Mechano‐cytoskeleton remodeling mechanism and molecular docking studies on nanosurface technology: Titania nanotube arrays

Abstract

In biomedical implant technology, nanosurface such as titania nanotube arrays (TNA) could provide better cellular adaptation, especially for long‐term tissue acceptance response. Mechanotransduction activities of TNA nanosurface could involve the cytoskeleton remodeling mechanism. However, there is no clear insight into TNA mechano‐cytoskeleton remodeling activities, especially computational approaches. Epithelial cells have played critical interface between biomedical implant surface and tissue acceptance, particularly for long‐term interaction. Therefore, this study investigates genomic responses that are responsible for cell–TNA mechano‐stimulus using epithelial cells model. Findings suggested that cell–TNA interaction may improve structural and extracellular matrix (ECM) support on the cells as an adaptive response toward the nanosurface topography. More specifically, the surface topography of the TNA might improve the cell polarity and adhesion properties via the interaction of the plasma membrane and intracellular matrix responses. TNA nanosurface might engross the cytoskeleton remodeling activities for multidirectional cell movement and cellular protrusions on TNA nanosurface. These observations are supported by the molecular docking profiles that determine proteins' in silico binding mechanism on TNA. This active cell‐surface revamping would allow cells to adapt to develop a protective barrier toward TNA nanosurface, thus enhancing biocompatibility properties distinctly for long‐term interaction. The findings from this study will be beneficial toward nano‐molecular knowledge of designing functional nanosurface technology for advanced medical implant applications.

Regulation of Tendon Stem Cell Behavior by Designed Nanoporous Topography of Microfibers

“…Despite the extensive work that has been carried out, the challenge we face is to pay attention to cellular mechanisms, integrating various key mechanical induction modules into a complete model to reveal deeper mechanisms. This is similar to how cells integrate different components to coordinate cohesive responses, , as shown in Figure . Many studies have focused on the modules of the topography-induced stem cell differentiation mechanism.…”