both senses of rotation of the ␥-subunit. The beneficial effects of dietary resveratrol may derive in part by preventing mitochondrial ATP synthesis in tumor cells, thereby inducing apoptosis. mitochondria ͉ oxidative phosphorylation ͉ rotary mechanism ͉ crystal structure

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Section: Mechanism Of Inhibitionmentioning

confidence: 99%

Mechanism of inhibition of bovine F ₁ -ATPase by resveratrol and related polyphenols

Gledhill

Montgomery

Leslie

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

357

330

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“…The binding pocket for the F1-targeting inhibitor efrapeptin is localized in a, b and g subunits while that for aurovertin B is localized mainly in the b subunit . Inhibition of F0F1-ATPase/ATP synthase by the F0-targeting inhibitor, oligomycin, has been shown to protect or postpone cell injury by preserving ATP during ischaemia (Vuorinen et al, 1995) and to induce apoptosis in tumour cells (Wolvetang et al, 1994). Oligomycin also inhibits the apoptosis induced by Bax, a pro-apoptotic protein localized on the mitochondrial outer membrane, and F0F1-ATPase is required for the killing of cells by Bax (Matsuyama et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of mitochondrial proton F0F1‐ATPase/ATP synthase by polyphenolic phytochemicals

Zheng¹,

Ramírez²

2000

British J Pharmacology

416

289

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1 Mitochondrial proton F0F1-ATPase/ATP synthase synthesizes ATP during oxidative phosphorylation. In this study, we examined the eects of several groups of polyphenolic phytochemicals on the activity of the enzyme. 2 Resveratrol, a stilbene phytoalexin that is present in grapes and red wine, concentrationdependently inhibited the enzymatic activity of both rat brain and liver F0F1-ATPase/ATP synthase (IC 50 of 12 ± 28 mM). 3 Screening of other polyphenolic phytochemicals using rat brain F0F1-ATPase activity resulted in the following ranking potency (IC 50 in parenthesis): piceatannol (8 mM)4resveratrol (19 mM)= (7)epigallocatechin gallate (17 mM)4(7)epicatechin gallate, curcumin (45 mM)4genistein=biocha-nin A=quercetin=kaempferol=morin (55 ± 65 mM)4phloretin=apigenin=daidzein (approx. 100 mM). Genistin, quercitrin, phloridzin, (+)catechin, (+)epicatechin, (7)epicatechin and (7)epigallocatechin had little eect at similar concentrations. Tannic acid, thea¯avins (tea extract) and grape seed proanthocyanidin extract (GSPE) had IC 50 values of 5, 20 and 30 mg ml 71 , respectively. Several monophenolic antioxidants and non-phenolic compounds were ineective at concentrations of 210 mM or higher. 4 The inhibition of F0F1-ATPase by resveratrol and genistein was non-competitive in nature. 5 The eects of polyphenolic phytochemicals were additive. 6 Both resveratrol and genistein had little eect on the Na + /K + -ATPase activity of porcine cerebral cortex, whereas quercetin had similar inhibitory potency as for F0F1-ATPase. 7 In conclusion, the ATP synthase is a target for dietary phytochemicals. This pharmacological property of these phytochemicals should be included in the examination of their health bene®ts as well as potential cytotoxicity.

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“…Так была предложена метаболи-ческая концепция ишемического прекондициониро-вания. Полагают, что энергосберегающие эффекты ИП вызваны снижением активности протонной ми-тохондриальной F 0 F 1 АТФазы, дефосфорилирующей основное количество АТФ при ишемии [111]. На-ряду с этим снижается активность Na…”

Section: Aadsorasunclassified

From the S.P. Botkin’s idea of “preexposure” to preconditioning phenomenon. Perspectives for use of phenomena of ischemic and pharmacological preconditioning

Zarubina

Викторовна²,

Shabanov

et al. 2016

Rev Clin Pharm Drug Ther

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The phenomenon of ischemic preconditioning based on the S.P. Botkin’s idea about defense effect of disturbing factors acting in small intensities is observed in the review. The modern literature data about main types of preconditioning exposure, triggers and mechanisms of ischemic preconditioning are reviewed. This phenomenon was supported in many experiments in vivo and in vitro on animals of different spices as well as in humans in clinical conditions. Ischemic preconditioning is qualified as transient positive changes in the organs and tissues produced by activation of rapid endogenous adoptive processes in them during the short period of subletal ischemia and reperfusion and which defend them from subsequent ischemic episodes. There are early and late ischemic preconditioning (the second window of defense). The first type of ischemic preconditioning belongs to classic type of preconditioning and is produced by the short ischemic episodes (3-5 min) and similar intervals of reperfusion. Ischemic preconditioning observed in a day or more after preconditioning stimuli is named as late preconditioning with genes expression, synthesis of heat shock proteins (HSP 72 in particular) and NO synthase as the basis mechanisms underlying of it. Administration of triggers like adenosine, forbol ether, bradykinine or glycerol derivatives into the blood or ischemic tissues produces defense action similar to ischemic preconditioning and qualified as pharmacological preconditioning. Preconditioning induced by pharmacological agents are more preference than short ischemic episodes. Antihypoxic effects of benzimidazol derivatives in both an acute hypoxia and hypoxic preconditioning are described in the article. Other perspectives of pharmacological preconditioning in practical use are also discussed.

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Mechanisms of Ischemic Preconditioning in Rat Myocardium

Cited by 119 publications

References 44 publications

Mechanism of inhibition of bovine F ₁ -ATPase by resveratrol and related polyphenols

Mechanism of inhibition of bovine F ₁ -ATPase by resveratrol and related polyphenols

Inhibition of mitochondrial proton F0F1‐ATPase/ATP synthase by polyphenolic phytochemicals

From the S.P. Botkin’s idea of “preexposure” to preconditioning phenomenon. Perspectives for use of phenomena of ischemic and pharmacological preconditioning

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Mechanisms of Ischemic Preconditioning in Rat Myocardium

Cited by 119 publications

References 44 publications

Mechanism of inhibition of bovine F 1 -ATPase by resveratrol and related polyphenols

Mechanism of inhibition of bovine F 1 -ATPase by resveratrol and related polyphenols

Inhibition of mitochondrial proton F0F1‐ATPase/ATP synthase by polyphenolic phytochemicals

From the S.P. Botkin’s idea of “preexposure” to preconditioning phenomenon. Perspectives for use of phenomena of ischemic and pharmacological preconditioning

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Mechanism of inhibition of bovine F ₁ -ATPase by resveratrol and related polyphenols

Mechanism of inhibition of bovine F ₁ -ATPase by resveratrol and related polyphenols