Medical ozone therapy reduces shock wave therapy-induced renal injury

Abstract

Objectives: Extracorporeal shock wave (ESW) lithotripsy is the preferred treatment modality for uncomplicated kidney stones. More recently free oxygen radical production following ESW application has been considered to be crucial in shock wave-induced renal damage. It has been shown that ozone therapy (OT) has ameliorative and preventive effects against various pathological conditions due to increased nitro-oxidative stress. In current study, we aimed to evaluate the efficacy of OT against ESW-induced renal injury. Methods: Twenty-four male Sprague-Dawley rats were divided into three groups: sham-operated, ESW, and ESW þ OT groups. All groups except shamoperated group were subjected to ESW procedure. ESW þ OT group received 1 mg/kg/day of oxygen/ozone mixture intraperitoneally at 2 h before ESW, and OT was continued once a day for consecutive three days. The animals were killed at the 4th day, and kidney tissue and blood samples were harvested for biochemical and histopathologic analysis. Results: Serum ALT and AST levels, serum neopterin, tissue nitrite/nitrate levels, and tissue oxidative stress parameters were increased in the ESW group and almost came close to control values in the treatment group (p < 0.05, ESW vs. ESW þ OT). Histopathological injury scores were significantly lower in treatment group than the ESW group (p < 0.05, ESW vs. ESW þ OT). Immunohistochemical iNOS staining scores in ESW group were higher than those of sham-operated group (p < 0.05, ESW vs. sham-operated), iNOS staining scores in OT group were significantly lower than the ESW group (p < 0.05, ESW þ OT vs. ESW). Conclusion: OT ameliorates nitro-oxidative stress and reduces the severity of pathological changes in the experimental ESW-induced renal injury of rat model. ARTICLE HISTORY

A pilot study evaluating changes to haematological and biochemical tests after Flexible Ureterorenoscopy for the treatment of kidney stones

“…Overall it can be appreciated that further detailed investigations into the inflammatory process and kidney function post FURS is needed, as recent evidence links prolonged inflammation to scaring, which in combination with oxidative stress, contributes to the pathogenesis of diabetes mellitus, hypertension and diabetic nephropathy [27]. Work by Uğuz S et al, (2016) explored the effect on ozone therapy in reducing lithotripsy induced renal injury on rats during SWL and found that administering ozone resulted in significant changes to biochemical results, notably a reduction is AST and ALP, as well as ameliorating nitro-oxide stress [28]. Therefore, future monitoring for sustained inflammation combined with the potential for therapeutic intervention such as ozone therapy may lead to better patient outcomes.…”

The Protective Role of Ozone Therapy in Kidney Disease: A Review

“…Nevertheless, adverse effects such as hematuria might be present after the procedure [ 98 ]. Experimentally, O 3 treatment has been proven as effective against the morphological and oxidative damage caused by shock wave therapy [ 99 ]. The novel therapy, due to its antimicrobial capacity, has also ameliorated oxidative damage caused by microorganisms in kidney infection (pyelonephritis) [ 88 ] and septic shock in kidneys [ 100 ], as well as in other organs [ 101 ].…”