Mechanistic insight of SARS-CoV-2 infection using human hepatobiliary organoids

Zhao, Yi; Ren, Xiaoxue; Lu, Jing; He, Minghui; Liu, Zhe; Liu, Yi; Huang, Mingle; Xiao, Haipeng; Sung, Joseph J.Y.; Li, Xiao Xing; Xu, Lixia; Yu, Jun

doi:10.1136/gutjnl-2021-326617

Cited by 8 publications

(13 citation statements)

References 5 publications

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“…11 Similarly, Zhao et al revealed activated TNF signalling and apoptosis pathways in the infected liver and biliary organoids, implying that SARS-CoV-2 infection might trigger cell death of hepatocytes and cholangiocytes (Figure 5B). 89 A subset of human-specific ACE2 + /TMPRSS2 + cholangiocytes was identified in human liver ductal organoids, thus the organoids were extremely susceptible to SARS-CoV-2, leading to robust viral replication was detected in organoids. 104…”

Section: Liver Organoidsmentioning

confidence: 97%

Development trends of human organoid‐based COVID‐19 research based on bibliometric analysis

Yuan

Zou

et al. 2023

Cell Proliferation

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Coronavirus disease 2019 (COVID‐19), a global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), has posed a catastrophic threat to human health worldwide. Human stem cell‐derived organoids serve as a promising platform for exploring SARS‐CoV‐2 infection. Several review articles have summarized the application of human organoids in COVID‐19, but the research status and development trend of this field have seldom been systematically and comprehensively studied. In this review, we use bibliometric analysis method to identify the characteristics of organoid‐based COVID‐19 research. First, an annual trend of publications and citations, the most contributing countries or regions and organizations, co‐citation analysis of references and sources and research hotspots are determined. Next, systematical summaries of organoid applications in investigating the pathology of SARS‐CoV‐2 infection, vaccine development and drug discovery, are provided. Lastly, the current challenges and future considerations of this field are discussed. The present study will provide an objective angle to identify the current trend and give novel insights for directing the future development of human organoid applications in SARS‐CoV‐2 infection.

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Section: Liver Organoidsmentioning

confidence: 97%

Development trends of human organoid‐based COVID‐19 research based on bibliometric analysis

Yuan

Zou

et al. 2023

Cell Proliferation

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“…In vitro studies have demonstrated that human bile duct organoids are susceptible to SARS-CoV-2 and support viral replication. [ 72 , 73 ] Furthermore, primary hepatocytes and cholangiocytes in culture and organoids infected with SARS-CoV-2 overexpress pro-inflammatory cytokines and downregulate metabolic processes, [ 64 ] raising the possibility that infection may alter the profile of pro-inflammatory or pro-fibrogenic cytokines. Additionally, there may be impairment of bile transport and bile acid signaling, in part from downregulation of bile acid transporters and chloride channels.…”

Section: Mechanisms Of Gastrointestinal and Hepatobiliary Injurymentioning

confidence: 99%

COVID-19: gastrointestinal and hepatobiliary manifestations

Shih

Misdraji

2023

Human Pathology

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“…155,163,164 Importantly, a significant number of SARS-CoV-2 patients with severe symptoms were also reported to have abnormal liver enzyme levels. 164,165 To further understand how the liver could be affected by the SARS-CoV-2 virus, multiple groups have employed the ICO culture [157][158][159] and PSC-derived organoid culture methods 155,156 to validate of direct virus infection in hepatocytes and cholangiocytes, as well as to investigate disease manifestations in the individual cells (Table 3). The discovery of ACE2 and TMPRSS as the proteins mediating SARS-CoV-2 tropism prompted scientists to identify other potential cell types that the virus could infect from single-cell tissue profiling experiments.…”

Section: Other Virus-driven Liver Disease Studies With Organoid Modelsmentioning

confidence: 99%

A decade of liver organoids: Advances in disease modeling

Liu¹,

Sheng²,

Ding³

et al. 2023

Clin Mol Hepatol

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Liver organoids are 3D cellular models of tissue in which cells interact to form unique structures in culture. Since their inception, liver organoids with various cellular compositions, structural features, and functional properties have been described over the past ten years. Methods to create these advanced human cell models range from simple tissue culture techniques to complex bioengineering approaches. These liver organoid culture platforms have been utilized in various liver research fields, including the modeling of liver diseases to regenerative therapy. This review will discuss how liver organoids are used to model diseases, including hereditary liver diseases, primary liver cancer, viral hepatitis, and nonalcoholic fatty liver disease. Specifically, we will focus on studies that utilized two widely adopted approaches: differentiation from pluripotent stem cells and epithelial organoids cultured from patient tissues. These approaches have enabled the generation of advanced human liver models and, more importantly, the establishment of patient-tailored models for evaluating individual-specific disease phenotypes and therapeutic responses.

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Mechanistic insight of SARS-CoV-2 infection using human hepatobiliary organoids

Cited by 8 publications

References 5 publications

Development trends of human organoid‐based COVID‐19 research based on bibliometric analysis

Development trends of human organoid‐based COVID‐19 research based on bibliometric analysis

COVID-19: gastrointestinal and hepatobiliary manifestations

A decade of liver organoids: Advances in disease modeling

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