Mechanisms of uranium mineralization by the yeast Saccharomyces cerevisiae

Ohnuki, Toshihiko; Ozaki, Takuo; Yoshida, Takahiro; Sakamoto, Fuminori; Kozai, Naofumi; Wakai, Eiichi; Francis, A.J.; Iefuji, Haruyuki

doi:10.1016/j.gca.2005.06.023

Cited by 54 publications

(29 citation statements)

References 36 publications

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“…7A), also suggesting that U adsorption onto the bacteria does not control U concentrations in the higher saturation state experiments. This behavior is not a result of increased saturation state conditions in biotic experiments, since higher saturation states would result in less U remaining in solution in the biotic experiments compared to the abiotic controls (Ohnuki et al, 2005).…”

Section: Icp-oesmentioning

confidence: 93%

The effects of non-metabolizing bacterial cells on the precipitation of U, Pb and Ca phosphates

Dunham‐Cheatham

Xue

Bunker

et al. 2011

Geochimica et Cosmochimica Acta

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In this study, we test the potential for passive cell wall biomineralization by determining the effects of non-metabolizing bacteria on the precipitation of uranyl, lead, and calcium phosphates from a range of over-saturated conditions. Experiments were performed using Gram-positive Bacillus subtilis and Gram-negative Shewanella oneidensis MR-1. After equilibration, the aqueous phases were sampled and the remaining metal and P concentrations were analyzed using inductively coupled plasmaoptical emission spectroscopy (ICP-OES); the solid phases were collected and analyzed using X-ray diffractometry (XRD), transmission electron microscopy (TEM), and X-ray absorption spectroscopy (XAS).At the lower degrees of over-saturation studied, bacterial cells exerted no discernable effect on the mode of precipitation of the metal phosphates, with homogeneous precipitation occurring exclusively. However, at higher saturation states in the U system, we observed heterogeneous mineralization and extensive nucleation of hydrogen uranyl phosphate (HUP) mineralization throughout the fabric of the bacterial cell walls. This mineral nucleation effect was observed in both B. subtilis and S. oneidensis cells. In both cases, the biogenic mineral precipitates formed under the higher saturation state conditions were significantly smaller than those that formed in the abiotic controls.The cell wall nucleation effects that occurred in some of the U systems were not observed under any of the saturation state conditions studied in the Pb or Ca systems. The presence of B. subtilis significantly decreased the extent of precipitation in the U system, but had little effect in the Pb and Ca systems. At least part of this effect is due to higher solubility of the nanoscale HUP precipitate relative to macroscopic HUP. This study documents several effects of non-metabolizing bacterial cells on the nature and extent of metal phosphate precipitation. Each of these effects likely contributes to higher metal mobilities in geologic media, but the effects are not universal, and occur only with some elements and only under a subset of the conditions studied.

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Section: Icp-oesmentioning

confidence: 93%

The effects of non-metabolizing bacterial cells on the precipitation of U, Pb and Ca phosphates

Dunham‐Cheatham

Xue

Bunker

et al. 2011

Geochimica et Cosmochimica Acta

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“…During past few decades, biosorption has been studied extensively using various biomasses such as white-rot fungus [5][6][7], Saccharomyces cerevisiae [8,9], Cladosporium sp. [10], Bacillus subtilis [11,12] and Chlorella sorokiniana [13,14].…”

Section: Introductionmentioning

confidence: 99%

Kinetic studies for the biosorption of lead and copper ions by Penicillium simplicissimum immobilized within loofa sponge

Liao

et al. 2008

Journal of Hazardous Materials

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“…Examination of the surfaces of biomineralized uraniferous hydrocarbons showed biogenic filaments resembling fungi or actinomycetes (Milodowski et al, 1990). A yeast strain of Saccharomyces cerevisiae also showed some features of uranium biomineralization via formation of uranyl phosphate minerals during growth in a medium amended with high concentration of phosphate (Ohnuki et al, 2005). The first detailed reports for uranium biomineralization by fungi showed that saprotrophic, ericoid and ectomycorrhizal fungi could solubilize uranium oxides (UO3 and U3O8), and accumulated uranium within the mycelium to over 80 mg/g dry wt -1 , most of which was biomineralized as well-crystallized uranyl phosphate minerals of the meta-autunite group.…”

Section: Mechanisms and Microbesmentioning

confidence: 97%