Medical methods and devices for outpatient monitoring of human health

Viktorov, Vladimir; Матвеев, Э. В.; Gundarov, V. P.; Varin, Audrey

doi:10.1007/bf02388375

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To underscore the degree of miniaturization allowed by the soft lithography technique, figure 4(c) demonstrates the size difference between a commercially available 'macro' SEC column (SRT SEC-300, SEPAX Technologies) and the fabricated LC mini-column. The mini-column's width, length and height are only 5 × 5 × 0.02 mm 3 , respectively, a reduction factor of ∼11 000 from the macro-scale column's inner diameter and length: 4.6 × 300 mm 2 .…”

Section: Bead-packing Techniques In the Lc Mini-columnmentioning

confidence: 98%

“…The ability to diagnose and monitor chronic health conditions like heart disease, diabetes, high blood pressure, cholesterol and cancer [1,2] is a key concern in medicine. Patients with these conditions are initially diagnosed with bloodwork and, once diagnosed, require regular blood monitoring [3][4][5] of the many health index markers found in blood. For reliability, convenience, and efficiency, implantable monitoring devices would be an obvious solution.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Miniaturized protein separation using a liquid chromatography column on a flexible substrate

Yang¹,

Chae²

2008

J. Micromech. Microeng.

View full text Add to dashboard Cite

We report a prototype protein separator that successfully miniaturizes existing technology for potential use in biocompatible health monitoring implants. The prototype is a liquid chromatography (LC) column (LC mini-column) fabricated on an inexpensive, flexible, biocompatible polydimethylsiloxane (PDMS) enclosure. The LC mini-column separates a mixture of proteins using size exclusion chromatography (SEC) with polydivinylbenzene beads (5-20 μm in diameter with 10 nm pore size). The LC mini-column is smaller than any commercially available LC column by a factor of ∼11 000 and successfully separates denatured and native protein mixtures at ∼71 psi of the applied fluidic pressure. Separated proteins are analyzed using NuPAGE-gel electrophoresis, high-performance liquid chromatography (HPLC) and an automated electrophoresis system. Quantitative HPLC results demonstrate successful separation based on intensity change: within 12 min, the intensity between large and small protein peaks changed by a factor of ∼20. In further evaluation using the automated electrophoresis system, the plate height of the LC mini-column is between 36 μm and 100 μm. The prototype LC mini-column shows the potential for real-time health monitoring in applications that require inexpensive, flexible implant technology that can function effectively under non-laboratory conditions.

show abstract

Section: Bead-packing Techniques In the Lc Mini-columnmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Miniaturized protein separation using a liquid chromatography column on a flexible substrate

Yang¹,

Chae²

2008

J. Micromech. Microeng.

View full text Add to dashboard Cite

show abstract

“…An automated system for preliminary examination developed at VNIIMP VITA, Ltd., Russian Academy of Medical Sciences, was used [3,5]. The system is designed to provide health monitoring in healthy subjects and patients with chronic diseases without exacerbation.…”

Section: Apparatusesmentioning

confidence: 99%