Medicago TERPENE SYNTHASE 10 Is Involved in Defense Against an Oomycete Root Pathogen

Abstract

H.Y. and D.D. contributed to project conception and development, designed and performed experiments, and analyzed and interpreted the data; B.A analyzed transcriptomics data; A.

Functional characterization of a defense‐responsive bulnesol/elemol synthase from potato

“…Several studies have indicated the induction of TPS genes in response to various pathosystems implicating their role in defense (Attaran et al 2008, Li et al 2015, Yadav et al 2019). To examine the terpenes released from potato plants, leaves of 4‐week old plant were infiltrated with P. syringae .…”

“…As sedentary organisms, plants have evolved different mechanisms to tackle the attack by pathogens. It has been reported that microbial infection induces expression of genes encoding TPSs responsible for volatile terpenoids in different plant species including potato (Yoshioka et al 1999, Attaran et al 2008, Li et al 2015, Lee et al 2017, Yadav et al 2019). Hence, to identify and characterize TPSs induced in response to microbial infection in potato leaf, analysis of terpene volatiles and expression of candidate genes identified from potato genome was carried out.…”

“…Recently, a multiproduct sesquiterpene synthase (PhTPS1) from petunia was shown to defend the developing stigma from pathogens (Boachon et al 2019). In Medicago truncatula , Yadav et al (2019) demonstrated defense role of a multiproduct terpene synthase MtTPS10 during Aphanomyces euteiches infection. All of these above studies clearly showed the modulation of plant defense against bacterial and fungal pathogens through TPSs and their enzymatic terpenoid products.…”

The Sesquiterpene Synthase PtTPS5 Produces (1S,5S,7R,10R)-Guaia-4(15)-en-11-ol and (1S,7R,10R)-Guaia-4-en-11-ol in Oomycete-Infected Poplar Roots

“…Infection of strawberry ( Fragaria vesca ) roots with P. cactorum has been shown to induce massive changes in the transcriptome, including the upregulation of the complete mevalonate pathway, two FPP synthase genes, and four putative sesquiterpene synthase genes with similarity to germacene D synthase [ 25 ]. Moreover, Yadav and colleagues reported that the infection of Medicago truncatula roots with the oomycete Aphanomycus euteiches led also to the expression of a sesquiterpene synthase gene [ 26 ]. The encoded enzyme MtTPS10 was shown to produce a blend of sesquiterpenes with the alcohol himachalol as the major component.…”

“…Down regulation of MtTPS10 resulted in increased susceptibility to the oomycete and a mixture of isolated MtTPS10 products inhibited mycelial growth and A. euteiches zoospore germination. However, since himachalol could not be detected in A. euteiches -infected roots, MtTPS10 alcohols are likely converted to other terpenoids as speculated by the authors [ 26 ]. Indeed, conversion of sesquiterpenes into polar compounds such as aldehydes and acids upon pathogen infection has been described in a number of plants.…”