Mechanisms of Myocardial Edema Development in CVD Pathophysiology

Kiseleva, Diana G.; Kirichenko, Tatiana V.; Markina, Yuliya V.; Cherednichenko, Vadim R.; Gugueva, Ekaterina A.; Markin, Alexander M.

doi:10.3390/biomedicines12020465

Cited by 2 publications

References 106 publications

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Arrhythmogenic Potential of Myocardial Edema: The Interstitial Osmolality Induces Spiral Waves and Multiple Excitation Wavelets

Kiseleva,

Dzhabrailov,

Aitova

et al. 2024

Biomedicines

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Myocardial edema is a common symptom of pathological processes in the heart, causing aggravation of cardiovascular diseases and leading to irreversible myocardial remodeling. Patient-based studies show that myocardial edema is associated with arrhythmias. Currently, there are no studies that have examined how edema may influence changes in calcium dynamics in the functional syncytium. We performed optical mapping of calcium dynamics on a monolayer of neonatal rat cardiomyocytes with Fluo-4. The osmolality of the solutions was adjusted using the NaCl content. The initial Tyrode solution contained 140 mM NaCl (1T) and the hypoosmotic solutions contained 105 (0.75T) and 70 mM NaCl (0.5T). This study demonstrated a sharp decrease in the calcium wave propagation speed with a decrease in the solution osmolality. The successive decrease in osmolality also showed a transition from a normal wavefront to spiral wave and multiple wavelets of excitation with wave break. Our study demonstrated that, in a cellular model, hypoosmolality and, as a consequence, myocardial edema, could potentially lead to fatal ventricular arrhythmias, which to our knowledge has not been studied before. At 0.75T spiral waves appeared, whereas multiple wavelets of excitation occurred in 0.5T, which had not been recorded previously in a two-dimensional monolayer under conditions of cell edema without changes in the pacing protocol.

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Arrhythmogenic Potential of Myocardial Edema: The Interstitial Osmolality Induces Spiral Waves and Multiple Excitation Wavelets

Kiseleva,

Dzhabrailov,

Aitova

et al. 2024

Biomedicines

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The Effect of 1%, 2% and 4% Sucrose Induction on Zebrafish Cardiomyocyte Damage

Damayanti,

Martino

2024

8th International Students Science Congress Proceedings Book

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One of the complications of diabetes is cardiovascular disease. Some studies mention insulin resistance in the cadiac muscle will increase the occurrence of inflammation and necrosis of cardiac muscle cells. The study aims to prove the effect of sucrose induction on zebrafish cardiomyocyte damage. The research was conducted in the laboratory, using a post test group control design. The research sample used zebra fish. The research group consisted of normal control group (N) and sucrose induction group 1% (G1), 2% (G2) and 4% (G3). Hyperglycemia induction is a modification of previous researchers. The process of inducing 1%, 2%, 4% sucrose in zebrafish bath water for 28 days which is replaced every 2 days. The treatment process followed laboratory procedures. At the end of the study, zebrafish were fed for 10 hours, then anesthetized by placing the fish in ice water for 10 minutes. Furthermore, blood glucose levels were measured through the tail using glucotest. Normal glucose levels of zebrafish are between 40-90 mg/dL. Cardiac organ was taken and histology preparate was made. Cardiac damage in the form of leukocyte infiltration and the area of cardiac muscle cell edema was calculated histologically, with HE staining, 400 x magnification, using image J software. Measurement data were analyzed using ONEWAY ANOVA, with a significance level of p<0.05. The average percentage of leukocyte cell infiltration of G3 was 40% higher than N (15%), G1 (19%) and G2 (27%) with p<0.05. G3 odem area of (42%) was greater than the normal group N (5%), G1 (18%) and G2 (34%) with p<0.05. It is suspected that this condition is due to the effect of hyperglycemia due to the induction of 4% sucrose (179mg/dL). From this study it can be concluded that 4% glucose induction causes damage to zebrafish heart cells which is characterized by the presence of leukocyte cell inflitration of 40% and odem area of 42%.

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Mechanisms of Myocardial Edema Development in CVD Pathophysiology

Cited by 2 publications

References 106 publications

Arrhythmogenic Potential of Myocardial Edema: The Interstitial Osmolality Induces Spiral Waves and Multiple Excitation Wavelets

Arrhythmogenic Potential of Myocardial Edema: The Interstitial Osmolality Induces Spiral Waves and Multiple Excitation Wavelets

The Effect of 1%, 2% and 4% Sucrose Induction on Zebrafish Cardiomyocyte Damage

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