Media for Isolation of Aeromonas Spp. from Faeces

Robinson, J. S.; Burke, Valerie; Worthy, P.J.; Beaman, J; Wagener, Linda

doi:10.1099/00222615-18-3-405

Cited by 24 publications

(8 citation statements)

References 15 publications

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“…Other researchers previously recommended the routine use of BA in the detection more than two enteric of Aeromonas spp. in feces (6,9,10,15). Our data support this recommendation.…”

Section: Resultssupporting

confidence: 83%

Growth of Aeromonas species on enteric agars

Desmond¹,

Janda²

1986

J Clin Microbiol

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The efficacy of eight routine enteric agars for supporting the growth of 32 strains of Aeromonas spp. (17 A. hydrophila strains, 8 A. sobria strains, and 7 A. caviae strains) was investigated. The plating efficiency of Aeromonas spp. on these media varied greatly (range, 0 to 100%), as did their colony size when compared with that on noninhibitory medium (5% sheep blood agar). Plating efficiency on seven of these eight media appeared to be strain-and not species dependent. Overall, eosin-methylene blue and Hektoen enteric agars showed low plating efficiencies for A. hydrophila, whereas both A. sobria and A. caviae were severely inhibited on brilliant green agar. When all these species are considered collectively, deoxycholate, MacConkey, and xylose lysine deoxycholate appeared to be the most satisfactory routine agars for Aeromonas spp. recovery when used in

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“…Other researchers previously recommended the routine use of BA in the detection more than two enteric of Aeromonas spp. in feces (6,9,10,15). Our data support this recommendation.…”

Section: Resultssupporting

confidence: 83%

Growth of Aeromonas species on enteric agars

Desmond¹,

Janda²

1986

J Clin Microbiol

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“…By contrast the application of non-selective media has enabled the isolation of Aeromonas strains which are sensitive to selective agents (Holmberg and Farmer, 1984;Desmond and Janda, 1986;Holmberg et al, 1986). Other researchers (Gracey et al, 1982;Janda et ah, 1984;Robinson et al, 1984) recommended the routine use of blood agar in the detection of Aeromonas spp. No selective media were used in the present study because of these facts, and the desire to isolate as broad a spectrum of bacteria as possible.…”

Section: Resultsmentioning

confidence: 99%

Iccurrence ofaeromonas hydrophilain wild birds

Glünder

Siegmann

1989

Avian Pathology

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SUMMARYAeromonas hydrophila was isolated from 135 of a total of 1226 wild birds. It was the only bacterial species in eight birds, while other bacteria, mainly enterobacteriaceae, staphylococci and/or streptococci were identified in all other birds. The rate of isolation from aquatic birds (18.5%) was higher (P<0.001) than from birds of terrestrial habitats (3.4%). Infection may also depend on dietary habits: 7.0% of the granivorous and herbivorous species, 8.4% of the omnivores and 12.0% of carnivorous and insectivorous birds were infected. A. hydrophila was isolated more frequently during the summer (12.9%) than the winter (8.9%).

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“…The oxidase test is recommended for screening Enterobacteriaceae from bacteria in other families. Although Aeromonas hydrophila can ferment lactose, the byproducts of lactose metabolism on EMB plates strongly inhibit the growth of Aeromonas, and its colony-forming ability on EMB plates is insignificant (Millership and Chattapadhya 1984;Robinson et al 1984;Kay et al 1985). Therefore, colonies from EMB plates could be safely taken through the biochemical tests for E. coli.…”

Section: Methodsmentioning

confidence: 99%

Microbial Pollution in Water and Its Effect on Fish

Pal

Gupta

1992

Journal of Aquatic Animal Health

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A systematic study of microbial contamination offish grown in ponds in and around Calcutta was initiated as pan of the wetland management project in the city. Ponds were either sewage-fed or conventional (i.e., fed by rain and groundwater). Fish from these ponds are used for human consumption, and therefore a quantitative analysis of their microbial content is necessary. When concentrations of Escherichia coli, Salmonelleae (unidentified species), and Staphylococcus aureus in water and different organs of fish from sewage-fed and conventional ponds were compared, water and fish organs from conventional ponds contained about two orders of magnitude more bacterial cells. We tested for Salmonelleae and 5. aureus, in addition to E. coli, because of persistent reports from Calcutta hospitals that most enteric and other infectious diseases in this region are caused by these bacteria. Significant linear correlations were found between concentrations of these bacteria in pond water and their recovery from several tissues of the fish.

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Media for Isolation of Aeromonas Spp. from Faeces

Cited by 24 publications

References 15 publications

Growth of Aeromonas species on enteric agars

Growth of Aeromonas species on enteric agars

Iccurrence ofaeromonas hydrophilain wild birds

Microbial Pollution in Water and Its Effect on Fish

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