Mechanisms of resistance to acetolactate synthase‐inhibiting herbicides in populations of <i>Apera spica‐venti</i> from the Czech Republic

Hamouzová, Kateřina; Košnarová, Pavlína; Salava, J.; Soukup, Josef; Hamouz, Pavel

doi:10.1002/ps.3563

Cited by 28 publications

(39 citation statements)

References 31 publications

(48 reference statements)

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“…Similar results were obtained by Krysiak et al (2011) and Hamouzova et al (2014). Th e probable cause of this might be the existence of another mechanism of resistance, for example enhanced metabolism.…”

Section: Discussionsupporting

confidence: 81%

A strategy of chemical control of Apera spica-venti L. resistant to sulfonylureas traced on the molecular level

Stankiewicz‐Kosyl¹,

Wrochna²,

Salas³

et al. 2017

Journal of Plant Protection Research

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Th ree populations of silky bent grass (Apera spica-venti L.) were tested -one that is susceptible and two that are resistant to sulfonylureas. Th is study assessed the effi cacy of control by diff erent herbicides in a pot experiment and estimated the molecular status of resistance to sulfonylureas in analysed populations and its eff ect on the effi cacy of diff erent chemical treatments. Th e three most eff ective herbicide rotation schemes were: 1) chlorsulfuron + isoproturon, ethametsulfuron + metazachlor + quinmerac, chlorsulfuron + isoproturon; 2) prosulfocarb + difl ufenican, ethametsulfuron + quizalofop-p-ethyl, prosulfocarb + diflufenican; 3) difl ufenican + fl ufenacet, quizalofop-p-ethyl, difl ufenican + fl ufenacet. In most cases it was more diffi cult to destroy 100% of the resistant population from Modgarby where the majority of plants had no mutation in the als gene. In the resistant population from Babin there were signifi cantly more individuals with mutation in the als gene, therefore exhibiting target-site resistance.

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Section: Discussionsupporting

confidence: 81%

A strategy of chemical control of Apera spica-venti L. resistant to sulfonylureas traced on the molecular level

Stankiewicz‐Kosyl¹,

Wrochna²,

Salas³

et al. 2017

Journal of Plant Protection Research

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“…Cytochrome monoxygenases (P450s) are one of the largest groups of enzyme families that are responsible for plant tolerance to herbicides (Buker, 2004;Beckie et al, 2012;Yu and Powles, 2013;Hamouzova et al, 2014;Rojano Delgado et al, 2014). The P450 enzymes are present in the endoplasmic reticulum and are involved in synthesis of hormones derived from fatty acids and also with the secondary metabolism of plants (Powles and Yu, 2010).…”

Section: Introductionmentioning

confidence: 99%

Tolerance of Common Bean Plants to Ethoxysulfuron Herbicide and the Mechanism Involved in the Process

et al. 2016

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-The objectives of this study were to determine the effect of herbicide ethoxysulfuron on the development of commom bean (Phaseolus vulgaris), to evaluate the impact of the herbicide on the crop grain yield and to determine the mechanism of tolerance of common bean to ethoxysulfuron. Field experiments were carried out with several doses of ethoxysulfuron to determine its effect on the grain yield of two varieties of common bean ("IPR Andorinha", "IPR Tangará"). The mechanism of plant tolerance was evaluated through several methods, including the analysis of the ALS enzyme sensitivity to herbicide and the use of P450 inhibitors (the insecticides malathion + chlorpyrifos) and the use of antidote (mefenpyr-diethyl). When the rate of ethoxysulfuron was 83.2 g ha -1 , the grain yield loss reached 25% (IPR Tangará) and 35% (IPR Andorinha). The ALS enzyme activity was severely reduced even at low concentrations ethoxysulfuron (1 μM), indicating that the mechanism of tolerance is not the insensitivity of the herbicide target enzyme. The inhibitors of herbicide detoxification increased the sensitivity of the bean plants to ethoxysulfuron, and the antidote (stimulator of herbicide degradation) mitigated the herbicidal effect. These results strongly support the hypothesis that the mechanism of tolerance of common bean plants to ethoxysulfuron is enhanced herbicide detoxification.Keywords: Phaseolus vulgaris, crop grain yield, ALS enzyme, detoxification. RESUMO -Os objetivos deste trabalho foram determinar o efeito do herbicida ethoxysulfuron sobre o desenvolvimento do feijoeiro (Phaseolus vulgaris), avaliar o impacto do herbicida sobre o rendimento de grãos e determinar o mecanismo de tolerância do feijoeiro ao ethoxysulfuron. Em campo, foram realizados dois experimentos com doses crescentes de ethoxysulfuron, a fim de determinar o seu efeito sobre o desenvolvimento e o rendimento de grãos de dois cultivares (cvs.) de feijão (IPR Andorinha e IPR Tangará). O mecanismo de tolerância das plantas foi determinado através da análise de sensibilidade da enzima ALS ao herbicida, do uso de inseticidas inibidores da P450 (malathion + chlorpyrifos) e do uso de antídoto (mefenpyr-diethyl). Quando a dose de ethoxysulfuron foi de 83,2 g ha -1 , as perdas de rendimento de grãos atingiram 25% (IPR Tangará) e 35% (IPR Andorinha

