Mechanosensitive ion channels in cell migration

Coutiño, Brenda Canales; Mayor, Roberto

doi:10.1016/j.cdev.2021.203683

Cited by 36 publications

(25 citation statements)

References 148 publications

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“…The ultrasound and piezoelectric stimulations that lead to cell migration may involve signaling of mechanosensitive ion channels in addition to focal adhesion signaling [ 43 ]. The ultrasound effects on different tissues, such as bone packed with densely-aligned type I collagen and other soft tissues such as brain will involve different intensities of piezoelectricity.…”

Section: Discussionmentioning

confidence: 99%

Piezoelectric effect stimulates the rearrangement of chondrogenic cells and alters ciliary orientation via atypical PKCζ

Lim

Liu

Chu

et al. 2022

Biochemistry and Biophysics Reports

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Section: Discussionmentioning

confidence: 99%

Piezoelectric effect stimulates the rearrangement of chondrogenic cells and alters ciliary orientation via atypical PKCζ

Lim

Liu

Chu

et al. 2022

Biochemistry and Biophysics Reports

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“…Piezo1 is a transmembrane ion channel, exclusively activated by mechanical forces in biological systems (Coste et al, 2010;Lin et al, 2019). Upon Piezo1 activation, calcium ions enter the cell triggering a biochemical cascade, and thereby linking mechanical forces with complex biochemical signalling (Canales Coutiño and Mayor, 2021). Here, we identify Piezo1 as an important mechanosensitive channel in Xenopus migratory NC cells.…”

Section: Development • Accepted Manuscriptmentioning

confidence: 87%

The mechanosensitive channel Piezo1 cooperates with semaphorins to control neural crest migration

Coutiño

Mayor

2021

Development

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Cells are permanently exposed to a multitude of different kinds of signals: however, how cells respond to simultaneous extracellular signals within a complex in vivo environment is poorly understood. Here, we studied the role of the mechanosensitive ion channel Piezo1 on the migration of the neural crest, a multipotent embryonic cell population. We identify that Piezo1 is required for the migration of Xenopus cephalic neural crest. We show that loss of Piezo1 promotes focal adhesion turnover and cytoskeletal dynamics by controlling Rac1 activity, leading to increased speed of migration. Moreover, overactivation of Rac1, due to Piezo1 inhibition, counteracts cell migration inhibitory signals by Semaphorin 3A and Semaphorin 3F, generating aberrant neural crest invasion in vivo. Thus, we find that, for directional migration in vivo, neural crest cells require a tight regulation of Rac1, by semaphorins and Piezo1. We reveal here that a balance between a myriad of signals through Rac1 dictates cell migration in vivo, a mechanism that is likely to be conserved in other cell migration processes.

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“…However, the collagen-induced apoptotic response in RMS cells is both 3D and DDR1-mediated adhesion-dependent. This synergy results from both DDR1-independent pro-apoptotic signals and the activation of DDR1 by the mechanosensitive ion channel TRPV4, which is activated by mechanical confinement as a result of cell membrane stretching ( 31, 36 ). Consequently, the mechanical confinement in 3D cultures results in enhanced pro-apoptotic signals leading to RMS cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Mechanical confinement and DDR1 signalling synergise to regulate collagen-induced apoptosis in rhabdomyosarcoma cells

Gonzalez‐Molina

Kirchhof

Rathod

et al. 2022

Preprint

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Fibrillar collagens promote cell proliferation, migration, and survival in various epithelial cancers and are generally associated with tumour aggressiveness. However, the impact of fibrillar collagens on soft tissue sarcoma behaviour remains poorly understood.Unexpectedly, we find here that fibrillar collagen-related gene expression is associated with favourable patient prognosis in rhabdomyosarcoma. By developing and using collagen matrices with distinct stiffness and in vivo-like microarchitectures, we uncover that the activation of DDR1 has pro-apoptotic and integrin β1 pro-survival function, specifically in 3D rhabdomyosarcoma cell cultures. We demonstrate that rhabdomyosarcoma cell-intrinsic or extrinsic matrix remodelling promotes cell survival. Mechanistically, we find that the 3D-specific collagen-induced apoptosis results from a dual DDR1-independent and a synergistic DDR1-dependent TRPV4-mediated response to mechanical confinement. Altogether, our results indicate that dense microfibrillar collagen-rich microenvironments are detrimental to rhabdomyosarcoma cells through an apoptotic response orchestrated by the induction of DDR1 signalling and mechanical confinement. This mechanism helps to explain the preference of rhabdomyosarcoma cells to grow in and metastasise to low fibrillar collagen microenvironments such as the lung.

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Mechanosensitive ion channels in cell migration

Cited by 36 publications

References 148 publications

Piezoelectric effect stimulates the rearrangement of chondrogenic cells and alters ciliary orientation via atypical PKCζ

Piezoelectric effect stimulates the rearrangement of chondrogenic cells and alters ciliary orientation via atypical PKCζ

The mechanosensitive channel Piezo1 cooperates with semaphorins to control neural crest migration

Mechanical confinement and DDR1 signalling synergise to regulate collagen-induced apoptosis in rhabdomyosarcoma cells

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