Medicinal parasitic plants on diverse hosts with their usages and barcodes

Abstract

Medicinal properties of parasitic plants were investigated by means of ethnobotanical study in some areas of northeastern Thailand. Important traditional usages are: Scurrula atropurpurea nourishes blood, Dendrophthoe pentandra decreases high blood pressure, and Helixanthera parasitica treats liver disease. Their systematics were also determined. The research is based on findings obtained from 100 parasite-host pairs. Of these, eight parasitic species were recorded; they are members of two families, viz. family Loranthaceae, namely D. lanosa, D. pentandra, H. parasitica, Macrosolen brandisianus, M. cochinchinensis and S. atropurpurea, and family Viscaceae, namely Viscum articulatum and V. ovalifolium. In addition, each parasitic species is found on diverse hosts, indicating non-host-parasitic specificity. Species-specific tagging of all species studied was carried out using the rbcL and psbA-trnH chloroplast regions. These tag sequences are submitted to GenBank databases under accession numbers JN687563-JN687578. Genetic distances calculated from nucleotide variations in a couple of species of each genus, Dendrophthoe, Macrosolen, and Viscum, were 0.032, 0.067 and 0.036 in the rbcL region, and 0.269, 0.073 and 0.264 in the psbA-trnH spacer region, respectively. These variations will be used for further identification of incomplete plant parts or other forms such as capsule, powder, dried or chopped pieces.

Dna Barcode Characterization of Mistletoe Infestation in Teak Clonal Seed Orchard (Cso) in Padangan, East Java Province, Indonesia

“…On the other hand, interspecific variations of rbcL region were 0.064 and 0.042 for Loranthaceae and Santalaceae, respectively. Value of interspecific distances of rbcL region for Loranthaceae and Santalaceae reported in this study were similar to the results reported by Kwanda et al (2013), which was 0.032 -0.067.…”

“…In this study, an average sequence length of rbcL region was 738 -740 bp. This value was higher than the length reported by Kwanda et al (2013) who used the same rbcL region, but different primer base pairs (5'-ATGTCACCACAAACAGAGACTAAAGC-3' and 5'-GTAAAATCAAGTCCACCRCG-3'), showing 550 bp for Loranthaceae family (D. pentandra, D. lanosa, Scurrula atropurpurea, M. cochinchinensis, M. bradisianus, Helixanthera parasitica) and Santalaceae family (V. articulatum and V. ovalifolium).…”

“…The first attempt to assess parasite attack patterns and distribution in sampled compartments of teak stands and a Clonal Seed Orchard (CSO) was conducted by Muttaqin et al the treatment of certain diseases should have a specific barcode DNA marker to guarantee the correctness of medical treatment (Kwanda . et al 2013).…”

DNA profiles to identify Dillenia species (Dilleniaceae) in Thailand

“…philippinensis (2) D. pulchella (1) (Kwanda et al 2013). Additionally, the matK gene marker has been identified in some medicinal Piper species .…”

“…DNA barcoding is the most reliable and applicable method for overcoming this problem (Hebert et al 2003, CBOL Plant Working Group 2009, Hollingsworth et al 2011. Several research groups suggested appropriate DNA barcoding regions in some plant groups, such as the matK gene (Siripiyasing et al 2012, the rbcL gene , Kwanda et al 2013, and the psbA-trnH spacer region . Standard barcodes used for most plant groups are matK gene, rbcL gene, psbA-trnH spacer, and ITS region identified by the CBOL Plant Working Group (2009), Chaveerach et al (2011), Hollingsworth et al (2011 and Monkheang et al (2011).…”