Mechanoresponsive, proteolytically stable and biocompatible supergelators from ultra short enantiomeric peptides with sustained drug release propensity

Abstract

This report describes the discovery of a set of decanoic acid based amphiphilic derivatives that serves as a template for the stabilization of hydrogel nanoparticles for the sustained release of model drugs.

Chiral Orchestration: A Tool for Fishing Out Tripeptide-Based Mechanoresponsive Supergelators Possessing Anti-Inflammatory and Antimicrobial Properties

“…Next, we verified the mechanoresponsive nature of the hydrogels through a series of step-strain measurements, keeping the gelation concentration fixed for all hydrogelators. − …”

“…The dose-dependent cell viability experiments by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the HEK 293 (human embryonic kidney cell) cell line were conducted by incubating the latter for 48 h as shown in Figure A and Table S3 . The IC 50 values of Hydrogelators I – IV were found to be 252.2, 281.9, 242.2, and 296.4 μg/mL, respectively, which were considered to be safe for biomedical applications. , Also, the hydrogelators showed negligible hemolysis at MIC for all of the microorganisms.…”

“…The in vitro antimicrobial activities of all of the hydrogelators were performed against the reported microorganisms by optical density methods as described in ref . For this study, we used 10 dilutions.…”

“…Smart and selective therapeutic approaches derived from stimuli-responsive peptide-based hydrogels invite immense interest from diverse segments of science. − Cost-effectiveness, biocompatibility, control over structural diversity, and involvement of noncovalent interactions have propelled the idea of harnessing the process of self-assembly of peptides for tackling various aspects related to biomedical applications. − Commonly, the stimuli that nucleate the self-assembly phenomena are heat, light, pH, ionic strength, ultrasound, enzymes, and others. − However, these stimuli often take the back seat as they hamper the inherent properties of the molecules due to the utilization of high energy or foreign chemicals. However, exploration of mechanoresponsive hydrogels still remains in its infancy. − In particular, mechanoresponsive (thixotropic) hydrogels are advantageous over others owing to the ease of sol–gel transition commenced simply through mechanical force initiation . The advantage of the designed mechanoresponsive peptide-based therapeutics lies in the fact that all of the properties optimum for biomedical applications are embedded under one roof, which make them ideal candidates to be injected directly, as well of needed.…”

Amine-Derivatized l-Phenylalanine and l-Tyrosine as Versatile Self-Assembled Platforms of Diverse Supramolecular Architectures: From Mesocrystals to Organogels

“…71 On the other hand, due to the syneresis, it was difficult to determine accurately the T gel (temperature above which the gel material lost its structural integrity). For these gels, this volume phase-transition is thermally responsive, and thus, it could be related with potential applications in future investigations, such as in the development of drug delivery systems, 72 sensors, 73 or for the removal of waste contaminants. 74 Morphological and Structural Characterization of the Gels.…”