Mechanobiology of mesenchymal stem cells: Perspective into mechanical induction of MSC fate

Hao, Jin; Zhang, Yueling; Jing, Dian; Shen, Yun-Dun; Tang, Ge; Huang, Shishu; Zhao, Zhihe

doi:10.1016/j.actbio.2015.04.008

Cited by 156 publications

(117 citation statements)

References 85 publications

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“…[11] Cell shape is a powerful regulator of cell physiology, and stem cell destiny can be influenced by artificially controlling ExtraCellular Matrix (ECM) composition. [12][13][14][15] Indeed, in vitro modulation of topographical features influences cell functions likewise to that provided by the ECM geometry in vivo. [16,17] In addition, topographic surfaces with nanoscale features are able to induce changes in cell polarization, elongation, migration, proliferation, and gene expression.…”

Section: Introductionmentioning

confidence: 95%

Non-Washed Resorbable Blasting Media (NWRBM) on Titanium Surfaces could Enhance Osteogenic Properties of MSCs through Increase of miRNA-196a And VCAM1

Gardin

Ferroni

Piattelli

et al. 2016

Stem Cell Rev and Rep

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Surface topography of Titanium (Ti) dental implants strongly influences osseointegration. In the present work, we have analyzed the influence of two Ti implant surfaces characterized by similar microtopography but different nanotopography and chemistry on the osteoblastic phenotype of Dental Pulp Stem Cells (DPSCs). The effect on osteogenic differentiation, extracellular matrix (ECM) and cell adhesion molecules production have been evaluated by means of molecular biology analyses. The morphology of the cells grown onto these surfaces has been analyzed with SEM and immunofluorescence (IF), and the safety of the surfaces has been tested by using karyotype analysis, Ames test and hemocompatibility assay. Results showed that starting from 15 days of DPSCs culture, a substantial expression of osteoblast specific markers and a strong increase of cell adhesion molecules can be detected. In particular, when DPSCs are seeded on the Ti implants expression of microRNA (miRNA)-196a, which is involved in osteoblastic commitment of stem cells, and of Vascular Cell Adhesion Molecule 1 (VCAM1), a factor involved in angiogenesis, is strongly enhanced.

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Section: Introductionmentioning

confidence: 95%

Non-Washed Resorbable Blasting Media (NWRBM) on Titanium Surfaces could Enhance Osteogenic Properties of MSCs through Increase of miRNA-196a And VCAM1

Gardin

Ferroni

Piattelli

et al. 2016

Stem Cell Rev and Rep

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“…Furthermore, the synthesis and secretion of the ECM is regulated by mechanical stimulation through transcription, post-translational modification, etc. [24][25][26]. Zhang et al found that cyclic mechanical stimulation promoted migration but inhibited the invasion of rat bone marrow stromal cells [27].…”

Section: Wwwimpactjournalscom/oncotarget/ Oncotarget Advance Publimentioning

confidence: 99%

Mechanical stretching promotes the differentiation of BMSC into pelvic floor ligament fibroblasts

Zhao¹,

Wang²,

Ren³

et al. 2018

Oncotarget

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Pelvic floor dysfunction (PFD) is a prevalent and debilitating condition in agingwomen that is associated with weakened pelvic connective tissue. Fibroblasts are the main component of pelvic connective tissue and play a vital role in the maintenance of the pelvic organ. Mechanical stretching is a known modulator leading to the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). In this paper, we measured the lysyl oxidase (LOX), elastin and KDM4B expression in cultured BMSCs under different cyclic mechanical stretch (CMS) conditions via Western blot assays. Our findings indicated that the expression of these proteins was significantly higher in the CMS group than in the normal control group, and 10% CMS at a 0.5 Hz frequency had the best stimulatory effect. We further evaluated the influence of KDM4B on LOX and elastin expression via knockdown and overexpression of KDM4B in BMSCs. Chromatin immunoprecipitation (ChIP)-PCR analysis was performed to determine how H3K9me3 and KDM4B affect the regulatory regions of LOX and elastin in BMSCs subjected to 10% CMS combined with KDM4B knockdown. Finally, we verified the expression of KDM4B under CMS in vivo by subcutaneously transplanting KDM4B-overexpressing BMSCs into mice via immunofluorescence assays. The results showed that activating KDM4B by mechanical stretching increased the protein and gene expression levels of LOX and elastin and induced more LOX-positive cells in the BMSCs. These data suggest that CMS contributes to the differentiation of mesenchymal stem cells (MSCs) into fibroblasts, which indicates its potential use as a cell-based therapy for PFD.

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“…Further, these mechanostatic forces are responsible for large number of transcriptional gene regulations affecting the progression or repair of the tissue pathology. Many studies have explained the link between the mechanical stimuli and the Ca 2+ homeostasis [56,57].…”

Section: Mechanism Behind the Mesenchymal Stem Cell Repairmentioning

confidence: 99%

Mesenchymal Stem Cells: A Future Option for Intervening Disease Management

Chandramoorthy¹,

Radhakrishnan²,

Gurusamy³

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Regeneration, revitalizing and reversal (RRR) are the primordial functions of the stem cells in the field of regenerative medicine. Though there are several cases of successful stem cell transplantation the reversal of metabolic diseases and the acquired secondary complications like chronic renal failure, neuropathy, stroke or vascular diseases are not well studied. The transplanted cells in many cases failed to home or graft in the host with no reason to attribute for such failures. Therefore, it becomes necessary to address these secondary complications with cellular therapy. The oxidative stress of the cells and tissues are attributed to the hostile microenvironment, not suitable for the survival of newly recruited cells. From our few animal studies and published literatures sources elsewhere, we foresee a huge potential for using mesenchymal stem cells (MSCs) to initially combat the secondary cardiovascular and neuronal complications in the management of the metabolic diseases. However, not all the stem cells have been tested in these lines, and further we do not know, whether all the progenitor cells from various sources and origin will behave like MSCs, which needs to be studied extensively.

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Mechanobiology of mesenchymal stem cells: Perspective into mechanical induction of MSC fate

Cited by 156 publications

References 85 publications

Non-Washed Resorbable Blasting Media (NWRBM) on Titanium Surfaces could Enhance Osteogenic Properties of MSCs through Increase of miRNA-196a And VCAM1

Non-Washed Resorbable Blasting Media (NWRBM) on Titanium Surfaces could Enhance Osteogenic Properties of MSCs through Increase of miRNA-196a And VCAM1

Mechanical stretching promotes the differentiation of BMSC into pelvic floor ligament fibroblasts

Mesenchymal Stem Cells: A Future Option for Intervening Disease Management

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