Medicinal Foodstuffs. XXXIV. Structures of New Prenylchalcones and Prenylflavanones with TNF-.ALPHA. and Aminopeptidase N Inhibitory Activities from Boesenbergia rotunda

Bioactive Constituents from Chinese Natural Medicines. XXXII. Aminopeptidase N and Aldose Reductase Inhibitors from Sinocrassula indica: Structures of Sinocrassosides B4, B5, C1, and D1-D3

“…Effects on Aminopeptidase N Inhibitory Activity Aminopeptidase N activity was assayed by the method described in a previous report. 24) Briefly, 0.2 mM of the enzyme substrate, L-alanine-4-methylcoumaryl-7-amide (Ala-MCA, Peptide Institute) in 50 mM Tris-HCl buffer containing 200 mM NaCl (pH 8.0, 100 ml/well) and sample solution (20 ml) in 96-well black microplates. The reaction mixture was initiated by adding an enzyme solution 100 ml/well (rat, 1 mU, Calbiochem) and incubated at 37°C for 1 h to convert Ala-MCA to a fluorescent product, 7-amino-4-methylcoumarin (AMC).…”

“…Previously, we have reported the isolation and structure elucidation of several flavonoids with APN inhibitory activity from the rhizomes of Boesenbergia rotunda. 24) As shown in Table 3, most of them except for megastigmane constituents (33, 34) were found to inhibit APN activity.…”

An Overview on Cardamonin

“…3 Cardamonin's name comes from the fact that it can be found in cardamom spice (Fig. 4), but since the first article where it was described, 22 it has been found in many other (some of them edible) plant species, such as Alpinia blepharocalyx, 23,24 Alpinia gagnepainii, 25 Alpinia conchigera, 26,27 Alpinia hainanensis, [28][29][30][31][32][33][34][35][36][37][38][39][40] Alpinia malaccensis, 41 Alpinia mutica, 42,43 Alpinia pricei, 44 Alpinia rafflesiana, [45][46][47][48] Alpinia speciosa, 22,49,50 Amomum subulatum, 51 Artemisia absinthium, 52 Boesenbergia pandurata, [53][54][55][56][57][58][59][60] Boesenbergia rotunda, 61,62 Carya cathayensis, 63 Cedrelopsis grevei, 64 Combretum apiculatum, 65 Comptonia pere...…”

“…They obtained a result of 6% inhibition of the N-hydroxylation of 3-amino-1-methyl-5H-pyrido [4,3-b]indole, with methanol being the control. Morikawa et al 62 obtained with a 30 lM, a 37% inhibition on the aminopeptidase N activity (a metalloprotease that plays an important role in tumor cell invasion, extracellular and tumor metastasis), curcumin being the reference compound.…”

Structures of New Phenylbutanoids and Nitric Oxide Production Inhibitors from the Rhizomes of Zingiber cassumunar

“…[1][2][3][4][5] For example, several phenylbutanoids isolated from Z. cassumunar were reported to possess P-glycoprotein inhibitory, 1) cyclooxygenase-2 inhibitory, 4) and anti-inflammatory 5) effects. During the course of our characterization studies on Zingiberaceae natural medicines, [6][7][8][9][10][11][12][13][14][15][16] the MeOH extract from the rhizomes of Z. cassumunar was found to show inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse peritoneal macrophages. From the MeOH extract, six new phenylbutanoids named phlains I-VI were isolated together with 16 known constituents.…”