Mediated amperometric enzyme electrode incorporating peroxidase for the determination of hydrogen peroxide in organic solvents

Schubert, Florian; Saini, Selwayan; Turner, Anthony P. F.

doi:10.1016/s0003-2670(00)80212-3

Cited by 103 publications

(21 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HRP-electrodes have been used successfully to measure peroxides in organic-phase environments, most reported systems relying on the use of mediators to transfer electrons between the reducing electrode and prosthetic haem group within the HRP molecule. Schubert et al [89,90] were able to quantify hydrogen peroxide in chloroform and dioxane by co-immobilisation of HRP and water-soluble hexacyanoferrate at a carbon electrode. Adeyoju et al [91] have measured butanone peroxide at an HRP-platinum electrode in 98% v/v acetonitrile in phosphate buffer using o-phenyldiamine as the mediator.…”

Section: Enzymes and Enzyme Electrochemistry In The Organic-phasementioning

confidence: 99%

Organic phase immunosensors

Setford¹,

Kröger²,

Turner³

1999

Analusis

View full text Add to dashboard Cite

Many analytical methods involving non-polar analytes require solvent extraction prior to measurement. The analysis procedure is greatly simplified if the method is able to function effectively in the more non-polar solvent extract. This consideration, coupled with the increasing need for simple, specific and rapid diagnostic and screening tools, has focused interest in the development of organic-phase immunosensors.

show abstract

Section: Enzymes and Enzyme Electrochemistry In The Organic-phasementioning

confidence: 99%

Organic phase immunosensors

Setford¹,

Kröger²,

Turner³

1999

Analusis

View full text Add to dashboard Cite

show abstract

“…MO) and used as received. Titanium isopropoxide (Ti(iPrO) 4 ) was obtained from Aldrich. Phenol, catechol, pcresol, and chloroform were from Beijing Chemical Factory (Beijing, China).…”

Section: Reagentsmentioning

confidence: 99%

“…When water-miscible solvents are used, the enzyme loses its activity in most instances due to the distortion of the essential water layer by the solvent. The works reported by Dong [7] and Turner [4] discovered that less water is desorbed from enzyme molecule in solvent with log P > 2 (P is the partition coefficient of a given solvent between noctanol and water in a two-phase system). Therefore, the enzyme can keep the essential water and its bioactivity in hydrophobic solvents such as chloroform and chlorobenzene.…”

Section: Optimization Of the Experimental Parametersmentioning

confidence: 99%

See 1 more Smart Citation

Pure Organic Phase Phenol Biosensor Based on Tyrosinase Entrapped in a Vapor Deposited Titania Sol‐Gel Membrane

Yu¹,

Ju²

2004

Electroanalysis

View full text Add to dashboard Cite

A novel amperometric biosensor was constructed for the determination of phenols in pure organic phase. This biosensor was fabricated by immobilizing tyrosinase in a titania sol-gel membrane which was obtained with a vapor deposition method. This method was facile and avoided the calcination step needed in conventional titania sol-gel process. The titania sol-gel membrane could effectively retain the essential water layer around the enzyme molecule needed for maintaining its activity in organic phase. The experimental parameters such as solvent and operating potential were optimized. At À 100 mV this biosensor showed a good amperometric response to phenols in pure chloroform without any mediator and rehydration of the enzyme. For catechol determination the sensor exhibited a fast response of less than 5 seconds. The sensitivity of different phenols was as follows: catechol > phenol > p-cresol. Additionally, the apparent Michaelis-Menten constants of the encapsulated tyrosinase to catechol, phenol and pcresol were found to be 0.15 AE 0.003, 0.17 AE 0.008 and 0.21 AE 0.004 mM, respectively. The biosensor had also good reproducibility and stability. This work provided a promising platform for the construction of pure organic phase biosensors and the determination of substrates with poor water solubility.

show abstract

“…The HRP modified electrodes have been constructed by using either direct [5][6][7][8][9] or mediate-assisted [10][11][12] electron transfer from FADH 2 centers of HRP. Generally, direct electron transfer between common electrode materials and immobilized HRP is a slow process while electron transfer via a mediator presented in solution, called as the secondgeneration biosensors [13,14], is more effective in the bioelectrocatalytic reduction of peroxides at HRP-based sensors with detection limit as low as 10 ¹7 -10 ¹8 mol L ¹1 .…”

Section: Introductionmentioning

confidence: 99%

A Reagentless Hydrogen Peroxide Biosensor Based on the Coimmobilization of Thionine and Horseradish Peroxidase by Their Cross-Linking with Glutaraldehyde on Glassy Carbon Electrode

Zhou

Chen

1998

Electroanalysis

View full text Add to dashboard Cite

A novel reagentless H 2 O 2 sensor, based on the coimmobilization of horseradish peroxidase (HRP) and the redox dye thionine by their cross-linking with glutaraldehyde on the surface of a glassy carbon electrode, was fabricated. This resulting modified electrode exhibited good electrochemical activity in aqueous solution, and the electron transfer rate constant between the derivative thionine and glassy carbon electrode was about 0.097 s ¹1 (calculated from Laviron method). The apparent surface coverage of thionine was about 8.54 × 10 ¹12 mol cm ¹2 . It was found that thionine could mediate the electron transfer of HRP with the electrode substrate. Performance and characteristics of the biosensor were evaluated with respect to response time, detection limit, applied potential, pH, and temperature. The results inidcate that this biosensor is of high sensitivity to hydrogen peroxide with a low detection limit down to 1.0 × 10 ¹7 mol L ¹1 . Moreover, dopamine, ascorbic acid, and uric acid did not interfere with the determination of H 2 O 2 . Meanwhile, this enzyme electrode also showed good stability and reproducibility for long-term use.

show abstract

Mediated amperometric enzyme electrode incorporating peroxidase for the determination of hydrogen peroxide in organic solvents

Cited by 103 publications

References 14 publications

Organic phase immunosensors

Organic phase immunosensors

Pure Organic Phase Phenol Biosensor Based on Tyrosinase Entrapped in a Vapor Deposited Titania Sol‐Gel Membrane

A Reagentless Hydrogen Peroxide Biosensor Based on the Coimmobilization of Thionine and Horseradish Peroxidase by Their Cross-Linking with Glutaraldehyde on Glassy Carbon Electrode

Contact Info

Product

Resources

About