Medicinal compounds produced in plant cell factories.

Hakkinen, ST; Ritala, Anneli; Arora, Rajesh

doi:10.1079/9781845936785.0013

Cited by 8 publications

(5 citation statements)

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“…In comparison to cell suspensions, HRCs present several advantages such as genotypic and phenotypic stability and possible extracellular secretion of expressed proteins (also referred to as rhizosecretion) offering a convenient method for target proteins purification in a well-defined protein-deficient medium (Wang and Wu, 2013). HRCs are capable for the production of complex compounds and high scalability (Häkkinen and Ritala, 2010;Stoger et al, 2014). In this context, the production of recombinant proteins has been considered a promising application of HRCs.…”

Section: Introductionmentioning

confidence: 99%

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Gutierrez-Valdes

Häkkinen

Lemasson³

et al. 2020

Front. Plant Sci.

187

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Hairy roots derived from the infection of a plant by Rhizobium rhizogenes (previously referred to as Agrobacterium rhizogenes) bacteria, can be obtained from a wide variety of plants and allow the production of highly diverse molecules. Hairy roots are able to produce and secrete complex active glycoproteins from a large spectrum of organisms. They are also adequate to express plant natural biosynthesis pathways required to produce specialized metabolites and can benefit from the new genetic tools available to facilitate an optimized production of tailor-made molecules. This adaptability has positioned hairy root platforms as major biotechnological tools. Researchers and industries have contributed to their advancement, which represents new alternatives from classical systems to produce complex molecules. Now these expression systems are ready to be used by different industries like pharmaceutical, cosmetics, and food sectors due to the development of fully controlled large-scale bioreactors. This review aims to describe the evolution of hairy root generation and culture methods and to highlight the possibilities offered by hairy roots in terms of feasibility and perspectives.

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Section: Introductionmentioning

confidence: 99%

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Gutierrez-Valdes

Häkkinen

Lemasson³

et al. 2020

Front. Plant Sci.

187

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“…Plant‐based production systems can be used to manufacture valuable pharmaceutical and industrial proteins, an approach known as molecular farming (Häkkinen and Ritala, ; Ma et al, ). The advantages of plants include the cost‐effective propagation of biomass, the ability to carry out complex post‐translational modifications similar to mammalian cells, and the absence of endotoxins and human pathogens (Twyman et al, ).…”

Section: Introductionmentioning

confidence: 99%

Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody

Häkkinen

Raven

Henquet³

et al. 2013

Biotech & Bioengineering

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Recombinant pharmaceutical proteins expressed in hairy root cultures can be secreted into the medium to improve product homogeneity and to facilitate purification, although this may result in significant degradation if the protein is inherently unstable or particularly susceptible to proteases. To address these challenges, we used a design of experiments approach to develop an optimized induction protocol for the cultivation of tobacco hairy roots secreting the full-size monoclonal antibody M12. The antibody yield was enhanced 30-fold by the addition of 14 g/L KNO3 , 19 mg/L 1-naphthaleneacetic acid and 1.5 g/L of the stabilizing agent polyvinylpyrrolidone. Analysis of hairy root cross sections revealed that the optimized medium induced lateral root formation and morphological changes in the inner cortex and pericycle cells, indicating that the improved productivity was at least partially based on the enhanced efficiency of antibody secretion. We found that 57% of the antibody was secreted, yielding 5.9 mg of product per liter of induction medium. Both the secreted and intracellular forms of the antibody could be isolated by protein A affinity chromatography and their functionality was confirmed using vitronectin-binding assays. Glycan analysis revealed three major plant complex-type glycans on both forms of the antibody, although the secreted form was more homogeneous due to the predominance of a specific glycoform. Tobacco hairy root cultures therefore offer a practical solution for the production of homogeneous pharmaceutical antibodies in containment.

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“…Future work will therefore focus on the establishment of further hairy root clones starting from high yielding Edelweiss genotypes, and using additional A. rhizogenes strains. In any case, as a continuous, sustainable and renewable production system independent of climatic or environmental effects [45] the biotechnological production of leoligin and 5-methoxy-leoligin would be of advantage over the extraction from field grown plants.…”

Section: Discussionmentioning

confidence: 99%

Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter)

et al. 2014

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A hairy root line of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter) was obtained upon transformation with Agrobacterium rhizogenes strain ATCC15834. Elicitation of this line with silver nitrate, sucrose, methyl jasmonate and yeast extract at various concentrations in most cases resulted in a stimulation of lignan biosynthesis. Through elicitation with 6% sucrose the roots accumulated the pharmacologically active lignans leoligin and 5-methoxy-leoligin at levels of 0.0678% and 0.0372%, respectively, without significant growth inhibition. These lignan levels were comparable to those found in intact roots of cultivated Edelweiss. The biotechnological production of leoligin could be an attractive option for the continuous, field culture-independent production of the valuable secondary metabolites leoligin and 5-methoxy-leoligin.

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Medicinal compounds produced in plant cell factories.

Cited by 8 publications

References 0 publications

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody

Lignan formation in hairy root cultures of Edelweiss (Leontopodium nivale ssp. alpinum (Cass.) Greuter)

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