Mechanisms of Maturation and Germination in Crop Seeds Exposed to Environmental Stresses with a Focus on Nutrients, Water Status, and Reactive Oxygen Species

Ishibashi, Yoshihiro; Yuasa, Takashi; Iwaya‐Inoue, Mari

doi:10.1007/978-981-13-1244-1_13

Cited by 13 publications

(10 citation statements)

References 161 publications

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“…Rice is the third highest yielding crop after corn and sugarcane (UN Food and Agriculture Organization, 2017). Given its small genome and abundant genetic resources, rice is considered a model monocot (Ishibashi et al, 2018). However, the vitality of rice seeds, especially hybrid rice seeds, decreases easily during natural storage, which has huge adverse effects on food production and economic benefits (Ishibashi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice

et al. 2020

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MicroRNAs (miRNAs) play important roles in various aspects of plant physiology and metabolism. The expression level of miR164c is negatively correlated with seed vigor in rice ( Oryza sativa L.); however, the mechanism of seed vigor regulation by miR164c remains unknown. Anti-aging capacity is an important indicator of seed vigor. Here, we report an miR164c-guided gene/protein interaction network that regulates the anti-aging ability of rice seeds. Seeds of the wild-type (WT) rice cultivar “Kasalath” and its transgenic derivatives, miR164c-silenced line (MIM164c) and miR164c overexpression line (OE164c), with significant differences in anti-aging capacity, showed significant differences in gene and protein expression levels. The differentially expressed genes (DEGs) or proteins were significantly enriched in six metabolic functional categories related to seed vigor, including “stress response,” “protein processing in endoplasmic reticulum (ER),” “embryo development,” “serine-type endopeptidase inhibitor,” “energy metabolism,” and “other.” Differences in the expression levels of genes or proteins related to energy metabolism, serine endopeptidase, and stress response in seeds under normal storage conditions may be associated with anti-aging capacity. The results of gene/protein interaction analyses suggest that miR164c first targets PSK5 , and the PSK5 protein then interacts with the ubiquitin-associated gene RPS27AA , which simultaneously impacts the genes/proteins in the six above-mentioned functional categories. Expression levels of some of the key genes and proteins in the interaction network were verified by real-time fluorescence quantitative PCR (RT-qPCR) and multiple reaction monitoring mass spectrometry (MRM-MS), respectively. Thus, the present study provides new insights into the miRNA-mediated gene and protein interaction network that regulates seed vigor.

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Section: Introductionmentioning

confidence: 99%

Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice

et al. 2020

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“…Remobilization of nutrients and water during seed filling under abiotic stress conditions affects the production of healthy seeds. Environmental stresses caused by drought and temperature extremes reduce grain quality in staple food crops, such as rice ( Oryza sativa ), soybean ( Glycine max ), and wheat ( Triticum aestivum ) (Ishibashi et al ). Dehydrogenase and esterase activity as well as antioxidant potential are positively correlated with seed vigor in rice (Talai and Sen‐Mandi ).…”

Section: Introductionmentioning

confidence: 99%

miR164c and miR168a regulate seed vigor in rice

Zhou

Wang

et al. 2019

JIPB

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MicroRNAs (miRNAs) are key regulators of gene expression in many important biological processes of plants. However, few miRNAs have been shown to regulate seed vigor. Here, we conducted microarray assays to analyze miRNA expression levels in seeds of the rice (Oryza sativa L.) cultivar ZR02. Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds. Among these, osa‐miR164c was transcriptionally upregulated, while osa‐miR168a was downregulated in artificially aged seeds; this was verified by quantitative real‐time PCR analysis. Under the same aging condition, osa‐miR164c overexpression in OE164c transgenic seeds and osa‐miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates, whereas osa‐miR164c silencing in MIM164c and osa‐miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild‐type seeds. Meanwhile, changes in cytomembrane permeability of seeds and in the expression level of osa‐miR164c target genes (OsPM27 and OsPSK5) and osa‐miR168a target genes (OsAGO1 and OsPTR2) under aging conditions coincided with changes in seed vigor induced by osa‐miR164c and osa‐miR168a. Thus, genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds.

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“…It has been reported that water stress causes increased ABA levels and decreased cytokinin, leading to early senescence (Yang et al, 2003). To protect themselves against environmental stresses during maturation, seeds have mechanisms of tolerance to desiccation such as the accumulation of sugars, which stabilize the plasma membranes, liposomes, and proteins (Buitink et al, 2006;Battaglia & Covarrubias, 2013;Ishibashi, Yuasa, & Iwaya-Inoue, 2018). These mechanisms are triggered at the end of the maturation stage and, possibly, the early senescence rate of the plants in this treatment (visually observed) did not allow that the mechanisms of tolerance to desiccation appeared in the seeds produced by the plants cultivated under lower irrigation depths.…”

Section: Resultsmentioning

confidence: 88%

Yield and physiological quality of wheat seeds produced under different irrigation depths and leaf Silicon

Gama

Oliveira

Pinheiro

et al. 2021

Semina: Ciênc. Agrár.

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Water availability is one of the main factors responsible for wheat productivity, as well as the quality of the produced seeds. Silicon (Si) has an important role in mitigating the effects of various biotic and abiotic stresses. Thus, Si application can be used to mitigate the effects of different irrigation depths on the production and quality of wheat seeds. The work aimed to evaluate the yield and physiological quality of wheat seeds produced from plants fertilized with leaf Si and grown under different irrigation depths. The experiment was laid out in a split-plot randomized block design, with four replications. The plots consisted of three irrigation depths (0, 50, and 100% of the total irrigation requirement [TIR]). In the subplots, Si treatments were allocated (without application [0 mM] and 5 mM SiO2, applied at the tillering stage). The following parameters were evaluated: water balance of the system; soil moisture; yield; thousand seed weight; germination; electrical conductivity; accelerated aging; seedling length and emergence. The water balance of the system was negative for the 0% TIN irrigation depth after anthesis and there was less soil moisture in this depth. There was no effect of irrigation depths and Si application on plant yield. The smaller irrigation depths imposed reduced the thousand seed weight and the electrical conductivity of the seeds produced. Plants fertilized with Si did not differ in germination, but they produced more vigorous seedlings with greater growth and uniformity.

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Mechanisms of Maturation and Germination in Crop Seeds Exposed to Environmental Stresses with a Focus on Nutrients, Water Status, and Reactive Oxygen Species

Cited by 13 publications

References 161 publications

Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice

Elucidation of the miR164c-Guided Gene/Protein Interaction Network Controlling Seed Vigor in Rice

miR164c and miR168a regulate seed vigor in rice

Yield and physiological quality of wheat seeds produced under different irrigation depths and leaf Silicon

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