Mechanistic insight into the rapid one-step facile biofabrication of antibacterial silver nanoparticles from bacterial release and their biogenicity and concentration-dependent in vitro cytotoxicity to colon cells

Verma, Suresh K.; Jha, Ealisha; Sahoo, Babrubahan; Panda, Pritam Kumar; Thirumurugan, Arun; Parashar, S. K. S.; Suar, Mrutyunjay

doi:10.1039/c7ra05943d

Cited by 72 publications

(31 citation statements)

References 63 publications

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“…The main components of the cell membrane are proteins and lipids, and AgNPs can interact with proteins to form complexes with substances containing oxygen, phosphorus, sulfur or nitrogen atoms [35, 36]. Studies have shown that the reaction of AgNPs with sulfur-containing membrane proteins may lead to inactivation of membrane-bound enzymes and proteins [14, 35]. AgNPs can also interfere with the respiratory chain and reduce energy production when attacking the membrane [13].…”

Section: Resultsmentioning

confidence: 99%

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Zhang

et al. 2018

PLoS ONE

140

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The influence of nanomaterials on the ecological environment is becoming an increasingly hot research field, and many researchers are exploring the mechanisms of nanomaterial toxicity on microorganisms. Herein, we studied the effect of two different sizes of nanosilver (10 nm and 50 nm) on the soil nitrogen fixation by the model bacteria Azotobacter vinelandii. Smaller size AgNPs correlated with higher toxicity, which was evident from reduced cell numbers. Flow cytometry analysis further confirmed this finding, which was carried out with the same concentration of 10 mg/L for 12 h, the apoptotic rates were20.23% and 3.14% for 10 nm and 50 nm AgNPs, respectively. Structural damage to cells were obvious under scanning electron microscopy. Nitrogenase activity and gene expression assays revealed that AgNPs could inhibit the nitrogen fixation of A. vinelandii. The presence of AgNPs caused intracellular reactive oxygen species (ROS) production and electron spin resonance further demonstrated that AgNPs generated hydroxyl radicals, and that AgNPs could cause oxidative damage to bacteria. A combination of Ag content distribution assays and transmission electron microscopy indicated that AgNPs were internalized in A. vinelandii cells. Overall, this study suggested that the toxicity of AgNPs was size and concentration dependent, and the mechanism of antibacterial effects was determined to involve damage to cell membranes and production of reactive oxygen species leading to enzyme inactivation, gene down-regulation and death by apoptosis.

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Section: Resultsmentioning

confidence: 99%

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Zhang

et al. 2018

PLoS ONE

140

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“…The literature revealed that nanoparticle challenge triggers the cellular oxidative stress. 48,200,201 In order to overcome this stress, cells exhibit protective responses which include enzymatic or nonenzymatic defence mechanisms. [201][202][203] When the oxidative stress overcomes the defence mechanisms, the cell wall and biomolecules such as proteins, lipids, and DNA are subjected to damage caused by ROS and free radicals such as hypochlorous acid (HOCl), hydrogen peroxide (H 2 O 2 ), hydroxyl radical ($OH), superoxide anion (O 2 À ) and singlet oxygen ( 1 O 2 ).…”

Section: Oxidative Stressmentioning

confidence: 99%

Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

et al. 2019

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“…Although, oxidative stress is a normal cellular process that occurs in several phases of cellular signaling however, extreme oxidative stress can be detrimental. Literature has showed that MNPs can trigger cellular oxidative stress [100][101][102]. These free radicals are either in the form of reactive oxygen species (ROS) or reactive nitrogen species (RNS).…”

Section: Free Radical Generationmentioning

confidence: 99%

Bactericidal Antibacterial Mechanism of Plant Synthesized Silver, Gold and Bimetallic Nanoparticles

Fanoro

Oluwafemi

2020

Pharmaceutics

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As the field of nanomedicine develops and tackles the recent surge in antibiotic resistance, there is a need to have an in-depth understanding and a synergistic view of research on the effectiveness of a metal nanoparticle (NP) as an antibacterial agent especially their mechanisms of action. The constant development of bacterial resistance has led scientists to develop novel antibiotic agents. Silver, gold and its bimetallic combination are one of the most promising metal NPs because they show strong antibacterial activity. In this review we discuss the mode of synthesis and the proposed mechanism of biocidal antibacterial activity of metal NPs. These mechanisms include DNA degradation, protein oxidation, generation of reactive oxygen species, lipid peroxidation, ATP depletion, damage of biomolecules and membrane interaction.

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Mechanistic insight into the rapid one-step facile biofabrication of antibacterial silver nanoparticles from bacterial release and their biogenicity and concentration-dependent in vitro cytotoxicity to colon cells

Abstract: Rapid biosynthesis of silver nanoparticles from Gram +ve and Gram −ve bacterial strains and their hiogenicity dependent antibacterial and cytntoxicity.

Cited by 72 publications

References 63 publications

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Size-dependent cytotoxicity of silver nanoparticles to Azotobacter vinelandii: Growth inhibition, cell injury, oxidative stress and internalization

Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity

Bactericidal Antibacterial Mechanism of Plant Synthesized Silver, Gold and Bimetallic Nanoparticles

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