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“…This mutation is widely known to endow cross‐resistance to sulfonylureas and imidazolinones, thus results were consistent with what was previously reported. Trp 574 Met substitution, found in accession 17–56 H of A. hybridus , was previously described in Apera spica‐venti L., however the authors tested chlorsulfuron resistance, but not other ALS‐inhibitors . It is worth mentioning that this amino acid substitution is caused by a double mutation, an overall rare and intriguing event .…”

Section: Discussionmentioning

confidence: 94%

“…Trp 574 Met substitution, found in accession 17-56 H of A. hybridus, was previously described in Apera spica-venti L., however the authors tested chlorsulfuron resistance, but not other ALS-inhibitors. 48 It is worth mentioning that this amino acid substitution is caused by a double mutation, an overall rare and intriguing event. 49,50 Trp 574 Arg, which was detected in only one plant of 17-56 H, had previously been found in Digitaria sanguinalis (L.) Scop.…”

Section: Discussionmentioning

confidence: 99%

A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy

Milani

Scarabel

Sattin

2019

Pest Management Science

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BACKGROUND Several soybean fields in Italy were found to be infested by multiple species of Amaranthus spp. not adequately controlled by acetolactate (ALS) inhibitor herbicides. The objectives of this research were (i) to create a simplified botanical key to identify weedy amaranths; (ii) to determine the number and type of sites of action the accession are resistant to, i.e. resistance pattern; and (iii) to determine the main resistance mechanisms involved d) to evaluate the efficacy of herbicides with different site of action. RESULTS An easy‐to‐use botanical key was devised and successfully used in the infested sites and results were confirmed through a species‐specific molecular marker. Amaranthus retroflexus L. (redrood pigweed) was found in three sites; plants with Asp376Glu substitution at the ALS gene were resistant to thifensulfuron‐methyl. Amaranthus tuberculatus (Moq.) J.D.Sauer (waterhemp) and Amaranthus hybridus L. (smooth pigweed) accessions were cross‐resistant to thifensulfuron‐methyl and imazamox; most ALS‐resistant plants had a point mutation at position 574. One A. hybridus accession had the substitution Trp574Met, new for Amaranthus genus. All ALS‐resistant accessions were controlled by glyphosate and metribuzin. A. retroflexus accessions were controlled by bentazon, instead an A. hybridus and some A. tuberculatus accession were not. CONCLUSIONS The simplified botanical key proposed herein could be a useful tool for farmers and weed scientists to reliably identify Amaranthus species in the field. The main resistance mechanism in the three Amaranthus species is target‐site mediated. This is the first evidence of ALS‐resistant A. tuberculatus outside its native North American range. © 2019 Society of Chemical Industry

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Mechanisms of resistance to acetolactate synthase‐inhibiting herbicides in populations of Apera spica‐venti from the Czech Republic

Abstract: Individuals of Apera spica-venti populations tested in this study possess the target-site ALS resistance mutation and an additional so far unknown resistance mechanism(s).

Cited by 28 publications

References 31 publications

A strategy of chemical control of Apera spica-venti L. resistant to sulfonylureas traced on the molecular level

A strategy of chemical control of Apera spica-venti L. resistant to sulfonylureas traced on the molecular level

Tolerance of Common Bean Plants to Ethoxysulfuron Herbicide and the Mechanism Involved in the Process

A family affair: resistance mechanism and alternative control of three Amaranthus species resistant to acetolactate synthase inhibitors in Italy

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