Media Composition, Microbial, Laboratory Scale

Combined approach of NMR and chemometrics for screening peptones used in the cell culture medium for the production of a recombinant therapeutic protein

“…In the biopharmaceutical industry, concerns over transmissible spongiform encephalopathies (TSEs) and other adventitious agents from using animal-derived components have prompted the elimination of animal-derived components and implementation of new supplements (Heidemann et al, 2000;Keenan et al, 2006;Xie et al, 1997;Zhang et al, 1994). Soy peptones as substitutes for serum have been successfully used in cell culture medium formulation for over 30 years (Cote, 1999;Taylor et al, 1972).…”