Mechanosensitivity and Mechanotransduction

Kamkin, Andre; Kiseleva, Irina

doi:10.1007/978-90-481-9881-8

Cited by 8 publications

(1 citation statement)

References 21 publications

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“…Furthermore, Foster resonance energy transfer (FRET) experiments often limit the number of interactions, the range of extensions and forces that can be explored [24]. In silico Molecular Dynamics models are limited to a simulated time which is in the order of 100 nanoseconds [25], while continuous approaches lack the possibility to consider discrete and highly heterogeneous systems that can form local recurrent structures among the molecules [26].…”

Section: Introductionmentioning

confidence: 99%

Revealing hidden information in osteoblast’s mechanotransduction through analysis of time patterns of critical events

et al. 2020

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Background: Mechanotransduction in bone cells plays a pivotal role in osteoblast differentiation and bone remodelling. Mechanotransduction provides the link between modulation of the extracellular matrix and intracellular actions. By controlling the balance between the intracellular and extracellular domains, the mechanotransduction process determines optimal functionality of the skeletal dynamics, and it is one of the possible causes of osteophatological diseases. Results: Mechanotransduction in a single osteoblast under external mechanical perturbations has been modelled in the agent based framework to reproduce the dynamics of the stochastic reaction diffusion process among molecules in the cytoplasm, nuclear and extracellular domains. The amount of molecules and fluctuations of each molecular class has been analysed in terms of recurrences of critical events. A numerical approach has been developed to invert subordination processes and to extract the directing processes from the molecular signals in order to derive the distribution of recurrence of events. Conclusions:We observed large fluctuations enclose information hidden in the noise which is beyond the dynamic variations of molecular baselines. Studying the system under different parametric conditions and stimuli, the results have shown that the waiting time distributions of each molecule are a signature of the system's dynamics. The behaviours of the molecular waiting times change with the changing of parameters presenting the same variation of patterns for similar interacting molecules and identifying specific alterations for key molecules in the mechanotransduction pathway.

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Section: Introductionmentioning

confidence: 99%

Revealing hidden information in osteoblast’s mechanotransduction through analysis of time patterns of critical events

et al. 2020

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Nanoporous Gold Biointerfaces: Modifying Nanostructure to Control Neural Cell Coverage and Enhance Electrophysiological Recording Performance

et al. 2016

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Nanostructured neural interface coatings have significantly enhanced recording fidelity in both implantable and in vitro devices. As such, nano-porous gold (np-Au) has shown promise as a multifunctional neural interface coating due, in part, to its ability to promote nanostructure-mediated reduction in astrocytic surface coverage while not affecting neuronal coverage. The goal of this study is to provide insight into the mechanisms by which the np-Au nanostructure drives the differential response of neurons versus astrocytes in an in vitro model. Utilizing microfabricated libraries that display varying feature sizes of np-Au, it is demonstrated that np-Au influ-ences neural cell coverage through modulating focal adhesion formation in a feature size-dependent manner. The results here show that surfaces with small (≈30 nm) features control astrocyte spreading through inhibition of focal adhesion formation, while surfaces with large (≈170 nm and greater) features control astrocyte spreading through other mechanotransduction mechanisms. This cellular response combined with lower electrical impedance of np-Au electrodes significantly enhances the fidelity and stability of electrophysiological recordings from cortical neuronglia co-cultures relative to smooth gold electrodes. Finally, by leveraging the effect of nanostructure on neuronal versus glial cell attachment, the use of laser-based nanostructure modulation is demonstrated for selectively patterning neurons with micrometer spatial resolution.

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Mechanical Stretch and Cytokines

Henrotin

Pesesse

Sanchez

2012

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The book "Mechanosensitivity in Cells and Tissues: Mechanical Stretch and Cytokines" edited by Andre Kamkin and Irina Kiseleva impressively demonstrates the diversity of cellular effects of cytokines depending on mechanical stimuli. Clinical and fundamental investigation of biological effects of cytokines in regulation of functioning of cells, tissues and organs is a relatively new, interesting and fast growing field, which draws a lot of attention recently. A proposed link between cytokines, their production and mechanisms of action, and their impact on effects of cellular and tissue stretch given a new momentum to this field. The role of cytokines as regulators of stretch related mechanisms is of special importance since mechanosensitivity plays an important role in a wide variety of biological processes. It is known that cytokines can be marked out as a new separate regulatory system which, along with nervous and endocrine systems, helps to maintain homeostasis, and these three systems are tightly interconnected and interdependent. Presently two major approaches to investigation of cytokines and mechanosensitivity exist. The first one focuses on the effects of interplay of mechanical stimulation of tissue or cells and cytokine production. The second approach investigates the influence of cytokines application on mechanosensitivity and mechanotransduction. This Volume is devoted to those two issues.To my knowledge Andre Kamkin and Irina Kiseleva present the first book on this topic, which consists of a collection of publications, written by leading scientists in this field, which guaranties that this Volume will remain of great interest to readers for a long period of time. Present book is devoted to discussion of the latest findings in the filled of cytokines and mechanical stretch of different tissues research. It is evident that the data, available on this topic is scarce, and this Volume is especially valuable, since its 10 Chapters provide a complete description of the modern vision of the field, which is rapidly developing. Volume begins with an overview of cytokines, addressed to those readers, who would like to get acquainted with this topic. In following Chapters authors summarize the recent findings about the cellular and molecular effect of mechanical stretch, which induce cytokine production in cardiomyocytes, fibroblasts, vascular smooth muscle cells, endothelial cells, lung and chondrocytes. Following description of mechanosensitive proinflammatory gene expression is of special interest. Chapters describing the role of major v vi Foreword

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Mechanosensitivity and Mechanotransduction

Cited by 8 publications

References 21 publications

Revealing hidden information in osteoblast’s mechanotransduction through analysis of time patterns of critical events

Revealing hidden information in osteoblast’s mechanotransduction through analysis of time patterns of critical events

Nanoporous Gold Biointerfaces: Modifying Nanostructure to Control Neural Cell Coverage and Enhance Electrophysiological Recording Performance

Mechanical Stretch and Cytokines

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